Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0023 http://hdl.handle.net/11449/206939 |
Resumo: | Background: Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or noninfectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. The present study aimed to evaluate the activity of liriodenine extracted from Annona macroprophyllata on agents of systemic mycoses, with emphasis on the genus Paracoccidioides. Methods: The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined by the microdilution method. The cellular alterations caused by liriodenine on a standard P. brasiliensis (Pb18) strain were evaluated by transmission and scanning electron microscopy. Results: Liriodenine was effective only in 3 of the 8 strains of the genus Paracoccidioides and in the Histoplasma capsulatum strain, in a very low concentration (MIC of 1.95 μg.mL-1); on yeasts of Candida spp. (MIC of 125 to 250 μg.mL-1), including C. krusei (250 μg.mL-1), which has intrinsic resistance to fluconazole; and in Cryptococcus neoformans and Cryptococcus gattii (MIC of 62.5 μg.mL-1). However, liriodenine was not effective against Aspergillus fumigatus at the studied concentrations. Liriodenine exhibited fungicidal activity against all standard strains and clinical isolates that showed to be susceptible by in vitro tests. Electron microscopy revealed cytoplasmic alterations and damage to the cell wall of P. brasiliensis (Pb18). Conclusion: Our results indicate that liriodenine is a promising fungicidal compound that should undergo further investigation with some chemical modifications. |
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Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioidesAntifungal compoundsAntimicrobial compoundsLiriodenineMedicinal plantsParacoccidioidomycosisSystemic mycosisBackground: Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or noninfectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. The present study aimed to evaluate the activity of liriodenine extracted from Annona macroprophyllata on agents of systemic mycoses, with emphasis on the genus Paracoccidioides. Methods: The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined by the microdilution method. The cellular alterations caused by liriodenine on a standard P. brasiliensis (Pb18) strain were evaluated by transmission and scanning electron microscopy. Results: Liriodenine was effective only in 3 of the 8 strains of the genus Paracoccidioides and in the Histoplasma capsulatum strain, in a very low concentration (MIC of 1.95 μg.mL-1); on yeasts of Candida spp. (MIC of 125 to 250 μg.mL-1), including C. krusei (250 μg.mL-1), which has intrinsic resistance to fluconazole; and in Cryptococcus neoformans and Cryptococcus gattii (MIC of 62.5 μg.mL-1). However, liriodenine was not effective against Aspergillus fumigatus at the studied concentrations. Liriodenine exhibited fungicidal activity against all standard strains and clinical isolates that showed to be susceptible by in vitro tests. Electron microscopy revealed cytoplasmic alterations and damage to the cell wall of P. brasiliensis (Pb18). Conclusion: Our results indicate that liriodenine is a promising fungicidal compound that should undergo further investigation with some chemical modifications.Department of Tropical Disease and Imaging Diagnosis Botucatu Medical School São Paulo State University (UNESP)Universidad de Ciencias y Artes de ChiapasInstitute of Biosciences of Botucatu São Paulo State University (UNESP)Faculdade de Ciências da Saúde Universidade do Oeste Paulista (Unoeste)Department of Tropical Disease and Imaging Diagnosis Botucatu Medical School São Paulo State University (UNESP)Institute of Biosciences of Botucatu São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidad de Ciencias y Artes de ChiapasUniversidade do Oeste Paulista (Unoeste)Levorato Vinche, Adriele Dandara [UNESP]de-La-Cruz-Chacón, IvánGonzález-Esquinca, Alma Rosade Fátima da Silva, Julhiany [UNESP]Ferreira, Gisela [UNESP]dos Santos, Daniela Carvalho [UNESP]Garces, Hans Garcia [UNESP]de Oliveira, Daniela Vanessa MorisMarçon, Camila [UNESP]de Souza Cavalcante, Ricardo [UNESP]Mendes, Rinaldo Poncio [UNESP]2021-06-25T10:46:22Z2021-06-25T10:46:22Z2020-11-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0023Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 26.1678-91991678-9180http://hdl.handle.net/11449/20693910.1590/1678-9199-JVATITD-2020-0023S1678-919920200001003372-s2.0-85097275020S1678-91992020000100337.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-08-15T15:23:01Zoai:repositorio.unesp.br:11449/206939Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:23:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides |
title |
Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides |
spellingShingle |
Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides Levorato Vinche, Adriele Dandara [UNESP] Antifungal compounds Antimicrobial compounds Liriodenine Medicinal plants Paracoccidioidomycosis Systemic mycosis |
title_short |
Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides |
title_full |
Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides |
title_fullStr |
Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides |
title_full_unstemmed |
Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides |
title_sort |
Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus paracoccidioides |
author |
Levorato Vinche, Adriele Dandara [UNESP] |
author_facet |
Levorato Vinche, Adriele Dandara [UNESP] de-La-Cruz-Chacón, Iván González-Esquinca, Alma Rosa de Fátima da Silva, Julhiany [UNESP] Ferreira, Gisela [UNESP] dos Santos, Daniela Carvalho [UNESP] Garces, Hans Garcia [UNESP] de Oliveira, Daniela Vanessa Moris Marçon, Camila [UNESP] de Souza Cavalcante, Ricardo [UNESP] Mendes, Rinaldo Poncio [UNESP] |
author_role |
author |
author2 |
de-La-Cruz-Chacón, Iván González-Esquinca, Alma Rosa de Fátima da Silva, Julhiany [UNESP] Ferreira, Gisela [UNESP] dos Santos, Daniela Carvalho [UNESP] Garces, Hans Garcia [UNESP] de Oliveira, Daniela Vanessa Moris Marçon, Camila [UNESP] de Souza Cavalcante, Ricardo [UNESP] Mendes, Rinaldo Poncio [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidad de Ciencias y Artes de Chiapas Universidade do Oeste Paulista (Unoeste) |
dc.contributor.author.fl_str_mv |
Levorato Vinche, Adriele Dandara [UNESP] de-La-Cruz-Chacón, Iván González-Esquinca, Alma Rosa de Fátima da Silva, Julhiany [UNESP] Ferreira, Gisela [UNESP] dos Santos, Daniela Carvalho [UNESP] Garces, Hans Garcia [UNESP] de Oliveira, Daniela Vanessa Moris Marçon, Camila [UNESP] de Souza Cavalcante, Ricardo [UNESP] Mendes, Rinaldo Poncio [UNESP] |
dc.subject.por.fl_str_mv |
Antifungal compounds Antimicrobial compounds Liriodenine Medicinal plants Paracoccidioidomycosis Systemic mycosis |
topic |
Antifungal compounds Antimicrobial compounds Liriodenine Medicinal plants Paracoccidioidomycosis Systemic mycosis |
description |
Background: Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or noninfectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. The present study aimed to evaluate the activity of liriodenine extracted from Annona macroprophyllata on agents of systemic mycoses, with emphasis on the genus Paracoccidioides. Methods: The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined by the microdilution method. The cellular alterations caused by liriodenine on a standard P. brasiliensis (Pb18) strain were evaluated by transmission and scanning electron microscopy. Results: Liriodenine was effective only in 3 of the 8 strains of the genus Paracoccidioides and in the Histoplasma capsulatum strain, in a very low concentration (MIC of 1.95 μg.mL-1); on yeasts of Candida spp. (MIC of 125 to 250 μg.mL-1), including C. krusei (250 μg.mL-1), which has intrinsic resistance to fluconazole; and in Cryptococcus neoformans and Cryptococcus gattii (MIC of 62.5 μg.mL-1). However, liriodenine was not effective against Aspergillus fumigatus at the studied concentrations. Liriodenine exhibited fungicidal activity against all standard strains and clinical isolates that showed to be susceptible by in vitro tests. Electron microscopy revealed cytoplasmic alterations and damage to the cell wall of P. brasiliensis (Pb18). Conclusion: Our results indicate that liriodenine is a promising fungicidal compound that should undergo further investigation with some chemical modifications. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-28 2021-06-25T10:46:22Z 2021-06-25T10:46:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0023 Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 26. 1678-9199 1678-9180 http://hdl.handle.net/11449/206939 10.1590/1678-9199-JVATITD-2020-0023 S1678-91992020000100337 2-s2.0-85097275020 S1678-91992020000100337.pdf |
url |
http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0023 http://hdl.handle.net/11449/206939 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 26. 1678-9199 1678-9180 10.1590/1678-9199-JVATITD-2020-0023 S1678-91992020000100337 2-s2.0-85097275020 S1678-91992020000100337.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128163070345216 |