Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides

Detalhes bibliográficos
Autor(a) principal: Levorato Vinche, Adriele Dandara [UNESP]
Data de Publicação: 2020
Outros Autores: de-La-Cruz-Chacón, Iván, González-Esquinca, Alma Rosa, de Fátima da Silva, Julhiany [UNESP], Ferreira, Gisela [UNESP], dos Santos, Daniela Carvalho [UNESP], Garces, Hans Garcia [UNESP], de Oliveira, Daniela Vanessa Moris, Marçon, Camila [UNESP], de Souza Cavalcante, Ricardo [UNESP], Mendes, Rinaldo Poncio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0023
http://hdl.handle.net/11449/206939
Resumo: Background: Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or noninfectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. The present study aimed to evaluate the activity of liriodenine extracted from Annona macroprophyllata on agents of systemic mycoses, with emphasis on the genus Paracoccidioides. Methods: The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined by the microdilution method. The cellular alterations caused by liriodenine on a standard P. brasiliensis (Pb18) strain were evaluated by transmission and scanning electron microscopy. Results: Liriodenine was effective only in 3 of the 8 strains of the genus Paracoccidioides and in the Histoplasma capsulatum strain, in a very low concentration (MIC of 1.95 μg.mL-1); on yeasts of Candida spp. (MIC of 125 to 250 μg.mL-1), including C. krusei (250 μg.mL-1), which has intrinsic resistance to fluconazole; and in Cryptococcus neoformans and Cryptococcus gattii (MIC of 62.5 μg.mL-1). However, liriodenine was not effective against Aspergillus fumigatus at the studied concentrations. Liriodenine exhibited fungicidal activity against all standard strains and clinical isolates that showed to be susceptible by in vitro tests. Electron microscopy revealed cytoplasmic alterations and damage to the cell wall of P. brasiliensis (Pb18). Conclusion: Our results indicate that liriodenine is a promising fungicidal compound that should undergo further investigation with some chemical modifications.
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spelling Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioidesAntifungal compoundsAntimicrobial compoundsLiriodenineMedicinal plantsParacoccidioidomycosisSystemic mycosisBackground: Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or noninfectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. The present study aimed to evaluate the activity of liriodenine extracted from Annona macroprophyllata on agents of systemic mycoses, with emphasis on the genus Paracoccidioides. Methods: The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined by the microdilution method. The cellular alterations caused by liriodenine on a standard P. brasiliensis (Pb18) strain were evaluated by transmission and scanning electron microscopy. Results: Liriodenine was effective only in 3 of the 8 strains of the genus Paracoccidioides and in the Histoplasma capsulatum strain, in a very low concentration (MIC of 1.95 μg.mL-1); on yeasts of Candida spp. (MIC of 125 to 250 μg.mL-1), including C. krusei (250 μg.mL-1), which has intrinsic resistance to fluconazole; and in Cryptococcus neoformans and Cryptococcus gattii (MIC of 62.5 μg.mL-1). However, liriodenine was not effective against Aspergillus fumigatus at the studied concentrations. Liriodenine exhibited fungicidal activity against all standard strains and clinical isolates that showed to be susceptible by in vitro tests. Electron microscopy revealed cytoplasmic alterations and damage to the cell wall of P. brasiliensis (Pb18). Conclusion: Our results indicate that liriodenine is a promising fungicidal compound that should undergo further investigation with some chemical modifications.Department of Tropical Disease and Imaging Diagnosis Botucatu Medical School São Paulo State University (UNESP)Universidad de Ciencias y Artes de ChiapasInstitute of Biosciences of Botucatu São Paulo State University (UNESP)Faculdade de Ciências da Saúde Universidade do Oeste Paulista (Unoeste)Department of Tropical Disease and Imaging Diagnosis Botucatu Medical School São Paulo State University (UNESP)Institute of Biosciences of Botucatu São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidad de Ciencias y Artes de ChiapasUniversidade do Oeste Paulista (Unoeste)Levorato Vinche, Adriele Dandara [UNESP]de-La-Cruz-Chacón, IvánGonzález-Esquinca, Alma Rosade Fátima da Silva, Julhiany [UNESP]Ferreira, Gisela [UNESP]dos Santos, Daniela Carvalho [UNESP]Garces, Hans Garcia [UNESP]de Oliveira, Daniela Vanessa MorisMarçon, Camila [UNESP]de Souza Cavalcante, Ricardo [UNESP]Mendes, Rinaldo Poncio [UNESP]2021-06-25T10:46:22Z2021-06-25T10:46:22Z2020-11-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0023Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 26.1678-91991678-9180http://hdl.handle.net/11449/20693910.1590/1678-9199-JVATITD-2020-0023S1678-919920200001003372-s2.0-85097275020S1678-91992020000100337.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-08-15T15:23:01Zoai:repositorio.unesp.br:11449/206939Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:23:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides
title Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides
spellingShingle Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides
Levorato Vinche, Adriele Dandara [UNESP]
Antifungal compounds
Antimicrobial compounds
Liriodenine
Medicinal plants
Paracoccidioidomycosis
Systemic mycosis
title_short Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides
title_full Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides
title_fullStr Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides
title_full_unstemmed Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides
title_sort Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides
author Levorato Vinche, Adriele Dandara [UNESP]
author_facet Levorato Vinche, Adriele Dandara [UNESP]
de-La-Cruz-Chacón, Iván
González-Esquinca, Alma Rosa
de Fátima da Silva, Julhiany [UNESP]
Ferreira, Gisela [UNESP]
dos Santos, Daniela Carvalho [UNESP]
Garces, Hans Garcia [UNESP]
de Oliveira, Daniela Vanessa Moris
Marçon, Camila [UNESP]
de Souza Cavalcante, Ricardo [UNESP]
Mendes, Rinaldo Poncio [UNESP]
author_role author
author2 de-La-Cruz-Chacón, Iván
González-Esquinca, Alma Rosa
de Fátima da Silva, Julhiany [UNESP]
Ferreira, Gisela [UNESP]
dos Santos, Daniela Carvalho [UNESP]
Garces, Hans Garcia [UNESP]
de Oliveira, Daniela Vanessa Moris
Marçon, Camila [UNESP]
de Souza Cavalcante, Ricardo [UNESP]
Mendes, Rinaldo Poncio [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidad de Ciencias y Artes de Chiapas
Universidade do Oeste Paulista (Unoeste)
dc.contributor.author.fl_str_mv Levorato Vinche, Adriele Dandara [UNESP]
de-La-Cruz-Chacón, Iván
González-Esquinca, Alma Rosa
de Fátima da Silva, Julhiany [UNESP]
Ferreira, Gisela [UNESP]
dos Santos, Daniela Carvalho [UNESP]
Garces, Hans Garcia [UNESP]
de Oliveira, Daniela Vanessa Moris
Marçon, Camila [UNESP]
de Souza Cavalcante, Ricardo [UNESP]
Mendes, Rinaldo Poncio [UNESP]
dc.subject.por.fl_str_mv Antifungal compounds
Antimicrobial compounds
Liriodenine
Medicinal plants
Paracoccidioidomycosis
Systemic mycosis
topic Antifungal compounds
Antimicrobial compounds
Liriodenine
Medicinal plants
Paracoccidioidomycosis
Systemic mycosis
description Background: Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or noninfectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. The present study aimed to evaluate the activity of liriodenine extracted from Annona macroprophyllata on agents of systemic mycoses, with emphasis on the genus Paracoccidioides. Methods: The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined by the microdilution method. The cellular alterations caused by liriodenine on a standard P. brasiliensis (Pb18) strain were evaluated by transmission and scanning electron microscopy. Results: Liriodenine was effective only in 3 of the 8 strains of the genus Paracoccidioides and in the Histoplasma capsulatum strain, in a very low concentration (MIC of 1.95 μg.mL-1); on yeasts of Candida spp. (MIC of 125 to 250 μg.mL-1), including C. krusei (250 μg.mL-1), which has intrinsic resistance to fluconazole; and in Cryptococcus neoformans and Cryptococcus gattii (MIC of 62.5 μg.mL-1). However, liriodenine was not effective against Aspergillus fumigatus at the studied concentrations. Liriodenine exhibited fungicidal activity against all standard strains and clinical isolates that showed to be susceptible by in vitro tests. Electron microscopy revealed cytoplasmic alterations and damage to the cell wall of P. brasiliensis (Pb18). Conclusion: Our results indicate that liriodenine is a promising fungicidal compound that should undergo further investigation with some chemical modifications.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-28
2021-06-25T10:46:22Z
2021-06-25T10:46:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0023
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 26.
1678-9199
1678-9180
http://hdl.handle.net/11449/206939
10.1590/1678-9199-JVATITD-2020-0023
S1678-91992020000100337
2-s2.0-85097275020
S1678-91992020000100337.pdf
url http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0023
http://hdl.handle.net/11449/206939
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 26.
1678-9199
1678-9180
10.1590/1678-9199-JVATITD-2020-0023
S1678-91992020000100337
2-s2.0-85097275020
S1678-91992020000100337.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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