Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0967199416000101 http://hdl.handle.net/11449/162160 |
Resumo: | The aim of this study was to describe the morphology of gametes, post-fertilization events and subsequent temperature effects on the early developmental stages of the neotropical species Astyanax altiparanae. The sperm of this species presents a typical morphology of teleost sperm with a spherical head (diameter = 1.88 mu m), midpiece (diameter = 0.75 mu m) and a single flagellum (length = 18.67 mu m). The extrusion of the second polar body and fusion of male and female pronucleus were reported for the first time in this species. Additionally, we observed the formation of the fertilization cone, which prevents polyspermic fertilization. Developmental stages at 22 degrees C, 26 degrees C and 30 degrees C gave rise to fertilization rates at 91.12, 91.42 and 93.04% respectively. Hatching occurred at 25 hpf at 22 degrees C, 16 hpf at 26 degrees C and 11 hpf at 30 degrees C and the hatching rates were 61.78%, 62.90% and 59.45%, respectively. At 22 degrees C, the second polar body was extruded at approximate to 6 mpf and the male and female pronucleus fused at approximate to 10 mpf. This fundamental information is important for the field and opens up new possibilities in fish biotechnology, including micromanipulation and chromosome-set manipulation. |
id |
UNSP_707fe985407ea6815a7f410855cd1f5c |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/162160 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae)EmbryoFishOocyteReproductionSpermatozoaThe aim of this study was to describe the morphology of gametes, post-fertilization events and subsequent temperature effects on the early developmental stages of the neotropical species Astyanax altiparanae. The sperm of this species presents a typical morphology of teleost sperm with a spherical head (diameter = 1.88 mu m), midpiece (diameter = 0.75 mu m) and a single flagellum (length = 18.67 mu m). The extrusion of the second polar body and fusion of male and female pronucleus were reported for the first time in this species. Additionally, we observed the formation of the fertilization cone, which prevents polyspermic fertilization. Developmental stages at 22 degrees C, 26 degrees C and 30 degrees C gave rise to fertilization rates at 91.12, 91.42 and 93.04% respectively. Hatching occurred at 25 hpf at 22 degrees C, 16 hpf at 26 degrees C and 11 hpf at 30 degrees C and the hatching rates were 61.78%, 62.90% and 59.45%, respectively. At 22 degrees C, the second polar body was extruded at approximate to 6 mpf and the male and female pronucleus fused at approximate to 10 mpf. This fundamental information is important for the field and opens up new possibilities in fish biotechnology, including micromanipulation and chromosome-set manipulation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilChico Mendes Inst Biodivers Conservat, Natl Ctr Res & Conservat Continental Fish, Pirassununga, SP, BrazilUniv Sao Paulo, Dept Vet Med FZEA, Ave Duque Caxias Norte 225, BR-13630080 Pirassununga, SP, BrazilHokkaido Univ, Fac Fisheries Sci, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611, JapanChico Mendes Inst Biodivers Conservat, Natl Ctr Res & Conservat Continental, Lab Fish Biotechnol, Rodovia Pref Euberto Nemesio Pereira de Godoy, BR-13630970 Pirassununga, SP, BrazilSao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilCNPq: 131352/2012-1CNPq: 471140/2012-0FAPESP: 2012/18961-4FAPESP: 2010/17429-1Cambridge Univ PressUniversidade Estadual Paulista (Unesp)Chico Mendes Inst Biodivers ConservatUniversidade de São Paulo (USP)Hokkaido UnivSantos, Matheus Pereira dos [UNESP]Yasui, George ShiguekiPorfirio Xavier, Pedro LuizMacedo Adamov, Nadya Soares deNascimento, Nivaldo Ferreira do [UNESP]Fujimoto, TakafumiSenhorini, Jose AugustoOkada Nakaghi, Laura Satiko [UNESP]2018-11-26T17:10:38Z2018-11-26T17:10:38Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article795-807application/pdfhttp://dx.doi.org/10.1017/S0967199416000101Zygote. New York: Cambridge Univ Press, v. 24, n. 6, p. 795-807, 2016.0967-1994http://hdl.handle.net/11449/16216010.1017/S0967199416000101WOS:000387773400001WOS000387773400001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygote0,387info:eu-repo/semantics/openAccess2024-04-09T15:29:46Zoai:repositorio.unesp.br:11449/162160Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:04:47.590364Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae) |
