Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalis
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4081%2Fijas.2007.s2.287 http://www.aspajournal.it/index.php/ijas/article/view/ijas.2007.s2.287 http://hdl.handle.net/11449/38252 |
Resumo: | Conjugated Linoleic Acids (CLAs) comprise a family of positional and geometric isomers of linoleic acid. The main form of CLA, cis-9, trans-11-C18:2 show positive effects in cancer prevention and treatment. The major dietary sources of these fatty acids are derived from ruminant animals, in particular dairy products. In these animals, the endogenous synthesis mainly occurs in mammary gland by the action of enzyme Stearoyl CoA Desaturase (SCD). Different levels of expression and activity of SCD in mammary gland can explain partially the variation of CLA levels in fat milk. Considering a great fat concentration in bubaline milk and the benefit of a high and positive correlation between fat milk and CLA production, this study was carried on with the intention of sequencing and characterizing part of the gene that codifies SCD in buffaloes. Genomic DNA was extracted from blood samples of lactating bubaline which begins to the breed Murrah. After the (acho que nao precisa desse the) extractions, PCR (Polymerase Chain Reaction) reactions were made by using primers Z (sic) (sic) D1 and E1 (sic) (sic) F1. The fragments obtained in PCR were cloned into T vectors and transformed in competent cells DH10B line. After this, three samples of each fragment were sequenced from 5' and 3' extremities using a BigDye kit in an automatic sequencer. Sequences were edited in a consensus of each fragment and were submitted to BLAST-n / NCBI for similarity comparisions among other species. The sequence obtained with Z (sic) (sic) D1 primers shows 938 bp enclosing exons 1 and 2 and intron 1. The primers E1 (sic) (sic) F1 show 70 bp corresponding to exon 3 of bubaline SCD gene. Similarities were obtained between 85% and 97% among bubaline sequences and sequences of SCD gene described in human, mouse, rat, swine, bovine, caprine and ovine species. This study has permitted the identification and partial characterization of SCD codifing region in Bubalus bubalis specie. |
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Repositório Institucional da UNESP |
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Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalisMilkCLAPCRGenomic DNA sequenceConjugated Linoleic Acids (CLAs) comprise a family of positional and geometric isomers of linoleic acid. The main form of CLA, cis-9, trans-11-C18:2 show positive effects in cancer prevention and treatment. The major dietary sources of these fatty acids are derived from ruminant animals, in particular dairy products. In these animals, the endogenous synthesis mainly occurs in mammary gland by the action of enzyme Stearoyl CoA Desaturase (SCD). Different levels of expression and activity of SCD in mammary gland can explain partially the variation of CLA levels in fat milk. Considering a great fat concentration in bubaline milk and the benefit of a high and positive correlation between fat milk and CLA production, this study was carried on with the intention of sequencing and characterizing part of the gene that codifies SCD in buffaloes. Genomic DNA was extracted from blood samples of lactating bubaline which begins to the breed Murrah. After the (acho que nao precisa desse the) extractions, PCR (Polymerase Chain Reaction) reactions were made by using primers Z (sic) (sic) D1 and E1 (sic) (sic) F1. The fragments obtained in PCR were cloned into T vectors and transformed in competent cells DH10B line. After this, three samples of each fragment were sequenced from 5' and 3' extremities using a BigDye kit in an automatic sequencer. Sequences were edited in a consensus of each fragment and were submitted to BLAST-n / NCBI for similarity comparisions among other species. The sequence obtained with Z (sic) (sic) D1 primers shows 938 bp enclosing exons 1 and 2 and intron 1. The primers E1 (sic) (sic) F1 show 70 bp corresponding to exon 3 of bubaline SCD gene. Similarities were obtained between 85% and 97% among bubaline sequences and sequences of SCD gene described in human, mouse, rat, swine, bovine, caprine and ovine species. This study has permitted the identification and partial characterization of SCD codifing region in Bubalus bubalis specie.São Paulo State Univ, FCAV Unesp Jaboticabal, BR-14884900 São Paulo, BrazilSão Paulo State Univ, FCAV Unesp Jaboticabal, BR-14884900 São Paulo, BrazilPagepress PublUniversidade Estadual Paulista (Unesp)Lima, A. L. F. [UNESP]Otaviano, A. R. [UNESP]Laureano, M. M. M. [UNESP]Galeazzi, P. M. [UNESP]de Camargo, G. M. F. [UNESP]Thomazine, R. B. [UNESP]Tonhati, Humberto [UNESP]2014-05-20T15:28:27Z2014-05-20T15:28:27Z2007-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article287-290application/pdfhttp://dx.doi.org/10.4081%2Fijas.2007.s2.287http://www.aspajournal.it/index.php/ijas/article/view/ijas.2007.s2.287Italian Journal of Animal Science. Pavia: Pagepress Publ, v. 6, p. 287-290, 2007.1594-4077http://hdl.handle.net/11449/3825210.4081%2Fijas.2007.s2.287WOS:000207598400036WOS000207598400036.pdf7445254960858159Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengItalian Journal of Animal Science0.9900,434info:eu-repo/semantics/openAccess2024-01-06T06:25:29Zoai:repositorio.unesp.br:11449/38252Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-06T06:25:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalis |
