The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.virol.2011.01.026 http://hdl.handle.net/11449/13095 |
Resumo: | KSHV LANA1, a latent protein expressed during chronic infection to maintain a viral genome, inhibits major histocompatibility complex class I (MHCI) peptide presentation in cis as a means of immune evasion. Through deletional cloning, we localized this function to the LANA1 central repeat 1 (CR1) subregion. Other CR subregions retard LANA1 translation and proteasomal processing but do not markedly inhibit LANA1 peptide processing by MHC I. Inhibition of proteasomal processing ablates LANA1 peptide presentation. Direct expression of LANA1 within the endoplasmic reticulum (ER) overcomes CR1 inhibition suggesting that CR1 acts prior to translocation of cytoplasmic peptides into the ER. By physically separating CR1 from other subdomains, we show that LANA1 evades MHC I peptide processing by a mechanism distinct from other herpesviruses including Epstein-Barr virus (EBV). Although LANA1 and EBV EBNA1 are functionally similar, they appear to use different mechanisms to evade host cytotoxic T lymphocyte surveillance. (C) 2011 Elsevier B.V. All rights reserved. |
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The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentationKaposi's sarcoma-associated herpesvirusLatency-associated nuclear antigen 1Major histocompatibility complex class IImmune evasionKSHV LANA1, a latent protein expressed during chronic infection to maintain a viral genome, inhibits major histocompatibility complex class I (MHCI) peptide presentation in cis as a means of immune evasion. Through deletional cloning, we localized this function to the LANA1 central repeat 1 (CR1) subregion. Other CR subregions retard LANA1 translation and proteasomal processing but do not markedly inhibit LANA1 peptide processing by MHC I. Inhibition of proteasomal processing ablates LANA1 peptide presentation. Direct expression of LANA1 within the endoplasmic reticulum (ER) overcomes CR1 inhibition suggesting that CR1 acts prior to translocation of cytoplasmic peptides into the ER. By physically separating CR1 from other subdomains, we show that LANA1 evades MHC I peptide processing by a mechanism distinct from other herpesviruses including Epstein-Barr virus (EBV). Although LANA1 and EBV EBNA1 are functionally similar, they appear to use different mechanisms to evade host cytotoxic T lymphocyte surveillance. (C) 2011 Elsevier B.V. All rights reserved.NIHCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)American Cancer SocietyUPCI Core Facility Cancer CenterUniv Pittsburgh, Inst Canc, Canc Virol Program, Pittsburgh, PA 15213 USASão Paulo State Univ, Botucatu Sch Med, Dept Pathol, São Paulo, BrazilUniv British Columbia, British Columbia Childrens Hosp, Dept Pathol & Lab Med, Vancouver, BC V5Z 1M9, CanadaUniv Pittsburgh, Med Ctr, Dept Otolaryngol, Pittsburgh, PA 15213 USAUniv Pittsburgh, Med Ctr, Dept Immunol, Pittsburgh, PA 15213 USAUniv Pittsburgh, Inst Canc, Canc Immunol Program, Pittsburgh, PA 15213 USASão Paulo State Univ, Botucatu Sch Med, Dept Pathol, São Paulo, BrazilNIH: NCI121930CAPES: BEX1049/05-4UPCI: P30 CA047904Academic Press Inc. Elsevier B.V.Univ PittsburghUniversidade Estadual Paulista (Unesp)Univ British ColumbiaKwun, Hyun Jinda Silva, Suzane Ramos [UNESP]Qin, HuilianFerris, Robert L.Tan, RusungChang, YuanMoore, Patrick S.2014-05-20T13:37:48Z2014-05-20T13:37:48Z2011-04-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article357-365application/pdfhttp://dx.doi.org/10.1016/j.virol.2011.01.026Virology. San Diego: Academic Press Inc. Elsevier B.V., v. 412, n. 2, p. 357-365, 2011.0042-6822http://hdl.handle.net/11449/1309510.1016/j.virol.2011.01.026WOS:000288773100013WOS000288773100013.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirology3.3741,728info:eu-repo/semantics/openAccess2024-09-03T13:14:07Zoai:repositorio.unesp.br:11449/13095Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation |
