Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice

Detalhes bibliográficos
Autor(a) principal: Martins, M. R. F. B. [UNESP]
Data de Publicação: 2012
Outros Autores: Silva, P. J. R. [UNESP], Martins, B. B., Zoca, S. M., Freitas, M. A., Batista, T. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4067/S0717-95022012000200022
http://hdl.handle.net/11449/16920
Resumo: In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40,50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminifcrous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and leptotene was observed, whereas in the adluminal compartment Golgi phase spermatids we observed the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In Group III, 60 days old, it was found that seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. Therefore morphological evolution of germ cell testicular spermatids can be checked and recognized in its four phases: Golgi, cap, acrosome and maturation over the age of the animal.
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spelling Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni miceAspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/UniSpermatogenesisMouseTestesIn order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40,50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminifcrous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and leptotene was observed, whereas in the adluminal compartment Golgi phase spermatids we observed the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In Group III, 60 days old, it was found that seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. Therefore morphological evolution of germ cell testicular spermatids can be checked and recognized in its four phases: Golgi, cap, acrosome and maturation over the age of the animal.Con el fin de estudiar los cambios morfológicos que ocurren en las células de los testículos del ratón negro isogénico C57BL/6/Uni en tres períodos durante el proceso espermatogenético, se utilizaron 15 ratones divididos en 3 grupos de 5 animales con 40, 50 y 60 días de edad. Los ratones fueron sacrificados y pesados. Luego se pesaron y midieron los testículos, para ser finalmete procesados histológicamente y teñidos con HE, PAS y tricrómico Massom-H y evaluados bajo microscopía de luz. Se observó en el grupo I con 40 días de edad que los túbulos seminíferos tenían un lumen pequeño con escaza cantidad de células tubulares intersticiales. En el epitelio seminífero se identificaron espermatogonias tipo A, intermedio y B, que ocuparon el compartimiento adbasal y entremezcladas con estas células, se observó los espermatocitos I en paquiteno y leptoteno, mientras que en el compartimiento adluminal se observaron espermátidas de fase Golgi en presencia de gránulos acrosomales. En el grupo II, las células del epitelio seminífero se desarrollaron y fue posible observar espermátidas redondas en fase de capuz cefálico además de numerosas espermátidas elongadas en fase acrosómica y células sustentaculares. En el Grupo III, de 60 días de edad, se encontró MARTINS, M. R. F. B.; SILVA, P. J. R.; MARTINS, B. B.; ZOCA, S. M.; FREITAS, M. A. & BATISTA, T. C. Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice. Int. J. Morphol., 30(2):493-498, 2012. 498 que el epitelio seminífero que revestía los túbulos presentaban espermátidas alargadas en fase acrosómica y de maduración, con sus núcleos alargados y el sistema acrosomal típico de la espermiación, con la presencia de espermatozoides y cuerpos residuales cerca del lumen tubular. Así, se puede comprobar con el paso de la edad del animal, la evolución morfológica de las células germinativas testiculares y reconocer las espermátidas en sus cuatro fases: Golgi, capuchón, acrosomal y de maduración.Univ Estadual Paulista, Dept Anat, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Anat, BR-18618970 Botucatu, SP, BrazilSoc Chilena AnatomiaUniversidade Estadual Paulista (Unesp)Martins, M. R. F. B. [UNESP]Silva, P. J. R. [UNESP]Martins, B. B.Zoca, S. M.Freitas, M. A.Batista, T. C.2014-05-20T13:47:33Z2014-05-20T13:47:33Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article493-498application/pdfhttp://dx.doi.org/10.4067/S0717-95022012000200022International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 30, n. 2, p. 493-498, 2012.0717-9502http://hdl.handle.net/11449/16920S0717-95022012000200022WOS:000308261900022WOS:000304785800052S0717-95022012000200022.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Morphology0.3360,207info:eu-repo/semantics/openAccess2023-11-03T06:06:26Zoai:repositorio.unesp.br:11449/16920Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:46:20.509264Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni
title Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice
spellingShingle Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice
Martins, M. R. F. B. [UNESP]
Spermatogenesis
Mouse
Testes
title_short Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice
title_full Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice
title_fullStr Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice
title_full_unstemmed Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice
title_sort Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice
author Martins, M. R. F. B. [UNESP]
author_facet Martins, M. R. F. B. [UNESP]
Silva, P. J. R. [UNESP]
Martins, B. B.
Zoca, S. M.
Freitas, M. A.
Batista, T. C.
author_role author
author2 Silva, P. J. R. [UNESP]
Martins, B. B.
Zoca, S. M.
Freitas, M. A.
Batista, T. C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Martins, M. R. F. B. [UNESP]
Silva, P. J. R. [UNESP]
Martins, B. B.
Zoca, S. M.
Freitas, M. A.
Batista, T. C.
dc.subject.por.fl_str_mv Spermatogenesis
Mouse
Testes
topic Spermatogenesis
Mouse
Testes
description In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40,50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminifcrous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and leptotene was observed, whereas in the adluminal compartment Golgi phase spermatids we observed the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In Group III, 60 days old, it was found that seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. Therefore morphological evolution of germ cell testicular spermatids can be checked and recognized in its four phases: Golgi, cap, acrosome and maturation over the age of the animal.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2014-05-20T13:47:33Z
2014-05-20T13:47:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4067/S0717-95022012000200022
International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 30, n. 2, p. 493-498, 2012.
0717-9502
http://hdl.handle.net/11449/16920
S0717-95022012000200022
WOS:000308261900022
WOS:000304785800052
S0717-95022012000200022.pdf
url http://dx.doi.org/10.4067/S0717-95022012000200022
http://hdl.handle.net/11449/16920
identifier_str_mv International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 30, n. 2, p. 493-498, 2012.
0717-9502
S0717-95022012000200022
WOS:000308261900022
WOS:000304785800052
S0717-95022012000200022.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Morphology
0.336
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 493-498
application/pdf
dc.publisher.none.fl_str_mv Soc Chilena Anatomia
publisher.none.fl_str_mv Soc Chilena Anatomia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
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reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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