Short communication: JY-1 gene variants and embryo production in Holstein cows
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2020.104308 http://hdl.handle.net/11449/208162 |
Resumo: | The aims of this study were to verify the occurrence of single nucleotide polymorphisms (SNPs) in the JY-1 gene and their association with the in vivo and in vitro production of embryos in Holstein donors. The JY-1 protein is oocyte-specific and has an important regulatory role in folliculogenesis, oocyte maturation, and early embryonic development. Retrospective data of in vivo (n = 572) and in vitro (n = 1068) embryos produced between 2013 and 2015 were obtained from 144 non-lactating, Holstein cows. To identify the polymorphisms, exons 2 and 3 were studied by polymerase chain reaction (PCR) sequencing. The association of SNPs with embryo traits (number of transferable embryos produced in vivo, rate of transferable embryos in vivo, number of embryos produced in vitro, cleavage rate, and embryo rate in vitro) was determined using the GLIMMIX procedure of SAS. Eleven SNPs were detected, located in exon 2, intron 2, and exon 3. The coding genetic variants were non-synonymous, synonymous, and located in untranslated regions. Nine SNPs were associated with at least one in vitro embryo production trait, but none were associated with in vivo embryo production. JY-1 has potential markers that could be used to select donors, and its influence on in vitro embryo production was observed in the present study. |
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Short communication: JY-1 gene variants and embryo production in Holstein cowsBos taurusDonorIn vitro embryoIn vivo embryoSNPThe aims of this study were to verify the occurrence of single nucleotide polymorphisms (SNPs) in the JY-1 gene and their association with the in vivo and in vitro production of embryos in Holstein donors. The JY-1 protein is oocyte-specific and has an important regulatory role in folliculogenesis, oocyte maturation, and early embryonic development. Retrospective data of in vivo (n = 572) and in vitro (n = 1068) embryos produced between 2013 and 2015 were obtained from 144 non-lactating, Holstein cows. To identify the polymorphisms, exons 2 and 3 were studied by polymerase chain reaction (PCR) sequencing. The association of SNPs with embryo traits (number of transferable embryos produced in vivo, rate of transferable embryos in vivo, number of embryos produced in vitro, cleavage rate, and embryo rate in vitro) was determined using the GLIMMIX procedure of SAS. Eleven SNPs were detected, located in exon 2, intron 2, and exon 3. The coding genetic variants were non-synonymous, synonymous, and located in untranslated regions. Nine SNPs were associated with at least one in vitro embryo production trait, but none were associated with in vivo embryo production. JY-1 has potential markers that could be used to select donors, and its influence on in vitro embryo production was observed in the present study.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Pathology Theriogenology and One Health School of Agricultural and Veterinarian Science São Paulo State University (UNESP)Department of Animal Science School of Agricultural and Veterinarian Science São Paulo State University (UNESP)Clínica Veterinária Samvet São CarlosNational Council for Science and Technological DevelopmentDepartment of Pathology Theriogenology and One Health School of Agricultural and Veterinarian Science São Paulo State University (UNESP)Department of Animal Science School of Agricultural and Veterinarian Science São Paulo State University (UNESP)CNPq: 447172/ 2014–0Universidade Estadual Paulista (Unesp)Clínica Veterinária Samvet São CarlosNational Council for Science and Technological DevelopmentSilveira, Cecília R.A. [UNESP]Pierucci, Julia C. [UNESP]de Camargo, Gregório M.F. [UNESP]Barbero, Marina M.D. [UNESP]Cardoso, Diercles F. [UNESP]dos Santos, Daniel J.A. [UNESP]Costa, Raphael B. [UNESP]Tonhati, Humberto [UNESP]de Albuquerque, Lucia G. [UNESP]Rodrigues, Carlos A.Gimenes, Lindsay U. [UNESP]2021-06-25T11:07:26Z2021-06-25T11:07:26Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.livsci.2020.104308Livestock Science, v. 244.1871-1413http://hdl.handle.net/11449/20816210.1016/j.livsci.2020.1043082-s2.0-85096386731Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Scienceinfo:eu-repo/semantics/openAccess2024-06-07T18:45:08Zoai:repositorio.unesp.br:11449/208162Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:00:00.345294Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Short communication: JY-1 gene variants and embryo production in Holstein cows |