title |
The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation |
spellingShingle |
The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation Kwun, Hyun Jin Kaposi's sarcoma-associated herpesvirus Latency-associated nuclear antigen 1 Major histocompatibility complex class I Immune evasion |
title_short |
The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation |
title_full |
The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation |
title_fullStr |
The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation |
title_full_unstemmed |
The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation |
title_sort |
The central repeat domain 1 of Kaposi's sarcoma-associated herpesvirus (KSHV) latency associated-nuclear antigen 1 (LANA1) prevents cis MHC class I peptide presentation |
author |
Kwun, Hyun Jin |
author_facet |
Kwun, Hyun Jin da Silva, Suzane Ramos [UNESP] Qin, Huilian Ferris, Robert L. Tan, Rusung Chang, Yuan Moore, Patrick S. |
author_role |
author |
author2 |
da Silva, Suzane Ramos [UNESP] Qin, Huilian Ferris, Robert L. Tan, Rusung Chang, Yuan Moore, Patrick S. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Pittsburgh Universidade Estadual Paulista (Unesp) Univ British Columbia |
dc.contributor.author.fl_str_mv |
Kwun, Hyun Jin da Silva, Suzane Ramos [UNESP] Qin, Huilian Ferris, Robert L. Tan, Rusung Chang, Yuan Moore, Patrick S. |
dc.subject.por.fl_str_mv |
Kaposi's sarcoma-associated herpesvirus Latency-associated nuclear antigen 1 Major histocompatibility complex class I Immune evasion |
topic |
Kaposi's sarcoma-associated herpesvirus Latency-associated nuclear antigen 1 Major histocompatibility complex class I Immune evasion |
description |
KSHV LANA1, a latent protein expressed during chronic infection to maintain a viral genome, inhibits major histocompatibility complex class I (MHCI) peptide presentation in cis as a means of immune evasion. Through deletional cloning, we localized this function to the LANA1 central repeat 1 (CR1) subregion. Other CR subregions retard LANA1 translation and proteasomal processing but do not markedly inhibit LANA1 peptide processing by MHC I. Inhibition of proteasomal processing ablates LANA1 peptide presentation. Direct expression of LANA1 within the endoplasmic reticulum (ER) overcomes CR1 inhibition suggesting that CR1 acts prior to translocation of cytoplasmic peptides into the ER. By physically separating CR1 from other subdomains, we show that LANA1 evades MHC I peptide processing by a mechanism distinct from other herpesviruses including Epstein-Barr virus (EBV). Although LANA1 and EBV EBNA1 are functionally similar, they appear to use different mechanisms to evade host cytotoxic T lymphocyte surveillance. (C) 2011 Elsevier B.V. All rights reserved. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-04-10 2014-05-20T13:37:48Z 2014-05-20T13:37:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.virol.2011.01.026 Virology. San Diego: Academic Press Inc. Elsevier B.V., v. 412, n. 2, p. 357-365, 2011. 0042-6822 http://hdl.handle.net/11449/13095 10.1016/j.virol.2011.01.026 WOS:000288773100013 WOS000288773100013.pdf |
url |
http://dx.doi.org/10.1016/j.virol.2011.01.026 http://hdl.handle.net/11449/13095 |
identifier_str_mv |
Virology. San Diego: Academic Press Inc. Elsevier B.V., v. 412, n. 2, p. 357-365, 2011. 0042-6822 10.1016/j.virol.2011.01.026 WOS:000288773100013 WOS000288773100013.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Virology 3.374 1,728 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
357-365 application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc. Elsevier B.V. |
publisher.none.fl_str_mv |
Academic Press Inc. Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021355498766336 |