Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidase
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/10826068.2019.1625060 http://hdl.handle.net/11449/185891 |
Resumo: | The current study evaluated the production and characterization of beta-glucosidase by the thermophilic fungus Thermomucor indicae-seudaticae in solid-state fermentation of wheat bran. Isolated fungi have significant amounts of beta-glucosidase, an enzyme that may be applied to different industrial processes, such as the production of fuels, food, and other chemical compounds. Maximal enzyme activity occurred in pH 3.5-4.5 and at 70 degrees C. The enzyme exhibited high thermostability, for 1 h, up to 60 degrees C, and good tolerance to glucose (10 mM) and ethanol (10%). The optimization of fermentative parameters on the production of beta-glucosidase was carried out by evaluating the best supplementary nutrient source, pH of nutrient solution, initial substrate moisture and fermentation temperature. The optimization of the above fermentation parameters increased enzyme activity by 120.0%. The highest enzymatic activity (164.0 U/g) occurred with wheat bran containing 70% initial moisture, supplemented with 1.0% (NH4)(2)SO4 solution at pH 5.5-6.0 and fungus incubated at 40 degrees C. A more detailed study of beta-glucosidase suggested that Sulfur is an important component of the main amino acid present in this enzyme. The enhancer of the enzyme activity occurred when the fungus was grown on wheat bran supplemented with a sulfur-containing solution. In fact, increasing the concentration of sulfur in the solution increased its activity. |
id |
UNSP_70046f2d52c0140a999d077d0d2b5f49 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/185891 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidasebeta-glucosidasesolid-state fermentationsulfurwheat branThe current study evaluated the production and characterization of beta-glucosidase by the thermophilic fungus Thermomucor indicae-seudaticae in solid-state fermentation of wheat bran. Isolated fungi have significant amounts of beta-glucosidase, an enzyme that may be applied to different industrial processes, such as the production of fuels, food, and other chemical compounds. Maximal enzyme activity occurred in pH 3.5-4.5 and at 70 degrees C. The enzyme exhibited high thermostability, for 1 h, up to 60 degrees C, and good tolerance to glucose (10 mM) and ethanol (10%). The optimization of fermentative parameters on the production of beta-glucosidase was carried out by evaluating the best supplementary nutrient source, pH of nutrient solution, initial substrate moisture and fermentation temperature. The optimization of the above fermentation parameters increased enzyme activity by 120.0%. The highest enzymatic activity (164.0 U/g) occurred with wheat bran containing 70% initial moisture, supplemented with 1.0% (NH4)(2)SO4 solution at pH 5.5-6.0 and fungus incubated at 40 degrees C. A more detailed study of beta-glucosidase suggested that Sulfur is an important component of the main amino acid present in this enzyme. The enhancer of the enzyme activity occurred when the fungus was grown on wheat bran supplemented with a sulfur-containing solution. In fact, increasing the concentration of sulfur in the solution increased its activity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade do Estado de Minas Gerais (UEMG)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Univ Estado Minas Gerais, Lab Microbiol, Campus Frutal,Ave Prof Mario Palmerio 1001, BR-38200000 Frutal, BrazilUniv Estadual Paulista, Lab Bioquim & Microbiol Aplicada, Sao Jose Do Rio Preto, BrazilUniv Estadual Paulista, Lab Ecol & Sistemat Fungos, Rio Claro, BrazilUniv Estadual Paulista, Lab Bioquim & Microbiol Aplicada, Sao Jose Do Rio Preto, BrazilUniv Estadual Paulista, Lab Ecol & Sistemat Fungos, Rio Claro, BrazilTaylor & Francis IncUniv Estado Minas GeraisUniversidade Estadual Paulista (Unesp)Martins, Eduardo da SilvaGomes, Eleni [UNESP]Silva, Roberto da [UNESP]Bizarria Junior, Rodolfo [UNESP]2019-10-04T12:39:33Z2019-10-04T12:39:33Z2019-06-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article830-836http://dx.doi.org/10.1080/10826068.2019.1625060Preparative Biochemistry & Biotechnology. Philadelphia: Taylor & Francis Inc, v. 49, n. 8, p. 830-836, 2019.1082-6068http://hdl.handle.net/11449/18589110.1080/10826068.2019.1625060WOS:000475245400001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPreparative Biochemistry & Biotechnologyinfo:eu-repo/semantics/openAccess2021-10-23T04:16:10Zoai:repositorio.unesp.br:11449/185891Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:28:49.621771Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidase |
title |
Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidase |
spellingShingle |
Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidase Martins, Eduardo da Silva beta-glucosidase solid-state fermentation sulfur wheat bran |
title_short |
Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidase |
title_full |
Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidase |
title_fullStr |
Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidase |
title_full_unstemmed |
Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidase |
title_sort |
Production of cellulases by Thermomucor indicae-seudaticae: characterization of a thermophilic beta-glucosidase |
author |
Martins, Eduardo da Silva |
author_facet |
Martins, Eduardo da Silva Gomes, Eleni [UNESP] Silva, Roberto da [UNESP] Bizarria Junior, Rodolfo [UNESP] |
author_role |
author |
author2 |
Gomes, Eleni [UNESP] Silva, Roberto da [UNESP] Bizarria Junior, Rodolfo [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Univ Estado Minas Gerais Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Martins, Eduardo da Silva Gomes, Eleni [UNESP] Silva, Roberto da [UNESP] Bizarria Junior, Rodolfo [UNESP] |
dc.subject.por.fl_str_mv |
beta-glucosidase solid-state fermentation sulfur wheat bran |
topic |
beta-glucosidase solid-state fermentation sulfur wheat bran |
description |
The current study evaluated the production and characterization of beta-glucosidase by the thermophilic fungus Thermomucor indicae-seudaticae in solid-state fermentation of wheat bran. Isolated fungi have significant amounts of beta-glucosidase, an enzyme that may be applied to different industrial processes, such as the production of fuels, food, and other chemical compounds. Maximal enzyme activity occurred in pH 3.5-4.5 and at 70 degrees C. The enzyme exhibited high thermostability, for 1 h, up to 60 degrees C, and good tolerance to glucose (10 mM) and ethanol (10%). The optimization of fermentative parameters on the production of beta-glucosidase was carried out by evaluating the best supplementary nutrient source, pH of nutrient solution, initial substrate moisture and fermentation temperature. The optimization of the above fermentation parameters increased enzyme activity by 120.0%. The highest enzymatic activity (164.0 U/g) occurred with wheat bran containing 70% initial moisture, supplemented with 1.0% (NH4)(2)SO4 solution at pH 5.5-6.0 and fungus incubated at 40 degrees C. A more detailed study of beta-glucosidase suggested that Sulfur is an important component of the main amino acid present in this enzyme. The enhancer of the enzyme activity occurred when the fungus was grown on wheat bran supplemented with a sulfur-containing solution. In fact, increasing the concentration of sulfur in the solution increased its activity. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:39:33Z 2019-10-04T12:39:33Z 2019-06-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/10826068.2019.1625060 Preparative Biochemistry & Biotechnology. Philadelphia: Taylor & Francis Inc, v. 49, n. 8, p. 830-836, 2019. 1082-6068 http://hdl.handle.net/11449/185891 10.1080/10826068.2019.1625060 WOS:000475245400001 |
url |
http://dx.doi.org/10.1080/10826068.2019.1625060 http://hdl.handle.net/11449/185891 |
identifier_str_mv |
Preparative Biochemistry & Biotechnology. Philadelphia: Taylor & Francis Inc, v. 49, n. 8, p. 830-836, 2019. 1082-6068 10.1080/10826068.2019.1625060 WOS:000475245400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Preparative Biochemistry & Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
830-836 |
dc.publisher.none.fl_str_mv |
Taylor & Francis Inc |
publisher.none.fl_str_mv |
Taylor & Francis Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128519025197056 |