Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2017.03.001 http://hdl.handle.net/11449/174300 |
Resumo: | The effect of varying intervals between successive gonadotropin injections on the superovulatory outcomes in anestrous Rideau Arcott ewes superstimulated for ovarian follicular development with multiple doses of porcine FSH (pFSH) was evaluated in a single study. Twenty-five animals received six (1 × 2.5 ml and 5 × 1.25 ml) injections of Folltropin®-V given at 0800 and 1600 h or at 0800 and 2000 h in Group 1 (n = 9) or Group 2 (n = 16), respectively. An i.m. injection of 500 IU of equine chorionic gonadotropin (eCG; Folligon®) was given concurrently with the first pFSH dose. Time of estrus was synchronized among ewes with intravaginal sponges containing 60 mg of medroxyprogesterone acetate (Veramix®) that were left in place for 14 days; sponges were removed at the time of the 5th pFSH injection. Six days after insertion of MAP sponges, all ewes received an i.m. injection of estradiol-17β dissolved in 1 ml of sesame oil (350 μg/ewe) to synchronize follicular wave emergence. Following the last pFSH dose, all animals were given a single i.m. injection of 50 μg of gonadotropin-releasing hormone (GnRH; Cystorelin®) to induce ovulations before placing in a pen with four fertile rams for 36 h. The ovarian responses were assessed and embryos recovered surgically 7 days after GnRH injections. The mean number of corpora lutea was greater (P < 0.05) in Group 1 compared with Group 2 ewes (21.0 ± 2.9 compared with 10.4 ± 1.6, respectively; mean ± SEM) but there was no difference (P > 0.05) in the number of transferable embryos (5.4 ± 2.4 compared with 5.4 ± 1.3/ewe, respectively), and Group 1 animals had significantly more degenerated embryos than Group 2 ewes (2.6 ± 1.2 compared with 0.6 ± 0.3/ewe, respectively). A superovulatory protocol wherein pFSH injections were given at 0800 and 1600 h was more effective in terms of inducing multiple ovulations than the protocol with 12-h intervals between consecutive pFSH doses, but it was not associated with an increased production of transferable quality embryos by anestrous ewes. |
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Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewesAnestrusEstradiolFollicle-stimulating hormoneSheepSuperovulationThe effect of varying intervals between successive gonadotropin injections on the superovulatory outcomes in anestrous Rideau Arcott ewes superstimulated for ovarian follicular development with multiple doses of porcine FSH (pFSH) was evaluated in a single study. Twenty-five animals received six (1 × 2.5 ml and 5 × 1.25 ml) injections of Folltropin®-V given at 0800 and 1600 h or at 0800 and 2000 h in Group 1 (n = 9) or Group 2 (n = 16), respectively. An i.m. injection of 500 IU of equine chorionic gonadotropin (eCG; Folligon®) was given concurrently with the first pFSH dose. Time of estrus was synchronized among ewes with intravaginal sponges containing 60 mg of medroxyprogesterone acetate (Veramix®) that were left in place for 14 days; sponges were removed at the time of the 5th pFSH injection. Six days after insertion of MAP sponges, all ewes received an i.m. injection of estradiol-17β dissolved in 1 ml of sesame oil (350 μg/ewe) to synchronize follicular wave emergence. Following the last pFSH dose, all animals were given a single i.m. injection of 50 μg of gonadotropin-releasing hormone (GnRH; Cystorelin®) to induce ovulations before placing in a pen with four fertile rams for 36 h. The ovarian responses were assessed and embryos recovered surgically 7 days after GnRH injections. The mean number of corpora lutea was greater (P < 0.05) in Group 1 compared with Group 2 ewes (21.0 ± 2.9 compared with 10.4 ± 1.6, respectively; mean ± SEM) but there was no difference (P > 0.05) in the number of transferable embryos (5.4 ± 2.4 compared with 5.4 ± 1.3/ewe, respectively), and Group 1 animals had significantly more degenerated embryos than Group 2 ewes (2.6 ± 1.2 compared with 0.6 ± 0.3/ewe, respectively). A superovulatory protocol wherein pFSH injections were given at 0800 and 1600 h was more effective in terms of inducing multiple ovulations than the protocol with 12-h intervals between consecutive pFSH doses, but it was not associated with an increased production of transferable quality embryos by anestrous ewes.Department of Biomedical Sciences Ontario Veterinary College University of GuelphDepartment of Animal Biotechnology Agricultural University of KrakówDepartment of Swine and Small Animal Breeding Agricultural University of KrakówDepartment of Preventative Veterinary Medicine and Animal Reproduction School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), JaboticabalDepartment of Preventative Veterinary Medicine and Animal Reproduction School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), JaboticabalUniversity of GuelphAgricultural University of KrakówUniversidade Estadual Paulista (Unesp)Bartlewski, P. M.Murawski, M.Schwarz, T.Oliveira, M. E.F. [UNESP]2018-12-11T17:10:20Z2018-12-11T17:10:20Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article44-49application/pdfhttp://dx.doi.org/10.1016/j.anireprosci.2017.03.001Animal Reproduction Science, v. 180, p. 44-49.0378-4320http://hdl.handle.net/11449/17430010.1016/j.anireprosci.2017.03.0012-s2.0-850146564952-s2.0-85014656495.