Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juveniles
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.envpol.2019.05.076 http://hdl.handle.net/11449/187679 |
Resumo: | Genotoxic analyses are commonly used in ecotoxicological studies as early biomarkers to investigate the potential effects of environmental contaminants on biological models. Several pollutants can induce DNA damage and, therefore, counting micronuclei and other nuclear abnormalities are efficient tools to evaluate genotoxicity. Some pollutants such as 4-nonylphenol (NP), a detergent used mainly in industries, and Cyproterone Acetate (CPA), an antiandrogenic medicine, have already shown genotoxic effects on some vertebrates. However, although amphibians are considered bioindicators of environmental quality and their populations are declining worldwide, the effects of these compounds on anurans are not yet known and, therefore, we believe that it is important to investigate such effects on anurans. Since water contamination is one of the ultimate causes of amphibian decline, ecotoxicological studies are important to discuss the appropriate solutions to avoid species extinction. Thus, this study investigates the genotoxic effects on Rana catesbeiana tadpoles and juveniles after being exposed to 1, 10 and 100 μg/L NP and 0.025, 0.25 and 2.5 ng/L CPA, by counting the nuclear abnormalities after exposure. The laboratory experiments lasted 28 days. The experimental conditions were the same except for the water volume since tadpoles and juveniles exhibit different habits at different developmental stages. Compared to juveniles, tadpoles were more susceptible to both compounds as indicated by the increased nuclear abnormalities observed in the highest NP concentration and all tested CPA concentrations. The juveniles, on the other hand, responded only to the two highest CPA concentrations. We concluded that CPA, even at very low concentrations, is extremely harmful to both anuran developmental stages and, particularly, to tadpoles. The significant effects observed on tadpoles is an important outcome of this study since 100 μg/L or higher NP concentrations are frequently detected in the environment. |
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Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juvenilesBioindicatorBiomarkersEDCsMicronucleiNuclear abnormalitiesGenotoxic analyses are commonly used in ecotoxicological studies as early biomarkers to investigate the potential effects of environmental contaminants on biological models. Several pollutants can induce DNA damage and, therefore, counting micronuclei and other nuclear abnormalities are efficient tools to evaluate genotoxicity. Some pollutants such as 4-nonylphenol (NP), a detergent used mainly in industries, and Cyproterone Acetate (CPA), an antiandrogenic medicine, have already shown genotoxic effects on some vertebrates. However, although amphibians are considered bioindicators of environmental quality and their populations are declining worldwide, the effects of these compounds on anurans are not yet known and, therefore, we believe that it is important to investigate such effects on anurans. Since water contamination is one of the ultimate causes of amphibian decline, ecotoxicological studies are important to discuss the appropriate solutions to avoid species extinction. Thus, this study investigates the genotoxic effects on Rana catesbeiana tadpoles and juveniles after being exposed to 1, 10 and 100 μg/L NP and 0.025, 0.25 and 2.5 ng/L CPA, by counting the nuclear abnormalities after exposure. The laboratory experiments lasted 28 days. The experimental conditions were the same except for the water volume since tadpoles and juveniles exhibit different habits at different developmental stages. Compared to juveniles, tadpoles were more susceptible to both compounds as indicated by the increased nuclear abnormalities observed in the highest NP concentration and all tested CPA concentrations. The juveniles, on the other hand, responded only to the two highest CPA concentrations. We concluded that CPA, even at very low concentrations, is extremely harmful to both anuran developmental stages and, particularly, to tadpoles. The significant effects observed on tadpoles is an important outcome of this study since 100 μg/L or higher NP concentrations are frequently detected in the environment.This study emphasizes the importance of knowing how anurans in different developmental stages respond to the genotoxic effects of pollutants such as NP and CPA when exposed to environmentally relevant doses.