Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/ocr.12340 http://hdl.handle.net/11449/201272 |
Resumo: | Objectives: This study evaluated, in experimental model, the inflammatory alterations in gingival tissue and alveolar bone during the orthodontic tooth movement (OTM) in diabetes mellitus (D) and periodontitis (P). Setting and Sample Population: Forty male Wistar rats, 90 days old and weighing 300 g. Materials and Methods: The sample was divided into four groups (n = 10). OTM: orthodontic movement (10 days, 0.4 N force); P + OTM: periodontitis (ligature-induced periodontitis, 3-0 silk suture thread) and orthodontic movement; D + OTM: diabetes (Alloxan-induced diabetes, 150 mg/kg) and orthodontic movement; and D + P + OTM: diabetes, periodontitis and orthodontic movement. Tooth displacement was measured; fibroblast, inflammatory cells, osteoclast and blood vessels were quantified by histomorphometric analysis. Inflammatory markers, interleukin-6 (IL-6) and tumour necrosis factor (TNF-α) were quantified by ELISA (Enzyme-Linked Immunosorbent Assay) in gingival tissue. The fibroblastic growth factor (bFGF), transforming growth factor (TGF-β1) and the vascular endothelial growth factor (VEGF) were measured via Western blotting in the alveolar bone. The results were analysed by ANOVA and Tukey's test at a 5% significance level. Results: The quantification of inflammatory cells and the expression of IL-6, TNF-α, TGF-β1 and bFGF were increased in diabetes and periodontitis. However, the number of fibroblasts and blood vessels and the percentage of birefringent collagen fibres were higher in healthy animals. There was greater tooth displacement in the OTM group. Conclusion: Diabetes Mellitus modifies the inflammatory response. The increased expression of inflammatory markers IL-6, TNF-α and TGF-β1 in diabetic animals impairs neovasculogenesis and tissue reorganization during orthodontic tooth movement, which may be aggravated by periodontitis. |
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Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movementdiabetes mellitusinflammationorthodontic tooth movementperiodontitisObjectives: This study evaluated, in experimental model, the inflammatory alterations in gingival tissue and alveolar bone during the orthodontic tooth movement (OTM) in diabetes mellitus (D) and periodontitis (P). Setting and Sample Population: Forty male Wistar rats, 90 days old and weighing 300 g. Materials and Methods: The sample was divided into four groups (n = 10). OTM: orthodontic movement (10 days, 0.4 N force); P + OTM: periodontitis (ligature-induced periodontitis, 3-0 silk suture thread) and orthodontic movement; D + OTM: diabetes (Alloxan-induced diabetes, 150 mg/kg) and orthodontic movement; and D + P + OTM: diabetes, periodontitis and orthodontic movement. Tooth displacement was measured; fibroblast, inflammatory cells, osteoclast and blood vessels were quantified by histomorphometric analysis. Inflammatory markers, interleukin-6 (IL-6) and tumour necrosis factor (TNF-α) were quantified by ELISA (Enzyme-Linked Immunosorbent Assay) in gingival tissue. The fibroblastic growth factor (bFGF), transforming growth factor (TGF-β1) and the vascular endothelial growth factor (VEGF) were measured via Western blotting in the alveolar bone. The results were analysed by ANOVA and Tukey's test at a 5% significance level. Results: The quantification of inflammatory cells and the expression of IL-6, TNF-α, TGF-β1 and bFGF were increased in diabetes and periodontitis. However, the number of fibroblasts and blood vessels and the percentage of birefringent collagen fibres were higher in healthy animals. There was greater tooth displacement in the OTM group. Conclusion: Diabetes Mellitus modifies the inflammatory response. The increased expression of inflammatory markers IL-6, TNF-α and TGF-β1 in diabetic animals impairs neovasculogenesis and tissue reorganization during orthodontic tooth movement, which may be aggravated by periodontitis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Graduate Program of Biomedical Sciences University Center of the Hermínio Ometto Foundation-FHOGraduate Program of Orthodontics University Center of the Hermínio Ometto Foundation-FHOICT-UNESP Division of Periodontics UNESP São Paulo State UniversityICT-UNESP Division of Periodontics UNESP São Paulo State UniversityCAPES: Process no 23038.008192/2013-01University Center of the Hermínio Ometto Foundation-FHOUniversidade Estadual Paulista (Unesp)Santamaria-Jr, MiltonBagne, LeonardoZaniboni, EwertonSantamaria, Mauro Pedrine [UNESP]Jardini, Maria Aparecida Neves [UNESP]Felonato, Maírados Santos, Gláucia Maria TechMendonça, Fernanda Aparecida SampaioEsquisatto, Marcelo Augusto Marretto2020-12-12T02:28:24Z2020-12-12T02:28:24Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27-34http://dx.doi.org/10.1111/ocr.12340Orthodontics and Craniofacial Research, v. 23, n. 1, p. 27-34, 2020.1601-63431601-6335http://hdl.handle.net/11449/20127210.1111/ocr.123402-s2.0-85073989629Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOrthodontics and Craniofacial Researchinfo:eu-repo/semantics/openAccess2021-10-22T14:02:59Zoai:repositorio.unesp.br:11449/201272Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:21:58.840817Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement |
