Desenvolvimento, caracterização e comparação de formulações para aplicação tópica contendo curcumina: estudos de efetividade e segurança das formulações

• Autor(a) principal: Fernandes, Lígia de Souza [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/151621
• topic: Antifungico; Análise estrutural; Candida albicans; Curcumina; Ensaio anti-CVV in vivo; Estabilidade; Liberação in vitro; Validação de método analítico; Antifungal; Structural analysis; Curcumin; Anti-CVV in vivo assay; Stability; In vitro release; Validation of analytical method

Curcumin is the main active compound found in rhizome of Curcuma Longa L. plant and has several pharmacological activities. Several studies have shown that curcumin has excellent antifungal activity and it is a promising natural active compound for treatment of fungal diseases. The research objective was formulations development containing curcumin that may have action against vulvovaginal candidiasis (VVC). For formulations structural analysis were used techniques as polarized light microscopy, small angle X-ray scattering, X-ray diffraction and rheological behavior, and the developed systems has presented adequate and satisfactory characteristics according with research objective and with mucoadhesive properties. The proposed analytical method for curcumin quantification was revalidated, then used in stability study and in vitro release assays. The formulations were submitted to a preliminary stability study to guarantee its safety and efficacy, this way stability was proven. Subsequently were performed in vitro release tests to verify the curcumin ability of release from formulations and to exert its pharmacological activity. The emulsified and pseudoternary systems presented release rates of 0.48% and 0.78% in 12 hours. With the in vitro pharmacological activity test was determined the minimal inhibitory concentration against yeast Candida albicans and also the effective concentration to be incorporated in the systems. After these tests was performed in vivo anti-VVC activity, the formulations has presented satisfactory and significant results regarding reduction of the fungal load in infected animals. Thus curcumin incorporated in the developed systems can be considered as a new possibility in antifungal therapy.