Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal species
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1099/jmm.0.001575 http://hdl.handle.net/11449/247639 |
Resumo: | Introduction. Plants have been used as medicines for centuries to treat human diseases. Studies with plants are extremely important for the development of future drugs that can benefit the human population. Hypothesis/Gap Statement. With the emergence of pathogens resistant to antimicrobial agents, there is an urgent need to direct research towards the discovery of new antimicrobials. Aim. In this study, Spondias purpurea L. (Anacardiaceae) was evaluated for its antimicrobial activity, antioxidant activity and cytotoxicity. Methodology. Antimicrobial activity was evaluated by the MIC using the 96-well plate microdilution technique of ethanolic, hexanic and dicloromethanic extracts of dried S. purpurea leaves against bacteria, yeast and filamentous fungi. The antioxidant activity of extracts was evaluated by the 2, 2-diphenyl-1-picrylhydrazine (DPPH) method. To evaluate the safety of extracts, a cytotoxicity study against HaCat, J774 and HepG2 cells was performed. Results. The extracts had no activity against the bacteria at the maximum concentration of 5.0 mg ml−1, but showed fungistatic action against Candida species and dermatophytes. The ethanolic extract showed 88% antioxidant activity and showed no significant cytotoxicity against the previously mentioned cells. Conclusion. This study showed that the 100% ethanolic (EtOH) extract was favourable for antifungal and antioxidant activities and did not present significant cytotoxicity against the three studied cell lines, indicating that S. purpurea leaves are promising for the development of new antimicrobials. |
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Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal speciesAnacardiaceaeantimicrobial activityantioxidant activitycytotoxicityseriguelaSpondias purpureaIntroduction. Plants have been used as medicines for centuries to treat human diseases. Studies with plants are extremely important for the development of future drugs that can benefit the human population. Hypothesis/Gap Statement. With the emergence of pathogens resistant to antimicrobial agents, there is an urgent need to direct research towards the discovery of new antimicrobials. Aim. In this study, Spondias purpurea L. (Anacardiaceae) was evaluated for its antimicrobial activity, antioxidant activity and cytotoxicity. Methodology. Antimicrobial activity was evaluated by the MIC using the 96-well plate microdilution technique of ethanolic, hexanic and dicloromethanic extracts of dried S. purpurea leaves against bacteria, yeast and filamentous fungi. The antioxidant activity of extracts was evaluated by the 2, 2-diphenyl-1-picrylhydrazine (DPPH) method. To evaluate the safety of extracts, a cytotoxicity study against HaCat, J774 and HepG2 cells was performed. Results. The extracts had no activity against the bacteria at the maximum concentration of 5.0 mg ml−1, but showed fungistatic action against Candida species and dermatophytes. The ethanolic extract showed 88% antioxidant activity and showed no significant cytotoxicity against the previously mentioned cells. Conclusion. This study showed that the 100% ethanolic (EtOH) extract was favourable for antifungal and antioxidant activities and did not present significant cytotoxicity against the three studied cell lines, indicating that S. purpurea leaves are promising for the development of new antimicrobials.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Skaggs School of Pharmacy and Pharmaceutical SciencesDepartment of Drugs and Medicines School of Pharmaceutical Sciences of Araraquara São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, SPDepartment of Drugs and Medicines School of Pharmaceutical Sciences of Araraquara São Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 1, SPUniversidade Estadual Paulista (UNESP)de Oliveira, Thais Emboaba [UNESP]Greatti, Vanessa Raquel [UNESP]Sorrechia, Rodrigo [UNESP]Pietro, Rosemeire C.L.R. [UNESP]2023-07-29T13:21:46Z2023-07-29T13:21:46Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1099/jmm.0.001575Journal of Medical Microbiology, v. 71, n. 9, 2022.1473-56440022-2615http://hdl.handle.net/11449/24763910.1099/jmm.0.0015752-s2.0-85138446399Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Medical Microbiologyinfo:eu-repo/semantics/openAccess2024-06-24T13:44:57Zoai:repositorio.unesp.br:11449/247639Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:14:52.558159Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal species |
title |
Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal species |
spellingShingle |
Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal species de Oliveira, Thais Emboaba [UNESP] Anacardiaceae antimicrobial activity antioxidant activity cytotoxicity seriguela Spondias purpurea |
title_short |
Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal species |
title_full |
Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal species |
title_fullStr |
Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal species |
title_full_unstemmed |
Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal species |
title_sort |
Antimicrobial activity: potential of Spondias purpurea (Anacardiaceae) against bacterial and fungal species |
author |
de Oliveira, Thais Emboaba [UNESP] |
author_facet |
de Oliveira, Thais Emboaba [UNESP] Greatti, Vanessa Raquel [UNESP] Sorrechia, Rodrigo [UNESP] Pietro, Rosemeire C.L.R. [UNESP] |
author_role |
author |
author2 |
Greatti, Vanessa Raquel [UNESP] Sorrechia, Rodrigo [UNESP] Pietro, Rosemeire C.L.R. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
de Oliveira, Thais Emboaba [UNESP] Greatti, Vanessa Raquel [UNESP] Sorrechia, Rodrigo [UNESP] Pietro, Rosemeire C.L.R. [UNESP] |
dc.subject.por.fl_str_mv |
Anacardiaceae antimicrobial activity antioxidant activity cytotoxicity seriguela Spondias purpurea |
topic |
Anacardiaceae antimicrobial activity antioxidant activity cytotoxicity seriguela Spondias purpurea |
description |
Introduction. Plants have been used as medicines for centuries to treat human diseases. Studies with plants are extremely important for the development of future drugs that can benefit the human population. Hypothesis/Gap Statement. With the emergence of pathogens resistant to antimicrobial agents, there is an urgent need to direct research towards the discovery of new antimicrobials. Aim. In this study, Spondias purpurea L. (Anacardiaceae) was evaluated for its antimicrobial activity, antioxidant activity and cytotoxicity. Methodology. Antimicrobial activity was evaluated by the MIC using the 96-well plate microdilution technique of ethanolic, hexanic and dicloromethanic extracts of dried S. purpurea leaves against bacteria, yeast and filamentous fungi. The antioxidant activity of extracts was evaluated by the 2, 2-diphenyl-1-picrylhydrazine (DPPH) method. To evaluate the safety of extracts, a cytotoxicity study against HaCat, J774 and HepG2 cells was performed. Results. The extracts had no activity against the bacteria at the maximum concentration of 5.0 mg ml−1, but showed fungistatic action against Candida species and dermatophytes. The ethanolic extract showed 88% antioxidant activity and showed no significant cytotoxicity against the previously mentioned cells. Conclusion. This study showed that the 100% ethanolic (EtOH) extract was favourable for antifungal and antioxidant activities and did not present significant cytotoxicity against the three studied cell lines, indicating that S. purpurea leaves are promising for the development of new antimicrobials. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-07-29T13:21:46Z 2023-07-29T13:21:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1099/jmm.0.001575 Journal of Medical Microbiology, v. 71, n. 9, 2022. 1473-5644 0022-2615 http://hdl.handle.net/11449/247639 10.1099/jmm.0.001575 2-s2.0-85138446399 |
url |
http://dx.doi.org/10.1099/jmm.0.001575 http://hdl.handle.net/11449/247639 |
identifier_str_mv |
Journal of Medical Microbiology, v. 71, n. 9, 2022. 1473-5644 0022-2615 10.1099/jmm.0.001575 2-s2.0-85138446399 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Medical Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128336132571136 |