title |
Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement |
spellingShingle |
Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement Santamaria-Jr, Milton diabetes mellitus inflammation orthodontic tooth movement periodontitis |
title_short |
Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement |
title_full |
Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement |
title_fullStr |
Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement |
title_full_unstemmed |
Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement |
title_sort |
Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement |
author |
Santamaria-Jr, Milton |
author_facet |
Santamaria-Jr, Milton Bagne, Leonardo Zaniboni, Ewerton Santamaria, Mauro Pedrine [UNESP] Jardini, Maria Aparecida Neves [UNESP] Felonato, Maíra dos Santos, Gláucia Maria Tech Mendonça, Fernanda Aparecida Sampaio Esquisatto, Marcelo Augusto Marretto |
author_role |
author |
author2 |
Bagne, Leonardo Zaniboni, Ewerton Santamaria, Mauro Pedrine [UNESP] Jardini, Maria Aparecida Neves [UNESP] Felonato, Maíra dos Santos, Gláucia Maria Tech Mendonça, Fernanda Aparecida Sampaio Esquisatto, Marcelo Augusto Marretto |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
University Center of the Hermínio Ometto Foundation-FHO Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Santamaria-Jr, Milton Bagne, Leonardo Zaniboni, Ewerton Santamaria, Mauro Pedrine [UNESP] Jardini, Maria Aparecida Neves [UNESP] Felonato, Maíra dos Santos, Gláucia Maria Tech Mendonça, Fernanda Aparecida Sampaio Esquisatto, Marcelo Augusto Marretto |
dc.subject.por.fl_str_mv |
diabetes mellitus inflammation orthodontic tooth movement periodontitis |
topic |
diabetes mellitus inflammation orthodontic tooth movement periodontitis |
description |
Objectives: This study evaluated, in experimental model, the inflammatory alterations in gingival tissue and alveolar bone during the orthodontic tooth movement (OTM) in diabetes mellitus (D) and periodontitis (P). Setting and Sample Population: Forty male Wistar rats, 90 days old and weighing 300 g. Materials and Methods: The sample was divided into four groups (n = 10). OTM: orthodontic movement (10 days, 0.4 N force); P + OTM: periodontitis (ligature-induced periodontitis, 3-0 silk suture thread) and orthodontic movement; D + OTM: diabetes (Alloxan-induced diabetes, 150 mg/kg) and orthodontic movement; and D + P + OTM: diabetes, periodontitis and orthodontic movement. Tooth displacement was measured; fibroblast, inflammatory cells, osteoclast and blood vessels were quantified by histomorphometric analysis. Inflammatory markers, interleukin-6 (IL-6) and tumour necrosis factor (TNF-α) were quantified by ELISA (Enzyme-Linked Immunosorbent Assay) in gingival tissue. The fibroblastic growth factor (bFGF), transforming growth factor (TGF-β1) and the vascular endothelial growth factor (VEGF) were measured via Western blotting in the alveolar bone. The results were analysed by ANOVA and Tukey's test at a 5% significance level. Results: The quantification of inflammatory cells and the expression of IL-6, TNF-α, TGF-β1 and bFGF were increased in diabetes and periodontitis. However, the number of fibroblasts and blood vessels and the percentage of birefringent collagen fibres were higher in healthy animals. There was greater tooth displacement in the OTM group. Conclusion: Diabetes Mellitus modifies the inflammatory response. The increased expression of inflammatory markers IL-6, TNF-α and TGF-β1 in diabetic animals impairs neovasculogenesis and tissue reorganization during orthodontic tooth movement, which may be aggravated by periodontitis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:28:24Z 2020-12-12T02:28:24Z 2020-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/ocr.12340 Orthodontics and Craniofacial Research, v. 23, n. 1, p. 27-34, 2020. 1601-6343 1601-6335 http://hdl.handle.net/11449/201272 10.1111/ocr.12340 2-s2.0-85073989629 |
url |
http://dx.doi.org/10.1111/ocr.12340 http://hdl.handle.net/11449/201272 |
identifier_str_mv |
Orthodontics and Craniofacial Research, v. 23, n. 1, p. 27-34, 2020. 1601-6343 1601-6335 10.1111/ocr.12340 2-s2.0-85073989629 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Orthodontics and Craniofacial Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
27-34 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128798856577024 |