Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)

Detalhes bibliográficos
Autor(a) principal: Campos, V. [UNESP]
Data de Publicação: 2021
Outros Autores: Kaspary, R. M. [UNESP], Rossi, M. J., Medeiros, T. A.M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.15255/CABEQ.2021.1921
http://hdl.handle.net/11449/222846
Resumo: Gamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L-1 h-1 and a yield of 0.4 g g-1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L-1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L-1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation.
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spelling Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)B. licheniformisBiosynthesisSmFSSFγ-polyglutamic acidGamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L-1 h-1 and a yield of 0.4 g g-1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L-1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L-1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (Unesp) Institute of Science and Technology, SPFederal University of Santa Catarina Department of Microbiology Immunology and Parasitology, SCSão Paulo State University (Unesp) Institute of Science and Technology, SPCNPq: 400040/2016-6Universidade Estadual Paulista (UNESP)Universidade Federal de Santa Catarina (UFSC)Campos, V. [UNESP]Kaspary, R. M. [UNESP]Rossi, M. J.Medeiros, T. A.M. [UNESP]2022-04-28T19:47:08Z2022-04-28T19:47:08Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article333-344http://dx.doi.org/10.15255/CABEQ.2021.1921Chemical and Biochemical Engineering Quarterly, v. 35, n. 3, p. 333-344, 2021.1846-51530352-9568http://hdl.handle.net/11449/22284610.15255/CABEQ.2021.19212-s2.0-85118936109Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemical and Biochemical Engineering Quarterlyinfo:eu-repo/semantics/openAccess2022-04-28T19:47:08Zoai:repositorio.unesp.br:11449/222846Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:58:04.488436Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)
title Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)
spellingShingle Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)
Campos, V. [UNESP]
B. licheniformis
Biosynthesis
SmF
SSF
γ-polyglutamic acid
title_short Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)
title_full Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)
title_fullStr Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)
title_full_unstemmed Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)
title_sort Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)
author Campos, V. [UNESP]
author_facet Campos, V. [UNESP]
Kaspary, R. M. [UNESP]
Rossi, M. J.
Medeiros, T. A.M. [UNESP]
author_role author
author2 Kaspary, R. M. [UNESP]
Rossi, M. J.
Medeiros, T. A.M. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de Santa Catarina (UFSC)
dc.contributor.author.fl_str_mv Campos, V. [UNESP]
Kaspary, R. M. [UNESP]
Rossi, M. J.
Medeiros, T. A.M. [UNESP]
dc.subject.por.fl_str_mv B. licheniformis
Biosynthesis
SmF
SSF
γ-polyglutamic acid
topic B. licheniformis
Biosynthesis
SmF
SSF
γ-polyglutamic acid
description Gamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L-1 h-1 and a yield of 0.4 g g-1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L-1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L-1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-01
2022-04-28T19:47:08Z
2022-04-28T19:47:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.15255/CABEQ.2021.1921
Chemical and Biochemical Engineering Quarterly, v. 35, n. 3, p. 333-344, 2021.
1846-5153
0352-9568
http://hdl.handle.net/11449/222846
10.15255/CABEQ.2021.1921
2-s2.0-85118936109
url http://dx.doi.org/10.15255/CABEQ.2021.1921
http://hdl.handle.net/11449/222846
identifier_str_mv Chemical and Biochemical Engineering Quarterly, v. 35, n. 3, p. 333-344, 2021.
1846-5153
0352-9568
10.15255/CABEQ.2021.1921
2-s2.0-85118936109
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemical and Biochemical Engineering Quarterly
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 333-344
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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