title |
Short communication: JY-1 gene variants and embryo production in Holstein cows |
spellingShingle |
Short communication: JY-1 gene variants and embryo production in Holstein cows Silveira, Cecília R.A. [UNESP] Bos taurus Donor In vitro embryo In vivo embryo SNP |
title_short |
Short communication: JY-1 gene variants and embryo production in Holstein cows |
title_full |
Short communication: JY-1 gene variants and embryo production in Holstein cows |
title_fullStr |
Short communication: JY-1 gene variants and embryo production in Holstein cows |
title_full_unstemmed |
Short communication: JY-1 gene variants and embryo production in Holstein cows |
title_sort |
Short communication: JY-1 gene variants and embryo production in Holstein cows |
author |
Silveira, Cecília R.A. [UNESP] |
author_facet |
Silveira, Cecília R.A. [UNESP] Pierucci, Julia C. [UNESP] de Camargo, Gregório M.F. [UNESP] Barbero, Marina M.D. [UNESP] Cardoso, Diercles F. [UNESP] dos Santos, Daniel J.A. [UNESP] Costa, Raphael B. [UNESP] Tonhati, Humberto [UNESP] de Albuquerque, Lucia G. [UNESP] Rodrigues, Carlos A. Gimenes, Lindsay U. [UNESP] |
author_role |
author |
author2 |
Pierucci, Julia C. [UNESP] de Camargo, Gregório M.F. [UNESP] Barbero, Marina M.D. [UNESP] Cardoso, Diercles F. [UNESP] dos Santos, Daniel J.A. [UNESP] Costa, Raphael B. [UNESP] Tonhati, Humberto [UNESP] de Albuquerque, Lucia G. [UNESP] Rodrigues, Carlos A. Gimenes, Lindsay U. [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Clínica Veterinária Samvet São Carlos National Council for Science and Technological Development |
dc.contributor.author.fl_str_mv |
Silveira, Cecília R.A. [UNESP] Pierucci, Julia C. [UNESP] de Camargo, Gregório M.F. [UNESP] Barbero, Marina M.D. [UNESP] Cardoso, Diercles F. [UNESP] dos Santos, Daniel J.A. [UNESP] Costa, Raphael B. [UNESP] Tonhati, Humberto [UNESP] de Albuquerque, Lucia G. [UNESP] Rodrigues, Carlos A. Gimenes, Lindsay U. [UNESP] |
dc.subject.por.fl_str_mv |
Bos taurus Donor In vitro embryo In vivo embryo SNP |
topic |
Bos taurus Donor In vitro embryo In vivo embryo SNP |
description |
The aims of this study were to verify the occurrence of single nucleotide polymorphisms (SNPs) in the JY-1 gene and their association with the in vivo and in vitro production of embryos in Holstein donors. The JY-1 protein is oocyte-specific and has an important regulatory role in folliculogenesis, oocyte maturation, and early embryonic development. Retrospective data of in vivo (n = 572) and in vitro (n = 1068) embryos produced between 2013 and 2015 were obtained from 144 non-lactating, Holstein cows. To identify the polymorphisms, exons 2 and 3 were studied by polymerase chain reaction (PCR) sequencing. The association of SNPs with embryo traits (number of transferable embryos produced in vivo, rate of transferable embryos in vivo, number of embryos produced in vitro, cleavage rate, and embryo rate in vitro) was determined using the GLIMMIX procedure of SAS. Eleven SNPs were detected, located in exon 2, intron 2, and exon 3. The coding genetic variants were non-synonymous, synonymous, and located in untranslated regions. Nine SNPs were associated with at least one in vitro embryo production trait, but none were associated with in vivo embryo production. JY-1 has potential markers that could be used to select donors, and its influence on in vitro embryo production was observed in the present study. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:07:26Z 2021-06-25T11:07:26Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2020.104308 Livestock Science, v. 244. 1871-1413 http://hdl.handle.net/11449/208162 10.1016/j.livsci.2020.104308 2-s2.0-85096386731 |
url |
http://dx.doi.org/10.1016/j.livsci.2020.104308 http://hdl.handle.net/11449/208162 |
identifier_str_mv |
Livestock Science, v. 244. 1871-1413 10.1016/j.livsci.2020.104308 2-s2.0-85096386731 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129570859122688 |