Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0104-79302001000200010 http://hdl.handle.net/11449/212348 |
Resumo: | The aim of this study was to compare individual venom samples of Crotalus durissus terrificus recently captured in the wild to evaluate possible differences in venom protein composition. Protein levels were quantified by biochemical method (Biuret) and then submitted to electrophoresis. Electrophoresis studies of native protein were performed in vertical slabs of polyacrylamide gel (PAGE), in an alkaline discontinuous buffer system, with a concentration of 10% in the separation gel. SDS-PAGE was performed in PhastGel® (8-25). Both gels were stained with Coomassie Blue. Gels were analyzed using the VDS-Pharmacia® device. Our results indicate that all analyzed venom samples showed different protein composition, although common protein fractions were detected in some individual samples. Differences were observed between the different individual venom samples and so in the same specimen in relation to the time of collection, for both techniques used. Diet did not influence the variability of venom composition. There is a significant difference in native venom protein composition of males and females. |
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Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakesCrotalus durissus terrificusintraspecific variationprotein compositionvenomsexfeedingelectrophoresisThe aim of this study was to compare individual venom samples of Crotalus durissus terrificus recently captured in the wild to evaluate possible differences in venom protein composition. Protein levels were quantified by biochemical method (Biuret) and then submitted to electrophoresis. Electrophoresis studies of native protein were performed in vertical slabs of polyacrylamide gel (PAGE), in an alkaline discontinuous buffer system, with a concentration of 10% in the separation gel. SDS-PAGE was performed in PhastGel® (8-25). Both gels were stained with Coomassie Blue. Gels were analyzed using the VDS-Pharmacia® device. Our results indicate that all analyzed venom samples showed different protein composition, although common protein fractions were detected in some individual samples. Differences were observed between the different individual venom samples and so in the same specimen in relation to the time of collection, for both techniques used. Diet did not influence the variability of venom composition. There is a significant difference in native venom protein composition of males and females.Universidade Estadual Paulista, Instituto de BiociênciasUniversidade Estadual Paulista, Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual Paulista, Centro de Estudos de Venenos e Animais PeçonhentosUniversidade Estadual Paulista, Instituto de BiociênciasUniversidade Estadual Paulista, Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual Paulista, Centro de Estudos de Venenos e Animais PeçonhentosCentro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESPUniversidade Estadual Paulista (Unesp)Magro, A. J. [UNESP]Silva, R. J. Da [UNESP]Ramos, P. R. R. [UNESP]Cherubini, A. L. [UNESP]Hatayde, M. R. [UNESP]2021-07-14T10:38:33Z2021-07-14T10:38:33Z2001-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article276-301http://dx.doi.org/10.1590/S0104-79302001000200010Journal of Venomous Animals and Toxins. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 7, n. 2, p. 276-301, 2001.0104-79301678-4936http://hdl.handle.net/11449/21234810.1590/S0104-79302001000200010S0104-79302001000200010S0104-79302001000200010.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxinsinfo:eu-repo/semantics/openAccess2024-04-11T15:28:26Zoai:repositorio.unesp.br:11449/212348Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-11T15:28:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes |
title |
Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes |
spellingShingle |
Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes Magro, A. J. [UNESP] Crotalus durissus terrificus intraspecific variation protein composition venom sex feeding electrophoresis |
title_short |
Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes |
title_full |
Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes |
title_fullStr |
Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes |
title_full_unstemmed |
Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes |
title_sort |
Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes |
author |
Magro, A. J. [UNESP] |
author_facet |
Magro, A. J. [UNESP] Silva, R. J. Da [UNESP] Ramos, P. R. R. [UNESP] Cherubini, A. L. [UNESP] Hatayde, M. R. [UNESP] |
author_role |
author |
author2 |
Silva, R. J. Da [UNESP] Ramos, P. R. R. [UNESP] Cherubini, A. L. [UNESP] Hatayde, M. R. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Magro, A. J. [UNESP] Silva, R. J. Da [UNESP] Ramos, P. R. R. [UNESP] Cherubini, A. L. [UNESP] Hatayde, M. R. [UNESP] |
dc.subject.por.fl_str_mv |
Crotalus durissus terrificus intraspecific variation protein composition venom sex feeding electrophoresis |
topic |
Crotalus durissus terrificus intraspecific variation protein composition venom sex feeding electrophoresis |
description |
The aim of this study was to compare individual venom samples of Crotalus durissus terrificus recently captured in the wild to evaluate possible differences in venom protein composition. Protein levels were quantified by biochemical method (Biuret) and then submitted to electrophoresis. Electrophoresis studies of native protein were performed in vertical slabs of polyacrylamide gel (PAGE), in an alkaline discontinuous buffer system, with a concentration of 10% in the separation gel. SDS-PAGE was performed in PhastGel® (8-25). Both gels were stained with Coomassie Blue. Gels were analyzed using the VDS-Pharmacia® device. Our results indicate that all analyzed venom samples showed different protein composition, although common protein fractions were detected in some individual samples. Differences were observed between the different individual venom samples and so in the same specimen in relation to the time of collection, for both techniques used. Diet did not influence the variability of venom composition. There is a significant difference in native venom protein composition of males and females. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12 2021-07-14T10:38:33Z 2021-07-14T10:38:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0104-79302001000200010 Journal of Venomous Animals and Toxins. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 7, n. 2, p. 276-301, 2001. 0104-7930 1678-4936 http://hdl.handle.net/11449/212348 10.1590/S0104-79302001000200010 S0104-79302001000200010 S0104-79302001000200010.pdf |
url |
http://dx.doi.org/10.1590/S0104-79302001000200010 http://hdl.handle.net/11449/212348 |
identifier_str_mv |
Journal of Venomous Animals and Toxins. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 7, n. 2, p. 276-301, 2001. 0104-7930 1678-4936 10.1590/S0104-79302001000200010 S0104-79302001000200010 S0104-79302001000200010.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
276-301 |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965620430700544 |