Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.33594/000000145 http://hdl.handle.net/11449/190608 |
Resumo: | BACKGROUND/AIMS: Doxorubicin, a chemotherapy drug used successfully for years, could induce cardiotoxicity. Euterpe oleracea Mart. (açai) is a fruit high in antioxidant properties. The aim of this study was to evaluate doxorubicin-induced cardiotoxicity prevention after açai administration. METHODS: A total of 64 male Wistar rats were allocated into 4 groups: control (C), açai (A), doxorubicin (D) and açai-doxorubicin (DA). Rats received regular chow (C and D groups) or chow supplemented with açai 5% (A and DA groups) for 4 weeks. Subsequently, rats received doxorubicin 20 mg/kg (D and DA groups) or saline (C and A groups). Euthanasia was performed 48 hours after doxorubicin injection. Left ventricular function was evaluated by echocardiography in vivo and by isolated heart study ex vivo. Oxidative stress, myocardial metabolism and nitric oxide metabolite were evaluated by spectrophotometry, MMP-2 activity by zymography and caspase-3 and Bcl-2 protein expression by Western blot. RESULTS: Doxorubicin induced decreases in body weight, food and water ingestion. We observed decreases in left ventricular fractional shortening in rats treated with doxorubicin. Additionally, the same rats showed lower +dP/dt and -dP/dt during isolated heart study than those who did not receive doxorubicin. Doxorubicin injection increased caspase-3 protein expression, myocardium lipid hydroperoxide concentration, MMP-2 activity, phosphofructokinase and lactate dehydrogenase activity, and decreased β-hydroxyacyl-CoA dehydrogenase, pyruvate dehydrogenase, citrate synthase, complex I, complex II and ATP synthase activity in myocardium. Açai supplementation improved left ventricular fractional shortening, decreased myocardium lipid hydroperoxide concentration, MMP-2 activity, and improved β-hydroxyacyl-CoA dehydrogenase, phosphofructokinase, citrate synthase, complex II and ATP synthase enzymatic activities. We did not observe differences in nitric oxide metabolite concentrations between groups. CONCLUSION: Doxorubicin induced left ventricular dysfunction, increases in oxidative stress, changes in myocardium metabolism and MMP-2 activation. Açai supplementation was able to prevent these alterations. |
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Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in RatsApoptosisCardiac functionMatrix metalloproteinase-2Oxidative stressBACKGROUND/AIMS: Doxorubicin, a chemotherapy drug used successfully for years, could induce cardiotoxicity. Euterpe oleracea Mart. (açai) is a fruit high in antioxidant properties. The aim of this study was to evaluate doxorubicin-induced cardiotoxicity prevention after açai administration. METHODS: A total of 64 male Wistar rats were allocated into 4 groups: control (C), açai (A), doxorubicin (D) and açai-doxorubicin (DA). Rats received regular chow (C and D groups) or chow supplemented with açai 5% (A and DA groups) for 4 weeks. Subsequently, rats received doxorubicin 20 mg/kg (D and DA groups) or saline (C and A groups). Euthanasia was performed 48 hours after doxorubicin injection. Left ventricular function was evaluated by echocardiography in vivo and by isolated heart study ex vivo. Oxidative stress, myocardial metabolism and nitric oxide metabolite were evaluated by spectrophotometry, MMP-2 activity by zymography and caspase-3 and Bcl-2 protein expression by Western blot. RESULTS: Doxorubicin induced decreases in body weight, food and water ingestion. We observed decreases in left ventricular fractional shortening in rats treated with doxorubicin. Additionally, the same rats showed lower +dP/dt and -dP/dt during isolated heart study than those who did not receive doxorubicin. Doxorubicin injection increased caspase-3 protein expression, myocardium lipid hydroperoxide concentration, MMP-2 activity, phosphofructokinase and lactate dehydrogenase activity, and decreased β-hydroxyacyl-CoA dehydrogenase, pyruvate dehydrogenase, citrate synthase, complex I, complex II and ATP synthase activity in myocardium. Açai supplementation improved left ventricular fractional shortening, decreased myocardium lipid hydroperoxide concentration, MMP-2 activity, and improved β-hydroxyacyl-CoA dehydrogenase, phosphofructokinase, citrate synthase, complex II and ATP synthase enzymatic activities. We did not observe differences in nitric oxide metabolite concentrations between groups. CONCLUSION: Doxorubicin induced left ventricular dysfunction, increases in oxidative stress, changes in myocardium metabolism and MMP-2 activation. Açai supplementation was able to prevent these alterations.Debreceni EgyetemMedical School São Paulo State University (Unesp)Institute of Biosciences São Paulo State University (Unesp)Medical School São Paulo State University (Unesp)Institute of Biosciences São Paulo State University (Unesp)Debreceni Egyetem: 2018/03381-9Universidade Estadual Paulista (Unesp)Mathias, Lívia Maria Beraldo Simões [UNESP]Alegre, Patricia Helena Correa [UNESP]Dos Santos, Isadora de Oliveira Fernandes [UNESP]Bachiega, Tatiana [UNESP]Figueiredo, Amanda Menezes [UNESP]Chiuso-Minicucci, Fernanda [UNESP]Fernandes, Ana Angélica [UNESP]Bazan, Silméia Garcia Zanatti [UNESP]Minicucci, Marcos Ferreira [UNESP]Azevedo, Paula Schmidt [UNESP]Okoshi, Marina Politi [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]Paiva, Sergio Alberto Rupp [UNESP]Polegato, Bertha Furlan [UNESP]2019-10-06T17:18:55Z2019-10-06T17:18:55Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article388-399http://dx.doi.org/10.33594/000000145Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, v. 53, n. 2, p. 388-399, 2019.1421-9778http://hdl.handle.net/11449/19060810.33594/0000001452-s2.0-8507135552850168390153945471213140801402647743870403447167344631386719984320000-0002-5843-6232Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyinfo:eu-repo/semantics/openAccess2021-10-23T19:49:53Zoai:repositorio.unesp.br:11449/190608Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:49:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats |
