Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defects
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12903-016-0210-3 http://hdl.handle.net/11449/174030 |
Resumo: | Background: Supplementation of bone substitutes with recombinant platelet-derived growth factor-BB (PDGF-BB) can enhance bone regeneration. The aim of the study was to evaluate the effect of PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix in a rat calvaria defect model. Methods: The authors examined 5 mm rat calvarial defects treated with ß-tricalcium phosphate (TCP) or demineralized bovine bone mineral (DBBM) with and without 0.3 mg/ml recombinant PDGF-BB. Calvaria defects were randomly divided into the following treatment groups (n = 5); TCP; TCP plus PDGF-BB; DBBM; DBBM plus PDGF-BB; and untreated empty control. After 45 days, bone formation was evaluated by histomorphometry and fluorescence microscopy. Results: The authors report that the area of newly formed bone was similar between the empty controls and the two bone substitutes, TCP and DBBM. Supplementation of TCP and DBBM with PDGF-BB had no significant impact on bone formation. Fluorochrome staining revealed no visible changes in the pattern of bone formation in defects filled with TCP and DBBM, irrespective of PDGF-BB. Furthermore, supplementation with PDGF-BB did not influence biomaterial degradation. Conclusions: The authors concluded that PDGF-BB had no impact on bone formation and degradation of bone substitutes in the respective rodent models. Thus, possible beneficial effects of PDGF-BB may require other model situations. |
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Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defectsBone augmentationBone formationBone regenerationBone substitutesDemineralized bovine bone mineralGraft consolidationHistomorphometryPlatelet-derived growth factor-BBRat calvaria defectsß-tricalcium phosphateBackground: Supplementation of bone substitutes with recombinant platelet-derived growth factor-BB (PDGF-BB) can enhance bone regeneration. The aim of the study was to evaluate the effect of PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix in a rat calvaria defect model. Methods: The authors examined 5 mm rat calvarial defects treated with ß-tricalcium phosphate (TCP) or demineralized bovine bone mineral (DBBM) with and without 0.3 mg/ml recombinant PDGF-BB. Calvaria defects were randomly divided into the following treatment groups (n = 5); TCP; TCP plus PDGF-BB; DBBM; DBBM plus PDGF-BB; and untreated empty control. After 45 days, bone formation was evaluated by histomorphometry and fluorescence microscopy. Results: The authors report that the area of newly formed bone was similar between the empty controls and the two bone substitutes, TCP and DBBM. Supplementation of TCP and DBBM with PDGF-BB had no significant impact on bone formation. Fluorochrome staining revealed no visible changes in the pattern of bone formation in defects filled with TCP and DBBM, irrespective of PDGF-BB. Furthermore, supplementation with PDGF-BB did not influence biomaterial degradation. Conclusions: The authors concluded that PDGF-BB had no impact on bone formation and degradation of bone substitutes in the respective rodent models. Thus, possible beneficial effects of PDGF-BB may require other model situations.Department of Surgery and Integrated Clinic Araçatuba Dental School UNESP-Univ Estadual PaulistaKarl Donath Laboratory for Hard Tissue and Biomaterial Research Division of Oral Surgery Medical University of ViennaAustrian Cluster for Tissue RegenerationDepartment of Preventive Restorative and Pediatric Dentistry School of Dental Medicine University of BernDepartment of Oral Biology Medical University of ViennaDepartment of Anatomy Araçatuba Dental School UNESP-Univ Estadual PaulistaDepartment of Surgery and Integrated Clinic Araçatuba Dental School UNESP-Univ Estadual PaulistaDepartment of Anatomy Araçatuba Dental School UNESP-Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)Medical University of ViennaAustrian Cluster for Tissue RegenerationUniversity of BernLuvizuto, Eloá R. [UNESP]Tangl, StefanDobsak, ToniReich, KarolineGruber, ReinhardSonoda, Celso K. [UNESP]Okamoto, Roberta [UNESP]2018-12-11T17:08:48Z2018-12-11T17:08:48Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7application/pdfhttp://dx.doi.org/10.1186/s12903-016-0210-3BMC Oral Health, v. 16, n. 1, p. 1-7, 2016.1472-6831http://hdl.handle.net/11449/17403010.1186/s12903-016-0210-32-s2.0-850083393542-s2.0-85008339354.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Oral Health0,867info:eu-repo/semantics/openAccess2023-10-22T06:11:11Zoai:repositorio.unesp.br:11449/174030Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:39:01.622682Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defects |