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Science0,704info:eu-repo/semantics/openAccess2023-10-27T06:09:41Zoai:repositorio.unesp.br:11449/174300Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-27T06:09:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes |
title |
Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes |
spellingShingle |
Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes Bartlewski, P. M. Anestrus Estradiol Follicle-stimulating hormone Sheep Superovulation |
title_short |
Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes |
title_full |
Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes |
title_fullStr |
Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes |
title_full_unstemmed |
Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes |
title_sort |
Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes |
author |
Bartlewski, P. M. |
author_facet |
Bartlewski, P. M. Murawski, M. Schwarz, T. Oliveira, M. E.F. [UNESP] |
author_role |
author |
author2 |
Murawski, M. Schwarz, T. Oliveira, M. E.F. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
University of Guelph Agricultural University of Kraków Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bartlewski, P. M. Murawski, M. Schwarz, T. Oliveira, M. E.F. [UNESP] |
dc.subject.por.fl_str_mv |
Anestrus Estradiol Follicle-stimulating hormone Sheep Superovulation |
topic |
Anestrus Estradiol Follicle-stimulating hormone Sheep Superovulation |
description |
The effect of varying intervals between successive gonadotropin injections on the superovulatory outcomes in anestrous Rideau Arcott ewes superstimulated for ovarian follicular development with multiple doses of porcine FSH (pFSH) was evaluated in a single study. Twenty-five animals received six (1 × 2.5 ml and 5 × 1.25 ml) injections of Folltropin®-V given at 0800 and 1600 h or at 0800 and 2000 h in Group 1 (n = 9) or Group 2 (n = 16), respectively. An i.m. injection of 500 IU of equine chorionic gonadotropin (eCG; Folligon®) was given concurrently with the first pFSH dose. Time of estrus was synchronized among ewes with intravaginal sponges containing 60 mg of medroxyprogesterone acetate (Veramix®) that were left in place for 14 days; sponges were removed at the time of the 5th pFSH injection. Six days after insertion of MAP sponges, all ewes received an i.m. injection of estradiol-17β dissolved in 1 ml of sesame oil (350 μg/ewe) to synchronize follicular wave emergence. Following the last pFSH dose, all animals were given a single i.m. injection of 50 μg of gonadotropin-releasing hormone (GnRH; Cystorelin®) to induce ovulations before placing in a pen with four fertile rams for 36 h. The ovarian responses were assessed and embryos recovered surgically 7 days after GnRH injections. The mean number of corpora lutea was greater (P < 0.05) in Group 1 compared with Group 2 ewes (21.0 ± 2.9 compared with 10.4 ± 1.6, respectively; mean ± SEM) but there was no difference (P > 0.05) in the number of transferable embryos (5.4 ± 2.4 compared with 5.4 ± 1.3/ewe, respectively), and Group 1 animals had significantly more degenerated embryos than Group 2 ewes (2.6 ± 1.2 compared with 0.6 ± 0.3/ewe, respectively). A superovulatory protocol wherein pFSH injections were given at 0800 and 1600 h was more effective in terms of inducing multiple ovulations than the protocol with 12-h intervals between consecutive pFSH doses, but it was not associated with an increased production of transferable quality embryos by anestrous ewes. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 2018-12-11T17:10:20Z 2018-12-11T17:10:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2017.03.001 Animal Reproduction Science, v. 180, p. 44-49. 0378-4320 http://hdl.handle.net/11449/174300 10.1016/j.anireprosci.2017.03.001 2-s2.0-85014656495 2-s2.0-85014656495.pdf |
url |
http://dx.doi.org/10.1016/j.anireprosci.2017.03.001 http://hdl.handle.net/11449/174300 |
identifier_str_mv |
Animal Reproduction Science, v. 180, p. 44-49. 0378-4320 10.1016/j.anireprosci.2017.03.001 2-s2.0-85014656495 2-s2.0-85014656495.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science 0,704 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
44-49 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964721608130560 |