Leucograma e perfil bioquímico sérico de cabras com mastite experimental causada por Staphylococcus aureus e Streptococcus agalactiae

• Autor(a) principal: Simplicio, Kalina Maria de Medeiros Gomes [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/122070
• topic: Caprino; Cabra; Mastite; Marcadores biologicos; Inflamação; Proteínas; Infecções estreptococicas; Infecções estafilococicas; Inflammation

Acute phase proteins (APP) are sensitive and early indicators of inflammation. However, information regarding goats APP are scarce in the literature. So the aim of this study was to determine the leukocyte count and serum biochemical profile, in particular APP, in goats with induced Staphylococcus aureus (Group I) and Streptococcus agalactiae (Group II) mastitis in order to verify its importance as early biomarkers of mammary infection in these species. GI was composed of five Saanen goats inoculated through the teat canal with about 9.5 x 109 colony forming units (CFU) of S. aureus and GII consisted of five French Alpine goats inoculated with approximately 4.4 x 1011 CFU of S. agalactiae. The experimental moment immediately prior to inoculation was designated M0 and the following identified as M1, M2, M3, M4, M5, M6 and M7 when blood and milk samples were obtained. Leucocyte count and measurement of plasma fibrinogen concentration, serum levels of aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase, creatine kinase, total protein, albumin, urea, creatinine, total calcium, phosphorus, magnesium and iron, were determined with blood samples, using commercial kits. Serum transferrin, ceruloplasmin, haptoglobin and α1-acid glycoprotein were obtained by the SDS-Page Electrophoresis technique. Milk samples were used to monitor the development of clinical mastitis through the strip cup test, California Mastitis Test (CMT) and microbiological analyzes. Data were subjected to analysis of variance and the means to Dunnet test, considering a 95% significance level (p < 0.05). It was noted that the experimental infection model developed for the induction of clinical mastitis in goats was effective in both groups. Leucocyte count and serum biochemical profile, with the exception of APP, were unreliable laboratory tests to aid in the diagnosis or monitoring of experimental mastitis in goats, whether induced by S. aureus or ...