Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity

Detalhes bibliográficos
Autor(a) principal: Veríssimo, Nathalia V. [UNESP]
Data de Publicação: 2021
Outros Autores: Saponi, Carolina F. [UNESP], Ryan, Timothy M., Greaves, Tamar L., Pereira, Jorge F.B. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.gce.2021.08.001
http://hdl.handle.net/11449/223399
Resumo: Fluorescent Proteins (FP) can be applied as biomarkers and biosensors in the industrial and medical fields, but their large-scale use, especially for new industrial applications, is limited due to their low stability. Hence, the discovery of additives capable of preserving the activity of FP at room temperature and under stress conditions can help to expand and facilitate their commercial use. With this goal, we evaluated the application of 1-alkyl-3-methylimidazolium chloride-based ([Cnmim]Cl) ionic liquids (ILs) as additives to preserve the activity of Enhanced Green Fluorescent Protein (EGFP) at different storage times and under unfavorable conditions. All [Cnmim]Cl solutions (at 0.100 mol L−1) were able to preserve EGFP fluorescence for longer than the phosphate-saline buffer (PBS) and NaCl solutions, increasing its fluorescence manifestation from 1 to 3 months. [Cnmim]Cl with shorter to medium cationic alkyl chains were the most effective in preserving EGFP fluorescence. [Cnmim]Cl also protected EGFP activity in the presence of the surfactant SDS, the acid guanidine hydrochloride, and H2O2. Therefore, [Cnmim]Cl can be added to aqueous solutions to preserve EGFP fluorescence activity at room temperature for longer storage times and to reduce the negative impact of denaturing agents on EGFP. Therefore, there is a massive potential for the application of ILs as additives to preserve FP in the long-term without refrigeration and under unfavorable conditions, and this is fundamental to enable expansion of FP in industrial and commercial applications.
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spelling Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activityFluorescenceGreen Fluorescent ProteinIonic liquidsPreservativesProtein stabilityFluorescent Proteins (FP) can be applied as biomarkers and biosensors in the industrial and medical fields, but their large-scale use, especially for new industrial applications, is limited due to their low stability. Hence, the discovery of additives capable of preserving the activity of FP at room temperature and under stress conditions can help to expand and facilitate their commercial use. With this goal, we evaluated the application of 1-alkyl-3-methylimidazolium chloride-based ([Cnmim]Cl) ionic liquids (ILs) as additives to preserve the activity of Enhanced Green Fluorescent Protein (EGFP) at different storage times and under unfavorable conditions. All [Cnmim]Cl solutions (at 0.100 mol L−1) were able to preserve EGFP fluorescence for longer than the phosphate-saline buffer (PBS) and NaCl solutions, increasing its fluorescence manifestation from 1 to 3 months. [Cnmim]Cl with shorter to medium cationic alkyl chains were the most effective in preserving EGFP fluorescence. [Cnmim]Cl also protected EGFP activity in the presence of the surfactant SDS, the acid guanidine hydrochloride, and H2O2. Therefore, [Cnmim]Cl can be added to aqueous solutions to preserve EGFP fluorescence activity at room temperature for longer storage times and to reduce the negative impact of denaturing agents on EGFP. Therefore, there is a massive potential for the application of ILs as additives to preserve FP in the long-term without refrigeration and under unfavorable conditions, and this is fundamental to enable expansion of FP in industrial and commercial applications.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)ASCRS Research FoundationCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação para a Ciência e a TecnologiaDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara-Jaú/Km 01School of Science College of Science Engineering and Health RMIT University, 124 La Trobe StreetSchool of Pharmaceutical Sciences São Paulo University (USP), Av. Prof. Lineu Prestes, no. 580, B16, 05508-000, Cidade de UniversitáriaAustralian Synchrotron, 800 Blackburn RoadUniv Coimbra CIEPQPF Department of Chemical Engineering, Rua Sílvio Lima, Pólo II – Pinhal de MarrocosDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara-Jaú/Km 01CAPES: 001FAPESP: 2014/16424–7FAPESP: 2014/19793–3FAPESP: 2016/07529–5FAPESP: 2018/01858–2FAPESP: 2018/06576–5FAPESP: 2018/20833–0FAPESP: 2018/25511–1FAPESP: 2018/50009–8FAPESP: 2020/14144–8Fundação para a Ciência e a Tecnologia: