Human follicle stimulating hormone (hFSH) and thyroxine (T4) in survival maintenance and in vitro growth promotion of caprine preantral follicles

• Autor(a) principal: Costa, Sanely Lourenço da
• Data de Publicação: 2015
• Outros Autores: Costa, Eduardo Paulino da, Pereira, Emílio César Martins [UNESP], Gonçalves, Wagner Gonzaga, Silva, Talita Fernandes da, Queiroz, Vanessa Lopes Dias
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1809-68912015000200298&lng=en&nrm=iso&tlng=en; http://hdl.handle.net/11449/127404
• topic: Activation; Caprine; hFSH; Preantral follicles; T4; Ativação; Caprino; Folículos pré-antrais; hFSH; T4

The aim of this study was to investigate the interaction of human FSH (10ng/ml) with T4 (20ng/mL) on survival, activation and growth of preantral follicles cultured in vitro for 28 days. Fragments of non-cultured and cultured ovarian tissue were processed for classic histology and transmission electron microscopy. The results showed a reduction in the survival rate in all the media tested (one to 28 days) when compared to the fresh control. However the treatment with T4/hFSH for seven days of culture maintained the rate similar to the control. The media tested by one and 28 days reduced the percentage of primordial follicles in all periods of culture. However, T4/hFSH on day one of culture remained similar to the fresh control. None of the media were able to keep the percentage of the developing follicles. It was observed that the follicular diameter in the medium with T4/hFSH remained similar to the fresh control. The ultrastructural analysis confirmed the integrity of follicles cultured for seven days in a medium supplemented with T4/hFSH. In conclusion, the medium with T4/hFSH is able to maintain the survival, promote the activation, and the ultrastructural integrity of caprine preantral follicles for until seven days.