Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

Detalhes bibliográficos
Autor(a) principal: Castro Marcondes, Joao Paulo de [UNESP]
Data de Publicação: 2014
Outros Autores: Oliveira, Maria Luiza Cotrim Sartor de [UNESP], Gontijo, Alisson M., Camargo, João Lauro Viana de [UNESP], Salvadori, Daisy Maria Favero [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0086162
http://hdl.handle.net/11449/111191
Resumo: Bladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify individuals with asymptomatic disease. In this context, we used the micronucleus assay to evaluate whether cytogenetic alterations could be used as biomarkers for monitoring patients with a history of urothelial cell carcinoma (UCC). We determined the frequency of micronucleated urothelial cells (MNC) in exfoliated bladder cells from 105 patients with (n = 52) or without (n = 53) a history of UCC, all of whom tested negative for neoplasia by cytopathological and histopathological analyses. MNC frequencies were increased in patients with a history of UCC (non-smoker and smoker/ex-smoker patients vs non-smoker and smoker/ex-smoker controls; p<0.001), in non-smoker UCC patients (vs non-smoker controls; p<0.01), and in smoker/ex-smoker controls (vs non-smoker controls; p<0.001). Patients with a history of recurrent disease also demonstrated a higher MNC frequency compared to patients with non-recurrent neoplasia. However, logistic regression using smoking habits, age and gender as confounding factors did not confirm MNC frequency as a marker for UCC recurrence. Fluorescent in situ hybridization analysis (using a pan-centromeric probe) showed that micronuclei (MN) arose mainly from clastogenic events regardless of UCC and/or smoking histories. In conclusion, our results confirm previous indications that subjects with a history of UCC harbor genetically unstable cells in the bladder urothelium. Furthermore, these results support using the micronucleus assay as an important tool for monitoring patients with a history of UCC and tumor recurrence.
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spelling Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell CarcinomaBladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify individuals with asymptomatic disease. In this context, we used the micronucleus assay to evaluate whether cytogenetic alterations could be used as biomarkers for monitoring patients with a history of urothelial cell carcinoma (UCC). We determined the frequency of micronucleated urothelial cells (MNC) in exfoliated bladder cells from 105 patients with (n = 52) or without (n = 53) a history of UCC, all of whom tested negative for neoplasia by cytopathological and histopathological analyses. MNC frequencies were increased in patients with a history of UCC (non-smoker and smoker/ex-smoker patients vs non-smoker and smoker/ex-smoker controls; p<0.001), in non-smoker UCC patients (vs non-smoker controls; p<0.01), and in smoker/ex-smoker controls (vs non-smoker controls; p<0.001). Patients with a history of recurrent disease also demonstrated a higher MNC frequency compared to patients with non-recurrent neoplasia. However, logistic regression using smoking habits, age and gender as confounding factors did not confirm MNC frequency as a marker for UCC recurrence. Fluorescent in situ hybridization analysis (using a pan-centromeric probe) showed that micronuclei (MN) arose mainly from clastogenic events regardless of UCC and/or smoking histories. In conclusion, our results confirm previous indications that subjects with a history of UCC harbor genetically unstable cells in the bladder urothelium. Furthermore, these results support using the micronucleus assay as an important tool for monitoring patients with a history of UCC and tumor recurrence.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNESP Univ Estadual Paulista, Fac Med, Botucatu, SP, BrazilUniv Nova Lisboa, Fac Ciencias Med FCM, Ctr Estudos Doencas Cronicas, P-1200 Lisbon, PortugalUNESP Univ Estadual Paulista, Fac Med, Botucatu, SP, BrazilFAPESP: 05/55594-6CNPq: 301079/2009-9Public Library ScienceUniversidade Estadual Paulista (Unesp)Univ Nova LisboaCastro Marcondes, Joao Paulo de [UNESP]Oliveira, Maria Luiza Cotrim Sartor de [UNESP]Gontijo, Alisson M.Camargo, João Lauro Viana de [UNESP]Salvadori, Daisy Maria Favero [UNESP]2014-12-03T13:07:02Z2014-12-03T13:07:02Z2014-01-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://dx.doi.org/10.1371/journal.pone.0086162Plos One. San Francisco: Public Library Science, v. 9, n. 1, 6 p., 2014.1932-6203http://hdl.handle.net/11449/11119110.1371/journal.pone.0086162WOS:000330283100118WOS000330283100118.pdf5051118752980903Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2024-09-03T13:15:05Zoai:repositorio.unesp.br:11449/111191Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:15:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma
title Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma
spellingShingle Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma
Castro Marcondes, Joao Paulo de [UNESP]
title_short Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma
title_full Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma
title_fullStr Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma
title_full_unstemmed Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma
title_sort Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma
author Castro Marcondes, Joao Paulo de [UNESP]
author_facet Castro Marcondes, Joao Paulo de [UNESP]
Oliveira, Maria Luiza Cotrim Sartor de [UNESP]
Gontijo, Alisson M.
Camargo, João Lauro Viana de [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
author_role author
author2 Oliveira, Maria Luiza Cotrim Sartor de [UNESP]
Gontijo, Alisson M.
Camargo, João Lauro Viana de [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Nova Lisboa
dc.contributor.author.fl_str_mv Castro Marcondes, Joao Paulo de [UNESP]
Oliveira, Maria Luiza Cotrim Sartor de [UNESP]
Gontijo, Alisson M.
Camargo, João Lauro Viana de [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
description Bladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify individuals with asymptomatic disease. In this context, we used the micronucleus assay to evaluate whether cytogenetic alterations could be used as biomarkers for monitoring patients with a history of urothelial cell carcinoma (UCC). We determined the frequency of micronucleated urothelial cells (MNC) in exfoliated bladder cells from 105 patients with (n = 52) or without (n = 53) a history of UCC, all of whom tested negative for neoplasia by cytopathological and histopathological analyses. MNC frequencies were increased in patients with a history of UCC (non-smoker and smoker/ex-smoker patients vs non-smoker and smoker/ex-smoker controls; p<0.001), in non-smoker UCC patients (vs non-smoker controls; p<0.01), and in smoker/ex-smoker controls (vs non-smoker controls; p<0.001). Patients with a history of recurrent disease also demonstrated a higher MNC frequency compared to patients with non-recurrent neoplasia. However, logistic regression using smoking habits, age and gender as confounding factors did not confirm MNC frequency as a marker for UCC recurrence. Fluorescent in situ hybridization analysis (using a pan-centromeric probe) showed that micronuclei (MN) arose mainly from clastogenic events regardless of UCC and/or smoking histories. In conclusion, our results confirm previous indications that subjects with a history of UCC harbor genetically unstable cells in the bladder urothelium. Furthermore, these results support using the micronucleus assay as an important tool for monitoring patients with a history of UCC and tumor recurrence.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-03T13:07:02Z
2014-12-03T13:07:02Z
2014-01-22
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0086162
Plos One. San Francisco: Public Library Science, v. 9, n. 1, 6 p., 2014.
1932-6203
http://hdl.handle.net/11449/111191
10.1371/journal.pone.0086162
WOS:000330283100118
WOS000330283100118.pdf
5051118752980903
url http://dx.doi.org/10.1371/journal.pone.0086162
http://hdl.handle.net/11449/111191
identifier_str_mv Plos One. San Francisco: Public Library Science, v. 9, n. 1, 6 p., 2014.
1932-6203
10.1371/journal.pone.0086162
WOS:000330283100118
WOS000330283100118.pdf
5051118752980903
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
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reponame:Repositório Institucional da UNESP
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