Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1472-6882-12-107 http://hdl.handle.net/11449/7269 |
Resumo: | Background: Ribosome-inactivating proteins (RIP) have been studied in the search for toxins that could be used as immunotoxins for cancer treatment. Pulchellin, a type 2 RIP, is suggested to induce immune responses that have a role in controlling cancer.Methods: The percentage of dendritic cells and CD4(+) and CD8(+) T cells in the spleen (flow cytometry), cytokines' release by PECs and splenocytes (ELISA) and nitric oxide production by PECs (Griess assay) were determined from tumor-bearing mice injected intratumorally with 0.1 ml of pulchellin at 0.75 mu g/kg of body weight. Statistical analysis was performed by one-way ANOVA with Tukey's post hoc test.Results: Pulchellin-treated mice showed significant immune system activation, characterized by increased release of IFN-gamma and Th2 cytokines (IL-4 and IL-10), while IL-6 and TGF-beta levels were decreased. There was also an increase in macrophage's activation, as denoted by the higher percentage of macrophages expressing adhesion and costimulatory molecules (CD54 and CD80, respectively).Conclusions: Our results suggest that pulchellin is promising as an adjuvant in breast cancer treatment. |
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Repositório Institucional da UNESP |
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2946 |
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Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatmentPulchellinBreast cancerCytokinesImmune systemBackground: Ribosome-inactivating proteins (RIP) have been studied in the search for toxins that could be used as immunotoxins for cancer treatment. Pulchellin, a type 2 RIP, is suggested to induce immune responses that have a role in controlling cancer.Methods: The percentage of dendritic cells and CD4(+) and CD8(+) T cells in the spleen (flow cytometry), cytokines' release by PECs and splenocytes (ELISA) and nitric oxide production by PECs (Griess assay) were determined from tumor-bearing mice injected intratumorally with 0.1 ml of pulchellin at 0.75 mu g/kg of body weight. Statistical analysis was performed by one-way ANOVA with Tukey's post hoc test.Results: Pulchellin-treated mice showed significant immune system activation, characterized by increased release of IFN-gamma and Th2 cytokines (IL-4 and IL-10), while IL-6 and TGF-beta levels were decreased. There was also an increase in macrophage's activation, as denoted by the higher percentage of macrophages expressing adhesion and costimulatory molecules (CD54 and CD80, respectively).Conclusions: Our results suggest that pulchellin is promising as an adjuvant in breast cancer treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State Univ, Clin Immunol Lab, Sch Pharmaceut Sci, São Paulo, BrazilUniv Buenos Aires, Inst Oncol Angel H Roffo, Buenos Aires, DF, ArgentinaUniv Estadual Paulista, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, BrazilSão Paulo State Univ, Clin Immunol Lab, Sch Pharmaceut Sci, São Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, BrazilBiomed Central Ltd.Universidade Estadual Paulista (Unesp)Univ Buenos Airesde Matos, Djamile Cordeiro [UNESP]Abreu de Ribeiro, Livia Carolina [UNESP]Tansini, Aline [UNESP]Ferreira, Lucas Souza [UNESP]Polesi Placeres, Marisa Campos [UNESP]Luis Colombo, LucasCarlos, Iracilda Zeppone [UNESP]2014-05-20T13:23:52Z2014-05-20T13:23:52Z2012-07-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://dx.doi.org/10.1186/1472-6882-12-107Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 6, 2012.1472-6882http://hdl.handle.net/11449/726910.1186/1472-6882-12-107WOS:000310323800001WOS000310323800001.pdf1730146818754269Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Complementary and Alternative Medicine2.1090,858info:eu-repo/semantics/openAccess2024-06-21T15:18:45Zoai:repositorio.unesp.br:11449/7269Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:52:25.078032Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment |
title |
Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment |
spellingShingle |
Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment de Matos, Djamile Cordeiro [UNESP] Pulchellin Breast cancer Cytokines Immune system |
title_short |
Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment |
title_full |
Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment |
title_fullStr |
Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment |
title_full_unstemmed |
Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment |
title_sort |
Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment |
author |
de Matos, Djamile Cordeiro [UNESP] |
author_facet |
de Matos, Djamile Cordeiro [UNESP] Abreu de Ribeiro, Livia Carolina [UNESP] Tansini, Aline [UNESP] Ferreira, Lucas Souza [UNESP] Polesi Placeres, Marisa Campos [UNESP] Luis Colombo, Lucas Carlos, Iracilda Zeppone [UNESP] |
author_role |
author |
author2 |
Abreu de Ribeiro, Livia Carolina [UNESP] Tansini, Aline [UNESP] Ferreira, Lucas Souza [UNESP] Polesi Placeres, Marisa Campos [UNESP] Luis Colombo, Lucas Carlos, Iracilda Zeppone [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Buenos Aires |
dc.contributor.author.fl_str_mv |
de Matos, Djamile Cordeiro [UNESP] Abreu de Ribeiro, Livia Carolina [UNESP] Tansini, Aline [UNESP] Ferreira, Lucas Souza [UNESP] Polesi Placeres, Marisa Campos [UNESP] Luis Colombo, Lucas Carlos, Iracilda Zeppone [UNESP] |
dc.subject.por.fl_str_mv |
Pulchellin Breast cancer Cytokines Immune system |
topic |
Pulchellin Breast cancer Cytokines Immune system |
description |
Background: Ribosome-inactivating proteins (RIP) have been studied in the search for toxins that could be used as immunotoxins for cancer treatment. Pulchellin, a type 2 RIP, is suggested to induce immune responses that have a role in controlling cancer.Methods: The percentage of dendritic cells and CD4(+) and CD8(+) T cells in the spleen (flow cytometry), cytokines' release by PECs and splenocytes (ELISA) and nitric oxide production by PECs (Griess assay) were determined from tumor-bearing mice injected intratumorally with 0.1 ml of pulchellin at 0.75 mu g/kg of body weight. Statistical analysis was performed by one-way ANOVA with Tukey's post hoc test.Results: Pulchellin-treated mice showed significant immune system activation, characterized by increased release of IFN-gamma and Th2 cytokines (IL-4 and IL-10), while IL-6 and TGF-beta levels were decreased. There was also an increase in macrophage's activation, as denoted by the higher percentage of macrophages expressing adhesion and costimulatory molecules (CD54 and CD80, respectively).Conclusions: Our results suggest that pulchellin is promising as an adjuvant in breast cancer treatment. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-07-24 2014-05-20T13:23:52Z 2014-05-20T13:23:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1472-6882-12-107 Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 6, 2012. 1472-6882 http://hdl.handle.net/11449/7269 10.1186/1472-6882-12-107 WOS:000310323800001 WOS000310323800001.pdf 1730146818754269 |
url |
http://dx.doi.org/10.1186/1472-6882-12-107 http://hdl.handle.net/11449/7269 |
identifier_str_mv |
Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 6, 2012. 1472-6882 10.1186/1472-6882-12-107 WOS:000310323800001 WOS000310323800001.pdf 1730146818754269 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Complementary and Alternative Medicine 2.109 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128714318282752 |