Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophages

Detalhes bibliográficos
Autor(a) principal: Bazon, Murilo Luiz [UNESP]
Data de Publicação: 2021
Outros Autores: Fernandes, Luis Gustavo Romani, Assugeni, Isabela Oliveira Sandrini [UNESP], Pinto, Lucas Machado [UNESP], Simioni, Patrícia Ucelli, de Lima Zollner, Ricardo, Braga, Márcia Regina Brochetto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/toxins13120850
http://hdl.handle.net/11449/230018
Resumo: The social wasp Polybia paulista (Hymenoptera, Vespidae) is highly aggressive, being responsible for many medical occurrences. One of the most allergenic components of this venom is Antigen 5 (Poly p 5). The possible modulation of the in vitro immune response induced by antigen 5 from P. paulista venom, expressed recombinantly (rPoly p 5), on BALB/c mice peritoneal macrophages, activated or not with LPS, was assessed. Here, we analyzed cell viability changes, expression of the phosphorylated form of p65 NF-κB subunit, nitric oxide (NO), proinflammatory cytokines production, and co-stimulatory molecules (CD80, CD86). The results suggest that rPoly p 5 does not affect NO production nor the expression of co-stimulatory molecules in mouse peritoneal macrophages. On the other hand, rPoly p 5 induced an increase in IL-1β production in non-activated macrophages and a reduction in the production of TNF-α and MCP-1 cytokines in activated macrophages. rPoly p 5 decreased the in vitro production of the phosphorylated p65 NF-κB subunit in non-activated macrophages. These findings suggest an essential role of this allergen in the polarization of functional M2 macrophage phenotypes, when analyzed in previously activated macrophages. Further investigations, mainly in in vivo studies, should be conducted to elucidate Polybia paulista Ag5 biological role in the macrophage functional profile modulation.
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spelling Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophagesAntigen 5HymenopteraInflammatory effectsMacrophagesPolybia paulistaThe social wasp Polybia paulista (Hymenoptera, Vespidae) is highly aggressive, being responsible for many medical occurrences. One of the most allergenic components of this venom is Antigen 5 (Poly p 5). The possible modulation of the in vitro immune response induced by antigen 5 from P. paulista venom, expressed recombinantly (rPoly p 5), on BALB/c mice peritoneal macrophages, activated or not with LPS, was assessed. Here, we analyzed cell viability changes, expression of the phosphorylated form of p65 NF-κB subunit, nitric oxide (NO), proinflammatory cytokines production, and co-stimulatory molecules (CD80, CD86). The results suggest that rPoly p 5 does not affect NO production nor the expression of co-stimulatory molecules in mouse peritoneal macrophages. On the other hand, rPoly p 5 induced an increase in IL-1β production in non-activated macrophages and a reduction in the production of TNF-α and MCP-1 cytokines in activated macrophages. rPoly p 5 decreased the in vitro production of the phosphorylated p65 NF-κB subunit in non-activated macrophages. These findings suggest an essential role of this allergen in the polarization of functional M2 macrophage phenotypes, when analyzed in previously activated macrophages. Further investigations, mainly in in vivo studies, should be conducted to elucidate Polybia paulista Ag5 biological role in the macrophage functional profile modulation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Biologia Molecular de Artrópodes—LBMA Instituto de Biologia—IB Universidade Estadual Paulista Câmpus Rio Claro—UNESP-RC, Avenida 24-A, n◦1515, Bela VistaLaboratório de Imunologia Translacional—LIT Departamento de Clínica Médica Faculdade de Ciências Médicas—FCM Universidade Estadual de Campinas—UNICAMP Cidade Universitária “Zeferino Vaz”, Rua Tessália Vieira de Camargo, n◦126Departamento de Biomedicina Faculdade de Americana—FAM, Avenida Joaquim Bôer, n◦733, Jardim LucianeFaculdade de Medicina Universidade Anhembi Morumbi, Av. Rio das Pedras, 1601-PompéiaLaboratório de Biologia Molecular de Artrópodes—LBMA Instituto de Biologia—IB Universidade Estadual Paulista Câmpus Rio Claro—UNESP-RC, Avenida 24-A, n◦1515, Bela VistaFAPESP: 2017/07988-2FAPESP: 2017/18422-0FAPESP: 2019/02298-3Universidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Faculdade de Americana—FAMUniversidade Anhembi MorumbiBazon, Murilo Luiz [UNESP]Fernandes, Luis Gustavo RomaniAssugeni, Isabela Oliveira Sandrini [UNESP]Pinto, Lucas Machado [UNESP]Simioni, Patrícia Ucellide Lima Zollner, RicardoBraga, Márcia Regina Brochetto [UNESP]2022-04-29T08:37:13Z2022-04-29T08:37:13Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/toxins13120850Toxins, v. 13, n. 12, 2021.2072-6651http://hdl.handle.net/11449/23001810.3390/toxins131208502-s2.0-85120798406Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxinsinfo:eu-repo/semantics/openAccess2022-04-29T08:37:13Zoai:repositorio.unesp.br:11449/230018Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:41:40.604415Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophages
title Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophages
spellingShingle Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophages
Bazon, Murilo Luiz [UNESP]
Antigen 5
Hymenoptera
Inflammatory effects
Macrophages
Polybia paulista
title_short Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophages
title_full Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophages
title_fullStr Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophages
title_full_unstemmed Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophages
title_sort Biological and inflammatory effects of antigen 5 from polybia paulista (Hymenoptera, vespidae) venom in mouse intraperitoneal macrophages
author Bazon, Murilo Luiz [UNESP]
author_facet Bazon, Murilo Luiz [UNESP]
Fernandes, Luis Gustavo Romani
Assugeni, Isabela Oliveira Sandrini [UNESP]
Pinto, Lucas Machado [UNESP]
Simioni, Patrícia Ucelli
de Lima Zollner, Ricardo
Braga, Márcia Regina Brochetto [UNESP]
author_role author
author2 Fernandes, Luis Gustavo Romani
Assugeni, Isabela Oliveira Sandrini [UNESP]
Pinto, Lucas Machado [UNESP]
Simioni, Patrícia Ucelli
de Lima Zollner, Ricardo
Braga, Márcia Regina Brochetto [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Estadual de Campinas (UNICAMP)
Faculdade de Americana—FAM
Universidade Anhembi Morumbi
dc.contributor.author.fl_str_mv Bazon, Murilo Luiz [UNESP]
Fernandes, Luis Gustavo Romani
Assugeni, Isabela Oliveira Sandrini [UNESP]
Pinto, Lucas Machado [UNESP]
Simioni, Patrícia Ucelli
de Lima Zollner, Ricardo
Braga, Márcia Regina Brochetto [UNESP]
dc.subject.por.fl_str_mv Antigen 5
Hymenoptera
Inflammatory effects
Macrophages
Polybia paulista
topic Antigen 5
Hymenoptera
Inflammatory effects
Macrophages
Polybia paulista
description The social wasp Polybia paulista (Hymenoptera, Vespidae) is highly aggressive, being responsible for many medical occurrences. One of the most allergenic components of this venom is Antigen 5 (Poly p 5). The possible modulation of the in vitro immune response induced by antigen 5 from P. paulista venom, expressed recombinantly (rPoly p 5), on BALB/c mice peritoneal macrophages, activated or not with LPS, was assessed. Here, we analyzed cell viability changes, expression of the phosphorylated form of p65 NF-κB subunit, nitric oxide (NO), proinflammatory cytokines production, and co-stimulatory molecules (CD80, CD86). The results suggest that rPoly p 5 does not affect NO production nor the expression of co-stimulatory molecules in mouse peritoneal macrophages. On the other hand, rPoly p 5 induced an increase in IL-1β production in non-activated macrophages and a reduction in the production of TNF-α and MCP-1 cytokines in activated macrophages. rPoly p 5 decreased the in vitro production of the phosphorylated p65 NF-κB subunit in non-activated macrophages. These findings suggest an essential role of this allergen in the polarization of functional M2 macrophage phenotypes, when analyzed in previously activated macrophages. Further investigations, mainly in in vivo studies, should be conducted to elucidate Polybia paulista Ag5 biological role in the macrophage functional profile modulation.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-01
2022-04-29T08:37:13Z
2022-04-29T08:37:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/toxins13120850
Toxins, v. 13, n. 12, 2021.
2072-6651
http://hdl.handle.net/11449/230018
10.3390/toxins13120850
2-s2.0-85120798406
url http://dx.doi.org/10.3390/toxins13120850
http://hdl.handle.net/11449/230018
identifier_str_mv Toxins, v. 13, n. 12, 2021.
2072-6651
10.3390/toxins13120850
2-s2.0-85120798406
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxins
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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