Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/15287394.2018.1437581 http://hdl.handle.net/11449/179580 |
Resumo: | Imidacloprid (IMD) is a neonicotinoid insecticide widely used in crops, pets, and on farm animals for pest control. Several studies were conducted examining the adverse effects of IMD on animals often exhibiting hepatic damage. The aim of this study was to determine the effects of IMD on bioenergetics of mitochondria isolated from rat liver. Imidacloprid (50–200 µM) produced a concentration-dependent decrease in oxygen consumption and ATP production without markedly affecting mitochondrial membrane potential (MMP). Oxygen consumption experiments showed that IMD did not significantly affect the respiratory chain, and this was similar to findings with oligomycin and carboxyatractyloside, suggesting a direct action on FoF1-ATP synthase and/or the adenine nucleotide translocator (ANT). Imidacloprid inhibited FoF1-ATP synthase activity only in disrupted mitochondria and induced a partial inhibition of ADP-stimulated depolarization of the MMP. Our results indicate that IMD interacts specifically with FoF1-ATP synthase resulting in functional inhibition of the enzyme with consequent impairment of mitochondrial bioenergetics. These effects of IMD on mitochondrial bioenergetics may be related to adverse effects of this insecticide on the liver. |
id |
UNSP_71d666616b4dc6b521d164df8e53b17a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/179580 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activityImidacloprid (IMD) is a neonicotinoid insecticide widely used in crops, pets, and on farm animals for pest control. Several studies were conducted examining the adverse effects of IMD on animals often exhibiting hepatic damage. The aim of this study was to determine the effects of IMD on bioenergetics of mitochondria isolated from rat liver. Imidacloprid (50–200 µM) produced a concentration-dependent decrease in oxygen consumption and ATP production without markedly affecting mitochondrial membrane potential (MMP). Oxygen consumption experiments showed that IMD did not significantly affect the respiratory chain, and this was similar to findings with oligomycin and carboxyatractyloside, suggesting a direct action on FoF1-ATP synthase and/or the adenine nucleotide translocator (ANT). Imidacloprid inhibited FoF1-ATP synthase activity only in disrupted mitochondria and induced a partial inhibition of ADP-stimulated depolarization of the MMP. Our results indicate that IMD interacts specifically with FoF1-ATP synthase resulting in functional inhibition of the enzyme with consequent impairment of mitochondrial bioenergetics. These effects of IMD on mitochondrial bioenergetics may be related to adverse effects of this insecticide on the liver.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)College of Agricultural and Technological Sciences São Paulo State University (Unesp)School of Veterinary Medicine São Paulo State University (Unesp)College of Agricultural and Technological Sciences São Paulo State University (Unesp)School of Veterinary Medicine São Paulo State University (Unesp)FAPESP: 2015/19549-8Universidade Estadual Paulista (Unesp)Bizerra, Paulo F. V. [UNESP]Guimarães, Anilda R. J. S. [UNESP]Maioli, Marcos A. [UNESP]Mingatto, Fábio E. [UNESP]2018-12-11T17:35:53Z2018-12-11T17:35:53Z2018-04-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article229-239application/pdfhttp://dx.doi.org/10.1080/15287394.2018.1437581Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 81, n. 8, p. 229-239, 2018.1087-26201528-7394http://hdl.handle.net/11449/17958010.1080/15287394.2018.14375812-s2.0-850419121122-s2.0-85041912112.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Toxicology and Environmental Health - Part A: Current Issues0,8880,888info:eu-repo/semantics/openAccess2024-05-07T13:47:22Zoai:repositorio.unesp.br:11449/179580Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:44:44.644481Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity |
title |
Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity |
spellingShingle |
Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity Bizerra, Paulo F. V. [UNESP] |
title_short |
Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity |
title_full |
Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity |
title_fullStr |
Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity |
title_full_unstemmed |
Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity |
title_sort |
Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity |
author |
Bizerra, Paulo F. V. [UNESP] |
author_facet |
Bizerra, Paulo F. V. [UNESP] Guimarães, Anilda R. J. S. [UNESP] Maioli, Marcos A. [UNESP] Mingatto, Fábio E. [UNESP] |
author_role |
author |
author2 |
Guimarães, Anilda R. J. S. [UNESP] Maioli, Marcos A. [UNESP] Mingatto, Fábio E. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bizerra, Paulo F. V. [UNESP] Guimarães, Anilda R. J. S. [UNESP] Maioli, Marcos A. [UNESP] Mingatto, Fábio E. [UNESP] |
description |
Imidacloprid (IMD) is a neonicotinoid insecticide widely used in crops, pets, and on farm animals for pest control. Several studies were conducted examining the adverse effects of IMD on animals often exhibiting hepatic damage. The aim of this study was to determine the effects of IMD on bioenergetics of mitochondria isolated from rat liver. Imidacloprid (50–200 µM) produced a concentration-dependent decrease in oxygen consumption and ATP production without markedly affecting mitochondrial membrane potential (MMP). Oxygen consumption experiments showed that IMD did not significantly affect the respiratory chain, and this was similar to findings with oligomycin and carboxyatractyloside, suggesting a direct action on FoF1-ATP synthase and/or the adenine nucleotide translocator (ANT). Imidacloprid inhibited FoF1-ATP synthase activity only in disrupted mitochondria and induced a partial inhibition of ADP-stimulated depolarization of the MMP. Our results indicate that IMD interacts specifically with FoF1-ATP synthase resulting in functional inhibition of the enzyme with consequent impairment of mitochondrial bioenergetics. These effects of IMD on mitochondrial bioenergetics may be related to adverse effects of this insecticide on the liver. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:35:53Z 2018-12-11T17:35:53Z 2018-04-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/15287394.2018.1437581 Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 81, n. 8, p. 229-239, 2018. 1087-2620 1528-7394 http://hdl.handle.net/11449/179580 10.1080/15287394.2018.1437581 2-s2.0-85041912112 2-s2.0-85041912112.pdf |
url |
http://dx.doi.org/10.1080/15287394.2018.1437581 http://hdl.handle.net/11449/179580 |
identifier_str_mv |
Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 81, n. 8, p. 229-239, 2018. 1087-2620 1528-7394 10.1080/15287394.2018.1437581 2-s2.0-85041912112 2-s2.0-85041912112.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Toxicology and Environmental Health - Part A: Current Issues 0,888 0,888 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
229-239 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128694678454272 |