Insects as biological models to assay spider and scorpion venom toxicity

Detalhes bibliográficos
Autor(a) principal: Manzoli-palma, M. F. [UNESP]
Data de Publicação: 2003
Outros Autores: Gobbi, N. [UNESP], Palma, M. S. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992003000200004
http://hdl.handle.net/11449/212375
Resumo: This study was undertaken to develop an experimental protocol using insects as biological models to assay venom toxicity of the following spiders Loxosceles gaucho, Phoneutria nigriventer, Nephilengys cruentata and Tityus serrulatus scorpion. Three different insect species were bioassayed: Apis mellifera (Hymenoptera), Grillus assimilis (Orthoptera), and Diatraea saccharalis (Lepidoptera). Venoms were injected into the hemocele of insects with a microsyringe at concentrations that caused dose/weight-dependent effects; doses causing either paralysis (ED50) or death (LD50) were recorded for each venom and insect test-species. T. serrulatus and L. gaucho venoms were lethal to all tested species, while P. nigriventer venom caused paralysis and death, and N. cruentata venom caused only paralysis at the doses assayed. A comparison between the insect test species described above revealed that A. mellifera was highly sensitive to all venoms tested; even a tiny amount of N. cruentata non-lethal venom caused a change in the walking pattern leading to transient paralysis. D. saccharalis larvae were very resistant to all four venoms.
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spelling Insects as biological models to assay spider and scorpion venom toxicitybioassayvenominsect modeltoxicityApis melliferaThis study was undertaken to develop an experimental protocol using insects as biological models to assay venom toxicity of the following spiders Loxosceles gaucho, Phoneutria nigriventer, Nephilengys cruentata and Tityus serrulatus scorpion. Three different insect species were bioassayed: Apis mellifera (Hymenoptera), Grillus assimilis (Orthoptera), and Diatraea saccharalis (Lepidoptera). Venoms were injected into the hemocele of insects with a microsyringe at concentrations that caused dose/weight-dependent effects; doses causing either paralysis (ED50) or death (LD50) were recorded for each venom and insect test-species. T. serrulatus and L. gaucho venoms were lethal to all tested species, while P. nigriventer venom caused paralysis and death, and N. cruentata venom caused only paralysis at the doses assayed. A comparison between the insect test species described above revealed that A. mellifera was highly sensitive to all venoms tested; even a tiny amount of N. cruentata non-lethal venom caused a change in the walking pattern leading to transient paralysis. D. saccharalis larvae were very resistant to all four venoms.Universidade Estadual Paulista, Institute of Biosciences of Rio ClaroUniversidade Estadual Paulista, Center of Environmental StudiesUniversidade Estadual Paulista, Center for the Study of Venoms and Venomous AnimalsUniversidade Estadual Paulista, Institute of Biosciences of Rio ClaroUniversidade Estadual Paulista, Center of Environmental StudiesUniversidade Estadual Paulista, Center for the Study of Venoms and Venomous AnimalsCentro de Estudos de Venenos e Animais PeçonhentosUniversidade Estadual Paulista (Unesp)Manzoli-palma, M. F. [UNESP]Gobbi, N. [UNESP]Palma, M. S. [UNESP]2021-07-14T10:39:01Z2021-07-14T10:39:01Z2003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article174-185http://dx.doi.org/10.1590/S1678-91992003000200004Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 9, n. 2, p. 174-185, 2003.1678-9199http://hdl.handle.net/11449/21237510.1590/S1678-91992003000200004S1678-91992003000200004S1678-91992003000200004.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-04-10T19:22:34Zoai:repositorio.unesp.br:11449/212375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:07:18.697266Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Insects as biological models to assay spider and scorpion venom toxicity
title Insects as biological models to assay spider and scorpion venom toxicity
spellingShingle Insects as biological models to assay spider and scorpion venom toxicity
Manzoli-palma, M. F. [UNESP]
bioassay
venom
insect model
toxicity
Apis mellifera
title_short Insects as biological models to assay spider and scorpion venom toxicity
title_full Insects as biological models to assay spider and scorpion venom toxicity
title_fullStr Insects as biological models to assay spider and scorpion venom toxicity
title_full_unstemmed Insects as biological models to assay spider and scorpion venom toxicity
title_sort Insects as biological models to assay spider and scorpion venom toxicity
author Manzoli-palma, M. F. [UNESP]
author_facet Manzoli-palma, M. F. [UNESP]
Gobbi, N. [UNESP]
Palma, M. S. [UNESP]
author_role author
author2 Gobbi, N. [UNESP]
Palma, M. S. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Manzoli-palma, M. F. [UNESP]
Gobbi, N. [UNESP]
Palma, M. S. [UNESP]
dc.subject.por.fl_str_mv bioassay
venom
insect model
toxicity
Apis mellifera
topic bioassay
venom
insect model
toxicity
Apis mellifera
description This study was undertaken to develop an experimental protocol using insects as biological models to assay venom toxicity of the following spiders Loxosceles gaucho, Phoneutria nigriventer, Nephilengys cruentata and Tityus serrulatus scorpion. Three different insect species were bioassayed: Apis mellifera (Hymenoptera), Grillus assimilis (Orthoptera), and Diatraea saccharalis (Lepidoptera). Venoms were injected into the hemocele of insects with a microsyringe at concentrations that caused dose/weight-dependent effects; doses causing either paralysis (ED50) or death (LD50) were recorded for each venom and insect test-species. T. serrulatus and L. gaucho venoms were lethal to all tested species, while P. nigriventer venom caused paralysis and death, and N. cruentata venom caused only paralysis at the doses assayed. A comparison between the insect test species described above revealed that A. mellifera was highly sensitive to all venoms tested; even a tiny amount of N. cruentata non-lethal venom caused a change in the walking pattern leading to transient paralysis. D. saccharalis larvae were very resistant to all four venoms.
publishDate 2003
dc.date.none.fl_str_mv 2003
2021-07-14T10:39:01Z
2021-07-14T10:39:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992003000200004
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 9, n. 2, p. 174-185, 2003.
1678-9199
http://hdl.handle.net/11449/212375
10.1590/S1678-91992003000200004
S1678-91992003000200004
S1678-91992003000200004.pdf
url http://dx.doi.org/10.1590/S1678-91992003000200004
http://hdl.handle.net/11449/212375
identifier_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 9, n. 2, p. 174-185, 2003.
1678-9199
10.1590/S1678-91992003000200004
S1678-91992003000200004
S1678-91992003000200004.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 174-185
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129393995808768

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