Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposure
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00709-019-01347-5 http://hdl.handle.net/11449/188625 |
Resumo: | The effects of biopesticides on insects can be demonstrated by morphological and ultrastructural tools in ecotoxicological analysis. Azadirachtin-based products are widely used as biopesticides, affecting numerous insect populations. Through morphological biomarkers, this study aimed to characterize the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri after chronic exposure to azadirachtin. Larvae of S. eridania and C. claveri were fed with fresh purple lettuce leaves (Lactuca sativa) and egg clusters of Diatraea saccharalis treated with azadirachtin solution of 6 mg active ingredient (a.i.)/L and 18 mg a.i./L for 7 days, respectively. The biological data showed a significant reduction in survival and body mass in S. eridania and cytotoxic effects in the parietal and perivisceral fat bodies in both species. Ultrastructural cell damage was observed in the trophocytes of both species such as dilated cisternae of the rough endoplasmic reticulum and swollen mitochondria. Trophocytes of S. eridania and C. claveri of the parietal and perivisceral layers responded to those injuries by different cytoprotective and detoxification means such as an increase in the amount of cytoplasmic granules containing calcium, expression of heat shock protein (HSP)70/HSP90, and development of the smooth endoplasmic reticulum. Despite all the different means of cytoprotection and detoxification, they were not sufficient to recover from all the cellular damages. Azadirachtin exhibited an excellent performance for the control of S. eridania and a moderate selectivity for the predator C. claveri, which presents better biological and cytoprotective responses to chronic exposure to azadirachtin. |
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Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposureBiopesticideCytotoxicityInsectMorphologyUltrastructureThe effects of biopesticides on insects can be demonstrated by morphological and ultrastructural tools in ecotoxicological analysis. Azadirachtin-based products are widely used as biopesticides, affecting numerous insect populations. Through morphological biomarkers, this study aimed to characterize the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri after chronic exposure to azadirachtin. Larvae of S. eridania and C. claveri were fed with fresh purple lettuce leaves (Lactuca sativa) and egg clusters of Diatraea saccharalis treated with azadirachtin solution of 6 mg active ingredient (a.i.)/L and 18 mg a.i./L for 7 days, respectively. The biological data showed a significant reduction in survival and body mass in S. eridania and cytotoxic effects in the parietal and perivisceral fat bodies in both species. Ultrastructural cell damage was observed in the trophocytes of both species such as dilated cisternae of the rough endoplasmic reticulum and swollen mitochondria. Trophocytes of S. eridania and C. claveri of the parietal and perivisceral layers responded to those injuries by different cytoprotective and detoxification means such as an increase in the amount of cytoplasmic granules containing calcium, expression of heat shock protein (HSP)70/HSP90, and development of the smooth endoplasmic reticulum. Despite all the different means of cytoprotection and detoxification, they were not sufficient to recover from all the cellular damages. Azadirachtin exhibited an excellent performance for the control of S. eridania and a moderate selectivity for the predator C. claveri, which presents better biological and cytoprotective responses to chronic exposure to azadirachtin.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Insects Department of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Biostatistics Institute of Biosciences of Botucatu São Paulo State University (UNESP)Electron Microscopy Center Institute of Biosciences of Botucatu São Paulo State University (UNESP)Laboratory of Insects Department of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Biostatistics Institute of Biosciences of Botucatu São Paulo State University (UNESP)Electron Microscopy Center Institute of Biosciences of Botucatu São Paulo State University (UNESP)FAPESP: FAPESP 2014/15016-2Universidade Estadual Paulista (Unesp)Scudeler, Elton Luiz [UNESP]Garcia, Ana Silvia Gimenes [UNESP]Padovani, Carlos Roberto [UNESP]dos Santos, Daniela Carvalho [UNESP]2019-10-06T16:14:03Z2019-10-06T16:14:03Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article839-856http://dx.doi.org/10.1007/s00709-019-01347-5Protoplasma, v. 256, n. 3, p. 839-856, 2019.0033-183Xhttp://hdl.handle.net/11449/18862510.1007/s00709-019-01347-52-s2.0-85060085795Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProtoplasmainfo:eu-repo/semantics/openAccess2021-10-23T18:56:51Zoai:repositorio.unesp.br:11449/188625Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:56:56.142454Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposure |
title |
Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposure |
spellingShingle |
Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposure Scudeler, Elton Luiz [UNESP] Biopesticide Cytotoxicity Insect Morphology Ultrastructure |
title_short |
Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposure |
title_full |
Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposure |
title_fullStr |
Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposure |
title_full_unstemmed |
Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposure |
title_sort |
Pest and natural enemy: how the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri react to azadirachtin exposure |
author |
Scudeler, Elton Luiz [UNESP] |
author_facet |
Scudeler, Elton Luiz [UNESP] Garcia, Ana Silvia Gimenes [UNESP] Padovani, Carlos Roberto [UNESP] dos Santos, Daniela Carvalho [UNESP] |
author_role |
author |
author2 |
Garcia, Ana Silvia Gimenes [UNESP] Padovani, Carlos Roberto [UNESP] dos Santos, Daniela Carvalho [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Scudeler, Elton Luiz [UNESP] Garcia, Ana Silvia Gimenes [UNESP] Padovani, Carlos Roberto [UNESP] dos Santos, Daniela Carvalho [UNESP] |
dc.subject.por.fl_str_mv |
Biopesticide Cytotoxicity Insect Morphology Ultrastructure |
topic |
Biopesticide Cytotoxicity Insect Morphology Ultrastructure |
description |
The effects of biopesticides on insects can be demonstrated by morphological and ultrastructural tools in ecotoxicological analysis. Azadirachtin-based products are widely used as biopesticides, affecting numerous insect populations. Through morphological biomarkers, this study aimed to characterize the fat bodies of both the southern armyworm Spodoptera eridania and the predator Ceraeochrysa claveri after chronic exposure to azadirachtin. Larvae of S. eridania and C. claveri were fed with fresh purple lettuce leaves (Lactuca sativa) and egg clusters of Diatraea saccharalis treated with azadirachtin solution of 6 mg active ingredient (a.i.)/L and 18 mg a.i./L for 7 days, respectively. The biological data showed a significant reduction in survival and body mass in S. eridania and cytotoxic effects in the parietal and perivisceral fat bodies in both species. Ultrastructural cell damage was observed in the trophocytes of both species such as dilated cisternae of the rough endoplasmic reticulum and swollen mitochondria. Trophocytes of S. eridania and C. claveri of the parietal and perivisceral layers responded to those injuries by different cytoprotective and detoxification means such as an increase in the amount of cytoplasmic granules containing calcium, expression of heat shock protein (HSP)70/HSP90, and development of the smooth endoplasmic reticulum. Despite all the different means of cytoprotection and detoxification, they were not sufficient to recover from all the cellular damages. Azadirachtin exhibited an excellent performance for the control of S. eridania and a moderate selectivity for the predator C. claveri, which presents better biological and cytoprotective responses to chronic exposure to azadirachtin. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:14:03Z 2019-10-06T16:14:03Z 2019-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00709-019-01347-5 Protoplasma, v. 256, n. 3, p. 839-856, 2019. 0033-183X http://hdl.handle.net/11449/188625 10.1007/s00709-019-01347-5 2-s2.0-85060085795 |
url |
http://dx.doi.org/10.1007/s00709-019-01347-5 http://hdl.handle.net/11449/188625 |
identifier_str_mv |
Protoplasma, v. 256, n. 3, p. 839-856, 2019. 0033-183X 10.1007/s00709-019-01347-5 2-s2.0-85060085795 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Protoplasma |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
839-856 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128293545705473 |