Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission route
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.actatropica.2020.105497 http://hdl.handle.net/11449/201708 |
Resumo: | It is possible that there are peculiarities in the epidemiology of leptospirosis in regions with a semiarid climate, where the environment is often adverse, allowing the occurrence of alternative transmission routes. The objective of the work was to generate contributions to the diagnosis and epidemiology of Leptospira sp. infection in sheep reared in semiarid conditions, using serological, molecular and microbiological techniques for diagnosis in dry and rainy seasons. Samples of blood, vaginal fluid, urine, bladder, kidney, uterus, uterine tube, ovary and placenta were collected from 104 sheep (52 animals per season – dry and rainy) slaughtered in the Brazilian semiarid. Diagnostic tests performed were microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Anti-Leptospira sp. antibodies were found in 26 (25%) of the animals analyzed by MAT at 1:50 dilution (cut-off 50), while 69 (66.3%) animals had at least one organ/fluid with the presence of Leptospira sp. DNA. Overall, PCR was performed on 758 fragments of organs/fluids from the genital and urinary tracts, and 519 (68.5%) samples tested positive. PCR-positivity was statistically different in dry (46.2%) and rainy (11.5%) seasons for vaginal fluid. It was possible to perform the DNA sequencing in nine samples with 99% similarity to L. interrogans and recovery of viable strains in three samples of vaginal fluid. Regardless of the biological material used in PCR to detect carrier animals and the season, the highest MAT sensitivity values were obtained with cut-off 50 compared to 100. The results obtained indicate that, even in the adverse environmental conditions of the semiarid region, leptospires may survive and propagate by alternative routes of transmission, such as sexual, and the presence of PCR-positive genital tracts in ewes suggests that sexual transmission may play an important role in the epidemiology of the disease in sheep in Brazilian semiarid. In addition, it is suggested the use of titer 50 as cut-off point at serology in semiarid conditions. |
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Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission routeCut-off 50Genital leptospirosisPCRSheep leptospirosisVaginal fluidVenereal transmissionIt is possible that there are peculiarities in the epidemiology of leptospirosis in regions with a semiarid climate, where the environment is often adverse, allowing the occurrence of alternative transmission routes. The objective of the work was to generate contributions to the diagnosis and epidemiology of Leptospira sp. infection in sheep reared in semiarid conditions, using serological, molecular and microbiological techniques for diagnosis in dry and rainy seasons. Samples of blood, vaginal fluid, urine, bladder, kidney, uterus, uterine tube, ovary and placenta were collected from 104 sheep (52 animals per season – dry and rainy) slaughtered in the Brazilian semiarid. Diagnostic tests performed were microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Anti-Leptospira sp. antibodies were found in 26 (25%) of the animals analyzed by MAT at 1:50 dilution (cut-off 50), while 69 (66.3%) animals had at least one organ/fluid with the presence of Leptospira sp. DNA. Overall, PCR was performed on 758 fragments of organs/fluids from the genital and urinary tracts, and 519 (68.5%) samples tested positive. PCR-positivity was statistically different in dry (46.2%) and rainy (11.5%) seasons for vaginal fluid. It was possible to perform the DNA sequencing in nine samples with 99% similarity to L. interrogans and recovery of viable strains in three samples of vaginal fluid. Regardless of the biological material used in PCR to detect carrier animals and the season, the highest MAT sensitivity values were obtained with cut-off 50 compared to 100. The results obtained indicate that, even in the adverse environmental conditions of the semiarid region, leptospires may survive and propagate by alternative routes of transmission, such as sexual, and the presence of PCR-positive genital tracts in ewes suggests that sexual transmission may play an important role in the epidemiology of the disease in sheep in Brazilian semiarid. In addition, it is suggested the use of titer 50 as cut-off point at serology in semiarid conditions.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Centro de Saúde e Tecnologia Rural (CSTR) Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa CecíliaDepartamento de Ciências Veterinárias (DCV) Universidade Federal da Paraíba (UFPB), PB-079, s/n, Cidade UniversitáriaUniversidade Estadual Paulista (Unesp), Av. Prof. Mário Rubens Guimarães Montenegro, s/n, campus de BotucatuUniversidade Estadual Paulista (Unesp), Av. Prof. Mário Rubens Guimarães Montenegro, s/n, campus de BotucatuCNPq: 302222/2016-2CNPq: 423836/2018-8Universidade Federal de Campina Grande (UFCG)Universidade Federal da Paraíba (UFPB)Universidade Estadual Paulista (Unesp)Nogueira, Denise Batistada Costa, Flávia Teresa RibeiroBezerra, Camila de SousaSilva, Maria Luana Cristiny Rodriguesda Costa, Diego FigueiredoViana, Maira Portoda Silva, José DêvedeJúnior, João Pessoa Araújo [UNESP]Malossi, Camila Dantas [UNESP]Ullmann, Leila Sabrina [UNESP]Santos, Carolina de Sousa Américo BatistaAlves, Clebert Joséde Azevedo, Sérgio Santos2020-12-12T02:39:42Z2020-12-12T02:39:42Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.actatropica.2020.105497Acta Tropica, v. 207.1873-62540001-706Xhttp://hdl.handle.net/11449/20170810.1016/j.actatropica.2020.1054972-s2.0-85083800868Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2021-10-22T21:03:03Zoai:repositorio.unesp.br:11449/201708Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:03:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission route |
