Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.15255/CABEQ.2021.1921 http://hdl.handle.net/11449/222846 |
Resumo: | Gamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L-1 h-1 and a yield of 0.4 g g-1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L-1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L-1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation. |
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Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF)B. licheniformisBiosynthesisSmFSSFγ-polyglutamic acidGamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L-1 h-1 and a yield of 0.4 g g-1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L-1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L-1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (Unesp) Institute of Science and Technology, SPFederal University of Santa Catarina Department of Microbiology Immunology and Parasitology, SCSão Paulo State University (Unesp) Institute of Science and Technology, SPCNPq: 400040/2016-6Universidade Estadual Paulista (UNESP)Universidade Federal de Santa Catarina (UFSC)Campos, V. [UNESP]Kaspary, R. M. [UNESP]Rossi, M. J.Medeiros, T. A.M. [UNESP]2022-04-28T19:47:08Z2022-04-28T19:47:08Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article333-344http://dx.doi.org/10.15255/CABEQ.2021.1921Chemical and Biochemical Engineering Quarterly, v. 35, n. 3, p. 333-344, 2021.1846-51530352-9568http://hdl.handle.net/11449/22284610.15255/CABEQ.2021.19212-s2.0-85118936109Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemical and Biochemical Engineering Quarterlyinfo:eu-repo/semantics/openAccess2022-04-28T19:47:08Zoai:repositorio.unesp.br:11449/222846Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:58:04.488436Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF) |
title |
Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF) |
spellingShingle |
Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF) Campos, V. [UNESP] B. licheniformis Biosynthesis SmF SSF γ-polyglutamic acid |
title_short |
Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF) |
title_full |
Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF) |
title_fullStr |
Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF) |
title_full_unstemmed |
Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF) |
title_sort |
Biosynthesis of γ-polyglutamic acid by bacillus licheniformis through submerged fermentation (SmF) and solid-state fermentation (SSF) |
author |
Campos, V. [UNESP] |
author_facet |
Campos, V. [UNESP] Kaspary, R. M. [UNESP] Rossi, M. J. Medeiros, T. A.M. [UNESP] |
author_role |
author |
author2 |
Kaspary, R. M. [UNESP] Rossi, M. J. Medeiros, T. A.M. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de Santa Catarina (UFSC) |
dc.contributor.author.fl_str_mv |
Campos, V. [UNESP] Kaspary, R. M. [UNESP] Rossi, M. J. Medeiros, T. A.M. [UNESP] |
dc.subject.por.fl_str_mv |
B. licheniformis Biosynthesis SmF SSF γ-polyglutamic acid |
topic |
B. licheniformis Biosynthesis SmF SSF γ-polyglutamic acid |
description |
Gamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L-1 h-1 and a yield of 0.4 g g-1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L-1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L-1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-04-28T19:47:08Z 2022-04-28T19:47:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.15255/CABEQ.2021.1921 Chemical and Biochemical Engineering Quarterly, v. 35, n. 3, p. 333-344, 2021. 1846-5153 0352-9568 http://hdl.handle.net/11449/222846 10.15255/CABEQ.2021.1921 2-s2.0-85118936109 |
url |
http://dx.doi.org/10.15255/CABEQ.2021.1921 http://hdl.handle.net/11449/222846 |
identifier_str_mv |
Chemical and Biochemical Engineering Quarterly, v. 35, n. 3, p. 333-344, 2021. 1846-5153 0352-9568 10.15255/CABEQ.2021.1921 2-s2.0-85118936109 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemical and Biochemical Engineering Quarterly |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
333-344 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129269652520960 |