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Graduate Program in Animal Biology Department of Biology São Paulo State University (UNESP)Department of Biology São Paulo State University (UNESP)Laboratory of Experimental Pathology (LAPEx) Federal University of Mato Grosso Do Sul (UFMS) Institute of Biosciences (INBIO)Graduate Program in Animal Biology Department of Biology São Paulo State University (UNESP)Department of Biology São Paulo State University (UNESP)FAPESP: 2013/02067-5FAPESP: 2014/00946-4FAPESP: 2018/01078-7CNPq: 305081/2015-2Universidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Gregorio, L. S. [UNESP]Franco-Belussi, L. [UNESP]De Oliveira, C. [UNESP]2019-10-06T15:43:57Z2019-10-06T15:43:57Z2019-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article879-884http://dx.doi.org/10.1016/j.envpol.2019.05.076Environmental Pollution, v. 251, p. 879-884.1873-64240269-7491http://hdl.handle.net/11449/18767910.1016/j.envpol.2019.05.0762-s2.0-85065881800Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Pollutioninfo:eu-repo/semantics/openAccess2021-10-23T19:49:55Zoai:repositorio.unesp.br:11449/187679Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:00:16.765265Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juveniles |
title |
Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juveniles |
spellingShingle |
Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juveniles Gregorio, L. S. [UNESP] Bioindicator Biomarkers EDCs Micronuclei Nuclear abnormalities |
title_short |
Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juveniles |
title_full |
Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juveniles |
title_fullStr |
Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juveniles |
title_full_unstemmed |
Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juveniles |
title_sort |
Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura)tadpoles and juveniles |
author |
Gregorio, L. S. [UNESP] |
author_facet |
Gregorio, L. S. [UNESP] Franco-Belussi, L. [UNESP] De Oliveira, C. [UNESP] |
author_role |
author |
author2 |
Franco-Belussi, L. [UNESP] De Oliveira, C. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) |
dc.contributor.author.fl_str_mv |
Gregorio, L. S. [UNESP] Franco-Belussi, L. [UNESP] De Oliveira, C. [UNESP] |
dc.subject.por.fl_str_mv |
Bioindicator Biomarkers EDCs Micronuclei Nuclear abnormalities |
topic |
Bioindicator Biomarkers EDCs Micronuclei Nuclear abnormalities |
description |
Genotoxic analyses are commonly used in ecotoxicological studies as early biomarkers to investigate the potential effects of environmental contaminants on biological models. Several pollutants can induce DNA damage and, therefore, counting micronuclei and other nuclear abnormalities are efficient tools to evaluate genotoxicity. Some pollutants such as 4-nonylphenol (NP), a detergent used mainly in industries, and Cyproterone Acetate (CPA), an antiandrogenic medicine, have already shown genotoxic effects on some vertebrates. However, although amphibians are considered bioindicators of environmental quality and their populations are declining worldwide, the effects of these compounds on anurans are not yet known and, therefore, we believe that it is important to investigate such effects on anurans. Since water contamination is one of the ultimate causes of amphibian decline, ecotoxicological studies are important to discuss the appropriate solutions to avoid species extinction. Thus, this study investigates the genotoxic effects on Rana catesbeiana tadpoles and juveniles after being exposed to 1, 10 and 100 μg/L NP and 0.025, 0.25 and 2.5 ng/L CPA, by counting the nuclear abnormalities after exposure. The laboratory experiments lasted 28 days. The experimental conditions were the same except for the water volume since tadpoles and juveniles exhibit different habits at different developmental stages. Compared to juveniles, tadpoles were more susceptible to both compounds as indicated by the increased nuclear abnormalities observed in the highest NP concentration and all tested CPA concentrations. The juveniles, on the other hand, responded only to the two highest CPA concentrations. We concluded that CPA, even at very low concentrations, is extremely harmful to both anuran developmental stages and, particularly, to tadpoles. The significant effects observed on tadpoles is an important outcome of this study since 100 μg/L or higher NP concentrations are frequently detected in the environment. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:43:57Z 2019-10-06T15:43:57Z 2019-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.envpol.2019.05.076 Environmental Pollution, v. 251, p. 879-884. 1873-6424 0269-7491 http://hdl.handle.net/11449/187679 10.1016/j.envpol.2019.05.076 2-s2.0-85065881800 |
url |
http://dx.doi.org/10.1016/j.envpol.2019.05.076 http://hdl.handle.net/11449/187679 |
identifier_str_mv |
Environmental Pollution, v. 251, p. 879-884. 1873-6424 0269-7491 10.1016/j.envpol.2019.05.076 2-s2.0-85065881800 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Pollution |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
879-884 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128883443105792 |