title |
Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalis |
spellingShingle |
Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalis Lima, A. L. F. [UNESP] Milk CLA PCR Genomic DNA sequence |
title_short |
Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalis |
title_full |
Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalis |
title_fullStr |
Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalis |
title_full_unstemmed |
Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalis |
title_sort |
Partial genetic characterization of Stearoyl Coa-Desaturase's structural region in Bubalus bubalis |
author |
Lima, A. L. F. [UNESP] |
author_facet |
Lima, A. L. F. [UNESP] Otaviano, A. R. [UNESP] Laureano, M. M. M. [UNESP] Galeazzi, P. M. [UNESP] de Camargo, G. M. F. [UNESP] Thomazine, R. B. [UNESP] Tonhati, Humberto [UNESP] |
author_role |
author |
author2 |
Otaviano, A. R. [UNESP] Laureano, M. M. M. [UNESP] Galeazzi, P. M. [UNESP] de Camargo, G. M. F. [UNESP] Thomazine, R. B. [UNESP] Tonhati, Humberto [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lima, A. L. F. [UNESP] Otaviano, A. R. [UNESP] Laureano, M. M. M. [UNESP] Galeazzi, P. M. [UNESP] de Camargo, G. M. F. [UNESP] Thomazine, R. B. [UNESP] Tonhati, Humberto [UNESP] |
dc.subject.por.fl_str_mv |
Milk CLA PCR Genomic DNA sequence |
topic |
Milk CLA PCR Genomic DNA sequence |
description |
Conjugated Linoleic Acids (CLAs) comprise a family of positional and geometric isomers of linoleic acid. The main form of CLA, cis-9, trans-11-C18:2 show positive effects in cancer prevention and treatment. The major dietary sources of these fatty acids are derived from ruminant animals, in particular dairy products. In these animals, the endogenous synthesis mainly occurs in mammary gland by the action of enzyme Stearoyl CoA Desaturase (SCD). Different levels of expression and activity of SCD in mammary gland can explain partially the variation of CLA levels in fat milk. Considering a great fat concentration in bubaline milk and the benefit of a high and positive correlation between fat milk and CLA production, this study was carried on with the intention of sequencing and characterizing part of the gene that codifies SCD in buffaloes. Genomic DNA was extracted from blood samples of lactating bubaline which begins to the breed Murrah. After the (acho que nao precisa desse the) extractions, PCR (Polymerase Chain Reaction) reactions were made by using primers Z (sic) (sic) D1 and E1 (sic) (sic) F1. The fragments obtained in PCR were cloned into T vectors and transformed in competent cells DH10B line. After this, three samples of each fragment were sequenced from 5' and 3' extremities using a BigDye kit in an automatic sequencer. Sequences were edited in a consensus of each fragment and were submitted to BLAST-n / NCBI for similarity comparisions among other species. The sequence obtained with Z (sic) (sic) D1 primers shows 938 bp enclosing exons 1 and 2 and intron 1. The primers E1 (sic) (sic) F1 show 70 bp corresponding to exon 3 of bubaline SCD gene. Similarities were obtained between 85% and 97% among bubaline sequences and sequences of SCD gene described in human, mouse, rat, swine, bovine, caprine and ovine species. This study has permitted the identification and partial characterization of SCD codifing region in Bubalus bubalis specie. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 2014-05-20T15:28:27Z 2014-05-20T15:28:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4081%2Fijas.2007.s2.287 http://www.aspajournal.it/index.php/ijas/article/view/ijas.2007.s2.287 Italian Journal of Animal Science. Pavia: Pagepress Publ, v. 6, p. 287-290, 2007. 1594-4077 http://hdl.handle.net/11449/38252 10.4081%2Fijas.2007.s2.287 WOS:000207598400036 WOS000207598400036.pdf 7445254960858159 |
url |
http://dx.doi.org/10.4081%2Fijas.2007.s2.287 http://www.aspajournal.it/index.php/ijas/article/view/ijas.2007.s2.287 http://hdl.handle.net/11449/38252 |
identifier_str_mv |
Italian Journal of Animal Science. Pavia: Pagepress Publ, v. 6, p. 287-290, 2007. 1594-4077 10.4081%2Fijas.2007.s2.287 WOS:000207598400036 WOS000207598400036.pdf 7445254960858159 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Italian Journal of Animal Science 0.990 0,434 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
287-290 application/pdf |
dc.publisher.none.fl_str_mv |
Pagepress Publ |
publisher.none.fl_str_mv |
Pagepress Publ |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965533888577536 |