title |
Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission route |
spellingShingle |
Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission route Nogueira, Denise Batista Cut-off 50 Genital leptospirosis PCR Sheep leptospirosis Vaginal fluid Venereal transmission |
title_short |
Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission route |
title_full |
Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission route |
title_fullStr |
Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission route |
title_full_unstemmed |
Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission route |
title_sort |
Use of serological and molecular techniques for detection of Leptospira sp. carrier sheep under semiarid conditions and the importance of genital transmission route |
author |
Nogueira, Denise Batista |
author_facet |
Nogueira, Denise Batista da Costa, Flávia Teresa Ribeiro Bezerra, Camila de Sousa Silva, Maria Luana Cristiny Rodrigues da Costa, Diego Figueiredo Viana, Maira Porto da Silva, José Dêvede Júnior, João Pessoa Araújo [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Santos, Carolina de Sousa Américo Batista Alves, Clebert José de Azevedo, Sérgio Santos |
author_role |
author |
author2 |
da Costa, Flávia Teresa Ribeiro Bezerra, Camila de Sousa Silva, Maria Luana Cristiny Rodrigues da Costa, Diego Figueiredo Viana, Maira Porto da Silva, José Dêvede Júnior, João Pessoa Araújo [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Santos, Carolina de Sousa Américo Batista Alves, Clebert José de Azevedo, Sérgio Santos |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Campina Grande (UFCG) Universidade Federal da Paraíba (UFPB) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nogueira, Denise Batista da Costa, Flávia Teresa Ribeiro Bezerra, Camila de Sousa Silva, Maria Luana Cristiny Rodrigues da Costa, Diego Figueiredo Viana, Maira Porto da Silva, José Dêvede Júnior, João Pessoa Araújo [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Santos, Carolina de Sousa Américo Batista Alves, Clebert José de Azevedo, Sérgio Santos |
dc.subject.por.fl_str_mv |
Cut-off 50 Genital leptospirosis PCR Sheep leptospirosis Vaginal fluid Venereal transmission |
topic |
Cut-off 50 Genital leptospirosis PCR Sheep leptospirosis Vaginal fluid Venereal transmission |
description |
It is possible that there are peculiarities in the epidemiology of leptospirosis in regions with a semiarid climate, where the environment is often adverse, allowing the occurrence of alternative transmission routes. The objective of the work was to generate contributions to the diagnosis and epidemiology of Leptospira sp. infection in sheep reared in semiarid conditions, using serological, molecular and microbiological techniques for diagnosis in dry and rainy seasons. Samples of blood, vaginal fluid, urine, bladder, kidney, uterus, uterine tube, ovary and placenta were collected from 104 sheep (52 animals per season – dry and rainy) slaughtered in the Brazilian semiarid. Diagnostic tests performed were microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Anti-Leptospira sp. antibodies were found in 26 (25%) of the animals analyzed by MAT at 1:50 dilution (cut-off 50), while 69 (66.3%) animals had at least one organ/fluid with the presence of Leptospira sp. DNA. Overall, PCR was performed on 758 fragments of organs/fluids from the genital and urinary tracts, and 519 (68.5%) samples tested positive. PCR-positivity was statistically different in dry (46.2%) and rainy (11.5%) seasons for vaginal fluid. It was possible to perform the DNA sequencing in nine samples with 99% similarity to L. interrogans and recovery of viable strains in three samples of vaginal fluid. Regardless of the biological material used in PCR to detect carrier animals and the season, the highest MAT sensitivity values were obtained with cut-off 50 compared to 100. The results obtained indicate that, even in the adverse environmental conditions of the semiarid region, leptospires may survive and propagate by alternative routes of transmission, such as sexual, and the presence of PCR-positive genital tracts in ewes suggests that sexual transmission may play an important role in the epidemiology of the disease in sheep in Brazilian semiarid. In addition, it is suggested the use of titer 50 as cut-off point at serology in semiarid conditions. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:39:42Z 2020-12-12T02:39:42Z 2020-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2020.105497 Acta Tropica, v. 207. 1873-6254 0001-706X http://hdl.handle.net/11449/201708 10.1016/j.actatropica.2020.105497 2-s2.0-85083800868 |
url |
http://dx.doi.org/10.1016/j.actatropica.2020.105497 http://hdl.handle.net/11449/201708 |
identifier_str_mv |
Acta Tropica, v. 207. 1873-6254 0001-706X 10.1016/j.actatropica.2020.105497 2-s2.0-85083800868 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Tropica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964723808043008 |