Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement

Detalhes bibliográficos
Autor(a) principal: Santamaria-Jr, Milton
Data de Publicação: 2020
Outros Autores: Bagne, Leonardo, Zaniboni, Ewerton, Santamaria, Mauro Pedrine [UNESP], Jardini, Maria Aparecida Neves [UNESP], Felonato, Maíra, dos Santos, Gláucia Maria Tech, Mendonça, Fernanda Aparecida Sampaio, Esquisatto, Marcelo Augusto Marretto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/ocr.12340
http://hdl.handle.net/11449/201272
Resumo: Objectives: This study evaluated, in experimental model, the inflammatory alterations in gingival tissue and alveolar bone during the orthodontic tooth movement (OTM) in diabetes mellitus (D) and periodontitis (P). Setting and Sample Population: Forty male Wistar rats, 90 days old and weighing 300 g. Materials and Methods: The sample was divided into four groups (n = 10). OTM: orthodontic movement (10 days, 0.4 N force); P + OTM: periodontitis (ligature-induced periodontitis, 3-0 silk suture thread) and orthodontic movement; D + OTM: diabetes (Alloxan-induced diabetes, 150 mg/kg) and orthodontic movement; and D + P + OTM: diabetes, periodontitis and orthodontic movement. Tooth displacement was measured; fibroblast, inflammatory cells, osteoclast and blood vessels were quantified by histomorphometric analysis. Inflammatory markers, interleukin-6 (IL-6) and tumour necrosis factor (TNF-α) were quantified by ELISA (Enzyme-Linked Immunosorbent Assay) in gingival tissue. The fibroblastic growth factor (bFGF), transforming growth factor (TGF-β1) and the vascular endothelial growth factor (VEGF) were measured via Western blotting in the alveolar bone. The results were analysed by ANOVA and Tukey's test at a 5% significance level. Results: The quantification of inflammatory cells and the expression of IL-6, TNF-α, TGF-β1 and bFGF were increased in diabetes and periodontitis. However, the number of fibroblasts and blood vessels and the percentage of birefringent collagen fibres were higher in healthy animals. There was greater tooth displacement in the OTM group. Conclusion: Diabetes Mellitus modifies the inflammatory response. The increased expression of inflammatory markers IL-6, TNF-α and TGF-β1 in diabetic animals impairs neovasculogenesis and tissue reorganization during orthodontic tooth movement, which may be aggravated by periodontitis.
id UNSP_6d5ef153d8407f42009bbd204fc3453d
oai_identifier_str oai:repositorio.unesp.br:11449/201272
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movementdiabetes mellitusinflammationorthodontic tooth movementperiodontitisObjectives: This study evaluated, in experimental model, the inflammatory alterations in gingival tissue and alveolar bone during the orthodontic tooth movement (OTM) in diabetes mellitus (D) and periodontitis (P). Setting and Sample Population: Forty male Wistar rats, 90 days old and weighing 300 g. Materials and Methods: The sample was divided into four groups (n = 10). OTM: orthodontic movement (10 days, 0.4 N force); P + OTM: periodontitis (ligature-induced periodontitis, 3-0 silk suture thread) and orthodontic movement; D + OTM: diabetes (Alloxan-induced diabetes, 150 mg/kg) and orthodontic movement; and D + P + OTM: diabetes, periodontitis and orthodontic movement. Tooth displacement was measured; fibroblast, inflammatory cells, osteoclast and blood vessels were quantified by histomorphometric analysis. Inflammatory markers, interleukin-6 (IL-6) and tumour necrosis factor (TNF-α) were quantified by ELISA (Enzyme-Linked Immunosorbent Assay) in gingival tissue. The fibroblastic growth factor (bFGF), transforming growth factor (TGF-β1) and the vascular endothelial growth factor (VEGF) were measured via Western blotting in the alveolar bone. The results were analysed by ANOVA and Tukey's test at a 5% significance level. Results: The quantification of inflammatory cells and the expression of IL-6, TNF-α, TGF-β1 and bFGF were increased in diabetes and periodontitis. However, the number of fibroblasts and blood vessels and the percentage of birefringent collagen fibres were higher in healthy animals. There was greater tooth displacement in the OTM group. Conclusion: Diabetes Mellitus modifies the inflammatory response. The increased expression of inflammatory markers IL-6, TNF-α and TGF-β1 in diabetic animals impairs neovasculogenesis and tissue reorganization during orthodontic tooth movement, which may be aggravated by periodontitis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Graduate Program of Biomedical Sciences University Center of the Hermínio Ometto Foundation-FHOGraduate Program of Orthodontics University Center of the Hermínio Ometto Foundation-FHOICT-UNESP Division of Periodontics UNESP São Paulo State UniversityICT-UNESP Division of Periodontics UNESP São Paulo State UniversityCAPES: Process no 23038.008192/2013-01University Center of the Hermínio Ometto Foundation-FHOUniversidade Estadual Paulista (Unesp)Santamaria-Jr, MiltonBagne, LeonardoZaniboni, EwertonSantamaria, Mauro Pedrine [UNESP]Jardini, Maria Aparecida Neves [UNESP]Felonato, Maírados Santos, Gláucia Maria TechMendonça, Fernanda Aparecida SampaioEsquisatto, Marcelo Augusto Marretto2020-12-12T02:28:24Z2020-12-12T02:28:24Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27-34http://dx.doi.org/10.1111/ocr.12340Orthodontics and Craniofacial Research, v. 23, n. 1, p. 27-34, 2020.1601-63431601-6335http://hdl.handle.net/11449/20127210.1111/ocr.123402-s2.0-85073989629Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOrthodontics and Craniofacial Researchinfo:eu-repo/semantics/openAccess2021-10-22T14:02:59Zoai:repositorio.unesp.br:11449/201272Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:21:58.840817Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement
title Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement
spellingShingle Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement
Santamaria-Jr, Milton
diabetes mellitus
inflammation
orthodontic tooth movement
periodontitis
title_short Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement
title_full Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement
title_fullStr Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement
title_full_unstemmed Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement
title_sort Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement
author Santamaria-Jr, Milton
author_facet Santamaria-Jr, Milton
Bagne, Leonardo
Zaniboni, Ewerton
Santamaria, Mauro Pedrine [UNESP]
Jardini, Maria Aparecida Neves [UNESP]
Felonato, Maíra
dos Santos, Gláucia Maria Tech
Mendonça, Fernanda Aparecida Sampaio
Esquisatto, Marcelo Augusto Marretto
author_role author
author2 Bagne, Leonardo
Zaniboni, Ewerton
Santamaria, Mauro Pedrine [UNESP]
Jardini, Maria Aparecida Neves [UNESP]
Felonato, Maíra
dos Santos, Gláucia Maria Tech
Mendonça, Fernanda Aparecida Sampaio
Esquisatto, Marcelo Augusto Marretto
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University Center of the Hermínio Ometto Foundation-FHO
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Santamaria-Jr, Milton
Bagne, Leonardo
Zaniboni, Ewerton
Santamaria, Mauro Pedrine [UNESP]
Jardini, Maria Aparecida Neves [UNESP]
Felonato, Maíra
dos Santos, Gláucia Maria Tech
Mendonça, Fernanda Aparecida Sampaio
Esquisatto, Marcelo Augusto Marretto
dc.subject.por.fl_str_mv diabetes mellitus
inflammation
orthodontic tooth movement
periodontitis
topic diabetes mellitus
inflammation
orthodontic tooth movement
periodontitis
description Objectives: This study evaluated, in experimental model, the inflammatory alterations in gingival tissue and alveolar bone during the orthodontic tooth movement (OTM) in diabetes mellitus (D) and periodontitis (P). Setting and Sample Population: Forty male Wistar rats, 90 days old and weighing 300 g. Materials and Methods: The sample was divided into four groups (n = 10). OTM: orthodontic movement (10 days, 0.4 N force); P + OTM: periodontitis (ligature-induced periodontitis, 3-0 silk suture thread) and orthodontic movement; D + OTM: diabetes (Alloxan-induced diabetes, 150 mg/kg) and orthodontic movement; and D + P + OTM: diabetes, periodontitis and orthodontic movement. Tooth displacement was measured; fibroblast, inflammatory cells, osteoclast and blood vessels were quantified by histomorphometric analysis. Inflammatory markers, interleukin-6 (IL-6) and tumour necrosis factor (TNF-α) were quantified by ELISA (Enzyme-Linked Immunosorbent Assay) in gingival tissue. The fibroblastic growth factor (bFGF), transforming growth factor (TGF-β1) and the vascular endothelial growth factor (VEGF) were measured via Western blotting in the alveolar bone. The results were analysed by ANOVA and Tukey's test at a 5% significance level. Results: The quantification of inflammatory cells and the expression of IL-6, TNF-α, TGF-β1 and bFGF were increased in diabetes and periodontitis. However, the number of fibroblasts and blood vessels and the percentage of birefringent collagen fibres were higher in healthy animals. There was greater tooth displacement in the OTM group. Conclusion: Diabetes Mellitus modifies the inflammatory response. The increased expression of inflammatory markers IL-6, TNF-α and TGF-β1 in diabetic animals impairs neovasculogenesis and tissue reorganization during orthodontic tooth movement, which may be aggravated by periodontitis.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:28:24Z
2020-12-12T02:28:24Z
2020-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/ocr.12340
Orthodontics and Craniofacial Research, v. 23, n. 1, p. 27-34, 2020.
1601-6343
1601-6335
http://hdl.handle.net/11449/201272
10.1111/ocr.12340
2-s2.0-85073989629
url http://dx.doi.org/10.1111/ocr.12340
http://hdl.handle.net/11449/201272
identifier_str_mv Orthodontics and Craniofacial Research, v. 23, n. 1, p. 27-34, 2020.
1601-6343
1601-6335
10.1111/ocr.12340
2-s2.0-85073989629
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Orthodontics and Craniofacial Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 27-34
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128798856577024