Transcriptional profiling of embryo cryotolerance
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/mrd.23436 http://hdl.handle.net/11449/205565 |
Resumo: | The cryosurvival of embryos is a complex process involving dynamic and integrated morphological, functional, and molecular changes. Here, we evaluated the transcriptional profiling of bovine embryos possessing high and low cryotolerance (HC and LC, respectively) by assessing the resumption of development. Embryos were produced in vitro (N = 1137) and cryopreserved (N = 894). Blastocysts samples possessed pronounced group individualization at RNA sequencing. A total of 114 genes were differentially expressed, and 27 and 84 genes were upregulated in HC and LC, respectively. Among the over-represented biological functions, cellular growth and proliferation, cell death and survival, and organismal survival were predicted to be activated, while cellular movement and cell-to-cell signaling were predicted to be inhibited in HC embryos. Enriched canonical pathways and upstream regulators related to cellular proliferation and survival (HC), inflammatory processes, and cell death (LC) were predicted to represent two embryonic molecular profiles present during the resumption of development after cryopreservation. The marked contrast in transcriptional profiles between HC and LC strongly suggests the influence of embryonic competence after cryopreservation on its respective transcriptome and indicated that HC and LC presented two different molecular strategies to overcome cryopreservation-related stress and resume postcryopreservation development. |
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Transcriptional profiling of embryo cryotolerancebovinecryosurvivalembryonic competencepostcryopreservation resumption of developmentqualityThe cryosurvival of embryos is a complex process involving dynamic and integrated morphological, functional, and molecular changes. Here, we evaluated the transcriptional profiling of bovine embryos possessing high and low cryotolerance (HC and LC, respectively) by assessing the resumption of development. Embryos were produced in vitro (N = 1137) and cryopreserved (N = 894). Blastocysts samples possessed pronounced group individualization at RNA sequencing. A total of 114 genes were differentially expressed, and 27 and 84 genes were upregulated in HC and LC, respectively. Among the over-represented biological functions, cellular growth and proliferation, cell death and survival, and organismal survival were predicted to be activated, while cellular movement and cell-to-cell signaling were predicted to be inhibited in HC embryos. Enriched canonical pathways and upstream regulators related to cellular proliferation and survival (HC), inflammatory processes, and cell death (LC) were predicted to represent two embryonic molecular profiles present during the resumption of development after cryopreservation. The marked contrast in transcriptional profiles between HC and LC strongly suggests the influence of embryonic competence after cryopreservation on its respective transcriptome and indicated that HC and LC presented two different molecular strategies to overcome cryopreservation-related stress and resume postcryopreservation development.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Center for Natural and Human Sciences Federal University of ABCSchool of Veterinary Medicine Federal University of PampaIn vitro Brazil – IVB ABS PecplanDepartment of Pharmacology Laboratory of Phytomedicines Pharmacology and Biotechnology Institute of Biosciences University of São Paulo State (Unesp)Department of Genetics and Evolutionary Biology University of São PauloDepartment of Biological Science School of Sciences and Languages University of São Paulo StateDepartment of Genetics and Evolution Federal University of São CarlosDepartment of Pharmacology Laboratory of Phytomedicines Pharmacology and Biotechnology Institute of Biosciences University of São Paulo State (Unesp)Federal University of ABCFederal University of PampaABS PecplanUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Federal de São Carlos (UFSCar)Marsico, Thamiris V.Caetano, Diana P.Rodrigues, RafaelaValente, Roniele S.Fontes, Patricia K. [UNESP]Mesquita, Fernando S.Andrade, Sónia C. da SilvaBasso, Andréa C.Nogueira, Marcelo F. G. [UNESP]Sudano, Mateus J.2021-06-25T10:17:29Z2021-06-25T10:17:29Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1245-1259http://dx.doi.org/10.1002/mrd.23436Molecular Reproduction and Development, v. 87, n. 12, p. 1245-1259, 2020.1098-27951040-452Xhttp://hdl.handle.net/11449/20556510.1002/mrd.234362-s2.0-85097201057Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular Reproduction and Developmentinfo:eu-repo/semantics/openAccess2021-10-23T14:54:04Zoai:repositorio.unesp.br:11449/205565Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T14:54:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transcriptional profiling of embryo cryotolerance |