title |
Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats |
spellingShingle |
Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats Mathias, Lívia Maria Beraldo Simões [UNESP] Apoptosis Cardiac function Matrix metalloproteinase-2 Oxidative stress |
title_short |
Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats |
title_full |
Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats |
title_fullStr |
Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats |
title_full_unstemmed |
Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats |
title_sort |
Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats |
author |
Mathias, Lívia Maria Beraldo Simões [UNESP] |
author_facet |
Mathias, Lívia Maria Beraldo Simões [UNESP] Alegre, Patricia Helena Correa [UNESP] Dos Santos, Isadora de Oliveira Fernandes [UNESP] Bachiega, Tatiana [UNESP] Figueiredo, Amanda Menezes [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Fernandes, Ana Angélica [UNESP] Bazan, Silméia Garcia Zanatti [UNESP] Minicucci, Marcos Ferreira [UNESP] Azevedo, Paula Schmidt [UNESP] Okoshi, Marina Politi [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Paiva, Sergio Alberto Rupp [UNESP] Polegato, Bertha Furlan [UNESP] |
author_role |
author |
author2 |
Alegre, Patricia Helena Correa [UNESP] Dos Santos, Isadora de Oliveira Fernandes [UNESP] Bachiega, Tatiana [UNESP] Figueiredo, Amanda Menezes [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Fernandes, Ana Angélica [UNESP] Bazan, Silméia Garcia Zanatti [UNESP] Minicucci, Marcos Ferreira [UNESP] Azevedo, Paula Schmidt [UNESP] Okoshi, Marina Politi [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Paiva, Sergio Alberto Rupp [UNESP] Polegato, Bertha Furlan [UNESP] |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mathias, Lívia Maria Beraldo Simões [UNESP] Alegre, Patricia Helena Correa [UNESP] Dos Santos, Isadora de Oliveira Fernandes [UNESP] Bachiega, Tatiana [UNESP] Figueiredo, Amanda Menezes [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Fernandes, Ana Angélica [UNESP] Bazan, Silméia Garcia Zanatti [UNESP] Minicucci, Marcos Ferreira [UNESP] Azevedo, Paula Schmidt [UNESP] Okoshi, Marina Politi [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Paiva, Sergio Alberto Rupp [UNESP] Polegato, Bertha Furlan [UNESP] |
dc.subject.por.fl_str_mv |
Apoptosis Cardiac function Matrix metalloproteinase-2 Oxidative stress |
topic |
Apoptosis Cardiac function Matrix metalloproteinase-2 Oxidative stress |
description |
BACKGROUND/AIMS: Doxorubicin, a chemotherapy drug used successfully for years, could induce cardiotoxicity. Euterpe oleracea Mart. (açai) is a fruit high in antioxidant properties. The aim of this study was to evaluate doxorubicin-induced cardiotoxicity prevention after açai administration. METHODS: A total of 64 male Wistar rats were allocated into 4 groups: control (C), açai (A), doxorubicin (D) and açai-doxorubicin (DA). Rats received regular chow (C and D groups) or chow supplemented with açai 5% (A and DA groups) for 4 weeks. Subsequently, rats received doxorubicin 20 mg/kg (D and DA groups) or saline (C and A groups). Euthanasia was performed 48 hours after doxorubicin injection. Left ventricular function was evaluated by echocardiography in vivo and by isolated heart study ex vivo. Oxidative stress, myocardial metabolism and nitric oxide metabolite were evaluated by spectrophotometry, MMP-2 activity by zymography and caspase-3 and Bcl-2 protein expression by Western blot. RESULTS: Doxorubicin induced decreases in body weight, food and water ingestion. We observed decreases in left ventricular fractional shortening in rats treated with doxorubicin. Additionally, the same rats showed lower +dP/dt and -dP/dt during isolated heart study than those who did not receive doxorubicin. Doxorubicin injection increased caspase-3 protein expression, myocardium lipid hydroperoxide concentration, MMP-2 activity, phosphofructokinase and lactate dehydrogenase activity, and decreased β-hydroxyacyl-CoA dehydrogenase, pyruvate dehydrogenase, citrate synthase, complex I, complex II and ATP synthase activity in myocardium. Açai supplementation improved left ventricular fractional shortening, decreased myocardium lipid hydroperoxide concentration, MMP-2 activity, and improved β-hydroxyacyl-CoA dehydrogenase, phosphofructokinase, citrate synthase, complex II and ATP synthase enzymatic activities. We did not observe differences in nitric oxide metabolite concentrations between groups. CONCLUSION: Doxorubicin induced left ventricular dysfunction, increases in oxidative stress, changes in myocardium metabolism and MMP-2 activation. Açai supplementation was able to prevent these alterations. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:18:55Z 2019-10-06T17:18:55Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.33594/000000145 Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, v. 53, n. 2, p. 388-399, 2019. 1421-9778 http://hdl.handle.net/11449/190608 10.33594/000000145 2-s2.0-85071355528 5016839015394547 1213140801402647 7438704034471673 4463138671998432 0000-0002-5843-6232 |
url |
http://dx.doi.org/10.33594/000000145 http://hdl.handle.net/11449/190608 |
identifier_str_mv |
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, v. 53, n. 2, p. 388-399, 2019. 1421-9778 10.33594/000000145 2-s2.0-85071355528 5016839015394547 1213140801402647 7438704034471673 4463138671998432 0000-0002-5843-6232 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
388-399 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964942342815744 |