title |
Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defects |
spellingShingle |
Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defects Luvizuto, Eloá R. [UNESP] Bone augmentation Bone formation Bone regeneration Bone substitutes Demineralized bovine bone mineral Graft consolidation Histomorphometry Platelet-derived growth factor-BB Rat calvaria defects ß-tricalcium phosphate |
title_short |
Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defects |
title_full |
Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defects |
title_fullStr |
Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defects |
title_full_unstemmed |
Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defects |
title_sort |
Effect of recombinant PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix: A pilot study in rat calvarial defects |
author |
Luvizuto, Eloá R. [UNESP] |
author_facet |
Luvizuto, Eloá R. [UNESP] Tangl, Stefan Dobsak, Toni Reich, Karoline Gruber, Reinhard Sonoda, Celso K. [UNESP] Okamoto, Roberta [UNESP] |
author_role |
author |
author2 |
Tangl, Stefan Dobsak, Toni Reich, Karoline Gruber, Reinhard Sonoda, Celso K. [UNESP] Okamoto, Roberta [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Medical University of Vienna Austrian Cluster for Tissue Regeneration University of Bern |
dc.contributor.author.fl_str_mv |
Luvizuto, Eloá R. [UNESP] Tangl, Stefan Dobsak, Toni Reich, Karoline Gruber, Reinhard Sonoda, Celso K. [UNESP] Okamoto, Roberta [UNESP] |
dc.subject.por.fl_str_mv |
Bone augmentation Bone formation Bone regeneration Bone substitutes Demineralized bovine bone mineral Graft consolidation Histomorphometry Platelet-derived growth factor-BB Rat calvaria defects ß-tricalcium phosphate |
topic |
Bone augmentation Bone formation Bone regeneration Bone substitutes Demineralized bovine bone mineral Graft consolidation Histomorphometry Platelet-derived growth factor-BB Rat calvaria defects ß-tricalcium phosphate |
description |
Background: Supplementation of bone substitutes with recombinant platelet-derived growth factor-BB (PDGF-BB) can enhance bone regeneration. The aim of the study was to evaluate the effect of PDGF-BB on bone formation in the presence of ß-tricalcium phosphate and bovine bone mineral matrix in a rat calvaria defect model. Methods: The authors examined 5 mm rat calvarial defects treated with ß-tricalcium phosphate (TCP) or demineralized bovine bone mineral (DBBM) with and without 0.3 mg/ml recombinant PDGF-BB. Calvaria defects were randomly divided into the following treatment groups (n = 5); TCP; TCP plus PDGF-BB; DBBM; DBBM plus PDGF-BB; and untreated empty control. After 45 days, bone formation was evaluated by histomorphometry and fluorescence microscopy. Results: The authors report that the area of newly formed bone was similar between the empty controls and the two bone substitutes, TCP and DBBM. Supplementation of TCP and DBBM with PDGF-BB had no significant impact on bone formation. Fluorochrome staining revealed no visible changes in the pattern of bone formation in defects filled with TCP and DBBM, irrespective of PDGF-BB. Furthermore, supplementation with PDGF-BB did not influence biomaterial degradation. Conclusions: The authors concluded that PDGF-BB had no impact on bone formation and degradation of bone substitutes in the respective rodent models. Thus, possible beneficial effects of PDGF-BB may require other model situations. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:08:48Z 2018-12-11T17:08:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12903-016-0210-3 BMC Oral Health, v. 16, n. 1, p. 1-7, 2016. 1472-6831 http://hdl.handle.net/11449/174030 10.1186/s12903-016-0210-3 2-s2.0-85008339354 2-s2.0-85008339354.pdf |
url |
http://dx.doi.org/10.1186/s12903-016-0210-3 http://hdl.handle.net/11449/174030 |
identifier_str_mv |
BMC Oral Health, v. 16, n. 1, p. 1-7, 2016. 1472-6831 10.1186/s12903-016-0210-3 2-s2.0-85008339354 2-s2.0-85008339354.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Oral Health 0,867 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-7 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128545193459712 |