title |
Transcriptional profiling of embryo cryotolerance |
spellingShingle |
Transcriptional profiling of embryo cryotolerance Marsico, Thamiris V. bovine cryosurvival embryonic competence postcryopreservation resumption of development quality |
title_short |
Transcriptional profiling of embryo cryotolerance |
title_full |
Transcriptional profiling of embryo cryotolerance |
title_fullStr |
Transcriptional profiling of embryo cryotolerance |
title_full_unstemmed |
Transcriptional profiling of embryo cryotolerance |
title_sort |
Transcriptional profiling of embryo cryotolerance |
author |
Marsico, Thamiris V. |
author_facet |
Marsico, Thamiris V. Caetano, Diana P. Rodrigues, Rafaela Valente, Roniele S. Fontes, Patricia K. [UNESP] Mesquita, Fernando S. Andrade, Sónia C. da Silva Basso, Andréa C. Nogueira, Marcelo F. G. [UNESP] Sudano, Mateus J. |
author_role |
author |
author2 |
Caetano, Diana P. Rodrigues, Rafaela Valente, Roniele S. Fontes, Patricia K. [UNESP] Mesquita, Fernando S. Andrade, Sónia C. da Silva Basso, Andréa C. Nogueira, Marcelo F. G. [UNESP] Sudano, Mateus J. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of ABC Federal University of Pampa ABS Pecplan Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Marsico, Thamiris V. Caetano, Diana P. Rodrigues, Rafaela Valente, Roniele S. Fontes, Patricia K. [UNESP] Mesquita, Fernando S. Andrade, Sónia C. da Silva Basso, Andréa C. Nogueira, Marcelo F. G. [UNESP] Sudano, Mateus J. |
dc.subject.por.fl_str_mv |
bovine cryosurvival embryonic competence postcryopreservation resumption of development quality |
topic |
bovine cryosurvival embryonic competence postcryopreservation resumption of development quality |
description |
The cryosurvival of embryos is a complex process involving dynamic and integrated morphological, functional, and molecular changes. Here, we evaluated the transcriptional profiling of bovine embryos possessing high and low cryotolerance (HC and LC, respectively) by assessing the resumption of development. Embryos were produced in vitro (N = 1137) and cryopreserved (N = 894). Blastocysts samples possessed pronounced group individualization at RNA sequencing. A total of 114 genes were differentially expressed, and 27 and 84 genes were upregulated in HC and LC, respectively. Among the over-represented biological functions, cellular growth and proliferation, cell death and survival, and organismal survival were predicted to be activated, while cellular movement and cell-to-cell signaling were predicted to be inhibited in HC embryos. Enriched canonical pathways and upstream regulators related to cellular proliferation and survival (HC), inflammatory processes, and cell death (LC) were predicted to represent two embryonic molecular profiles present during the resumption of development after cryopreservation. The marked contrast in transcriptional profiles between HC and LC strongly suggests the influence of embryonic competence after cryopreservation on its respective transcriptome and indicated that HC and LC presented two different molecular strategies to overcome cryopreservation-related stress and resume postcryopreservation development. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2021-06-25T10:17:29Z 2021-06-25T10:17:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/mrd.23436 Molecular Reproduction and Development, v. 87, n. 12, p. 1245-1259, 2020. 1098-2795 1040-452X http://hdl.handle.net/11449/205565 10.1002/mrd.23436 2-s2.0-85097201057 |
url |
http://dx.doi.org/10.1002/mrd.23436 http://hdl.handle.net/11449/205565 |
identifier_str_mv |
Molecular Reproduction and Development, v. 87, n. 12, p. 1245-1259, 2020. 1098-2795 1040-452X 10.1002/mrd.23436 2-s2.0-85097201057 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular Reproduction and Development |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1245-1259 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964586385866752 |