title |
Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae) |
spellingShingle |
Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae) Santos, Matheus Pereira dos [UNESP] Embryo Fish Oocyte Reproduction Spermatozoa |
title_short |
Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae) |
title_full |
Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae) |
title_fullStr |
Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae) |
title_full_unstemmed |
Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae) |
title_sort |
Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae) |
author |
Santos, Matheus Pereira dos [UNESP] |
author_facet |
Santos, Matheus Pereira dos [UNESP] Yasui, George Shigueki Porfirio Xavier, Pedro Luiz Macedo Adamov, Nadya Soares de Nascimento, Nivaldo Ferreira do [UNESP] Fujimoto, Takafumi Senhorini, Jose Augusto Okada Nakaghi, Laura Satiko [UNESP] |
author_role |
author |
author2 |
Yasui, George Shigueki Porfirio Xavier, Pedro Luiz Macedo Adamov, Nadya Soares de Nascimento, Nivaldo Ferreira do [UNESP] Fujimoto, Takafumi Senhorini, Jose Augusto Okada Nakaghi, Laura Satiko [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Chico Mendes Inst Biodivers Conservat Universidade de São Paulo (USP) Hokkaido Univ |
dc.contributor.author.fl_str_mv |
Santos, Matheus Pereira dos [UNESP] Yasui, George Shigueki Porfirio Xavier, Pedro Luiz Macedo Adamov, Nadya Soares de Nascimento, Nivaldo Ferreira do [UNESP] Fujimoto, Takafumi Senhorini, Jose Augusto Okada Nakaghi, Laura Satiko [UNESP] |
dc.subject.por.fl_str_mv |
Embryo Fish Oocyte Reproduction Spermatozoa |
topic |
Embryo Fish Oocyte Reproduction Spermatozoa |
description |
The aim of this study was to describe the morphology of gametes, post-fertilization events and subsequent temperature effects on the early developmental stages of the neotropical species Astyanax altiparanae. The sperm of this species presents a typical morphology of teleost sperm with a spherical head (diameter = 1.88 mu m), midpiece (diameter = 0.75 mu m) and a single flagellum (length = 18.67 mu m). The extrusion of the second polar body and fusion of male and female pronucleus were reported for the first time in this species. Additionally, we observed the formation of the fertilization cone, which prevents polyspermic fertilization. Developmental stages at 22 degrees C, 26 degrees C and 30 degrees C gave rise to fertilization rates at 91.12, 91.42 and 93.04% respectively. Hatching occurred at 25 hpf at 22 degrees C, 16 hpf at 26 degrees C and 11 hpf at 30 degrees C and the hatching rates were 61.78%, 62.90% and 59.45%, respectively. At 22 degrees C, the second polar body was extruded at approximate to 6 mpf and the male and female pronucleus fused at approximate to 10 mpf. This fundamental information is important for the field and opens up new possibilities in fish biotechnology, including micromanipulation and chromosome-set manipulation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 2018-11-26T17:10:38Z 2018-11-26T17:10:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0967199416000101 Zygote. New York: Cambridge Univ Press, v. 24, n. 6, p. 795-807, 2016. 0967-1994 http://hdl.handle.net/11449/162160 10.1017/S0967199416000101 WOS:000387773400001 WOS000387773400001.pdf |
url |
http://dx.doi.org/10.1017/S0967199416000101 http://hdl.handle.net/11449/162160 |
identifier_str_mv |
Zygote. New York: Cambridge Univ Press, v. 24, n. 6, p. 795-807, 2016. 0967-1994 10.1017/S0967199416000101 WOS:000387773400001 WOS000387773400001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zygote 0,387 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
795-807 application/pdf |
dc.publisher.none.fl_str_mv |
Cambridge Univ Press |
publisher.none.fl_str_mv |
Cambridge Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128752899588096 |