UIDB/EQU/00102/2020Fundação para a Ciência e a Tecnologia: UIDP/EQU/00102/2020Universidade Estadual Paulista (UNESP)RMIT UniversityUniversidade de São Paulo (USP)Australian SynchrotronCIEPQPFVeríssimo, Nathalia V. [UNESP]Saponi, Carolina F. [UNESP]Ryan, Timothy M.Greaves, Tamar L.Pereira, Jorge F.B. [UNESP]2022-04-28T19:50:21Z2022-04-28T19:50:21Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article412-422http://dx.doi.org/10.1016/j.gce.2021.08.001Green Chemical Engineering, v. 2, n. 4, p. 412-422, 2021.2666-95282096-9147http://hdl.handle.net/11449/22339910.1016/j.gce.2021.08.0012-s2.0-85123982467Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGreen Chemical Engineeringinfo:eu-repo/semantics/openAccess2022-04-28T19:50:21Zoai:repositorio.unesp.br:11449/223399Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:25:37.110148Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity
title Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity
spellingShingle Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity
Veríssimo, Nathalia V. [UNESP]
Fluorescence
Green Fluorescent Protein
Ionic liquids
Preservatives
Protein stability
title_short Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity
title_full Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity
title_fullStr Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity
title_full_unstemmed Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity
title_sort Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity
author Veríssimo, Nathalia V. [UNESP]
author_facet Veríssimo, Nathalia V. [UNESP]
Saponi, Carolina F. [UNESP]
Ryan, Timothy M.
Greaves, Tamar L.
Pereira, Jorge F.B. [UNESP]
author_role author
author2 Saponi, Carolina F. [UNESP]
Ryan, Timothy M.
Greaves, Tamar L.
Pereira, Jorge F.B. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
RMIT University
Universidade de São Paulo (USP)
Australian Synchrotron
CIEPQPF
dc.contributor.author.fl_str_mv Veríssimo, Nathalia V. [UNESP]
Saponi, Carolina F. [UNESP]
Ryan, Timothy M.
Greaves, Tamar L.
Pereira, Jorge F.B. [UNESP]
dc.subject.por.fl_str_mv Fluorescence
Green Fluorescent Protein
Ionic liquids
Preservatives
Protein stability
topic Fluorescence
Green Fluorescent Protein
Ionic liquids
Preservatives
Protein stability
description Fluorescent Proteins (FP) can be applied as biomarkers and biosensors in the industrial and medical fields, but their large-scale use, especially for new industrial applications, is limited due to their low stability. Hence, the discovery of additives capable of preserving the activity of FP at room temperature and under stress conditions can help to expand and facilitate their commercial use. With this goal, we evaluated the application of 1-alkyl-3-methylimidazolium chloride-based ([Cnmim]Cl) ionic liquids (ILs) as additives to preserve the activity of Enhanced Green Fluorescent Protein (EGFP) at different storage times and under unfavorable conditions. All [Cnmim]Cl solutions (at 0.100 mol L−1) were able to preserve EGFP fluorescence for longer than the phosphate-saline buffer (PBS) and NaCl solutions, increasing its fluorescence manifestation from 1 to 3 months. [Cnmim]Cl with shorter to medium cationic alkyl chains were the most effective in preserving EGFP fluorescence. [Cnmim]Cl also protected EGFP activity in the presence of the surfactant SDS, the acid guanidine hydrochloride, and H2O2. Therefore, [Cnmim]Cl can be added to aqueous solutions to preserve EGFP fluorescence activity at room temperature for longer storage times and to reduce the negative impact of denaturing agents on EGFP. Therefore, there is a massive potential for the application of ILs as additives to preserve FP in the long-term without refrigeration and under unfavorable conditions, and this is fundamental to enable expansion of FP in industrial and commercial applications.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-01
2022-04-28T19:50:21Z
2022-04-28T19:50:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.gce.2021.08.001
Green Chemical Engineering, v. 2, n. 4, p. 412-422, 2021.
2666-9528
2096-9147
http://hdl.handle.net/11449/223399
10.1016/j.gce.2021.08.001
2-s2.0-85123982467
url http://dx.doi.org/10.1016/j.gce.2021.08.001
http://hdl.handle.net/11449/223399
identifier_str_mv Green Chemical Engineering, v. 2, n. 4, p. 412-422, 2021.
2666-9528
2096-9147
10.1016/j.gce.2021.08.001
2-s2.0-85123982467
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Green Chemical Engineering
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 412-422
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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