Systemic cytokine profiles of CD4+ t lymphocytes correlate with clinical features and functional status in stable COPD

• Autor(a) principal: Uzeloto, Juliana Souza [UNESP]
• Data de Publicação: 2020
• Outros Autores: de Toledo-Arruda, Alessandra Choqueta, Silva, Bruna Spolador de Alencar [UNESP], Golim, Marjorie de Assis [UNESP], Braz, Aline Márcia Marques [UNESP], Grigoletto, Fabiano Francisco de Lima Isis [UNESP], Ramos, Ercy Mara Cipulo [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.2147/COPD.S268955; http://hdl.handle.net/11449/208141
• topic: Chronic obstructive pulmonary disease; Flow cytometry; Inflammation; Phenotype

Aims: To evaluate the expressions of intracellular cytokines in CD4+ T lymphocytes and to investigate the correlation between biomarker expressions and clinical and functional characteristics of stable COPD patients. Patients and Methods: Peripheral blood was collected from 36 COPD patients, and the expression of cytokines (IL-8, IL-13, IL-17, IL-6, IL-2, IL-10, and TNF-α) in T lymphocytes CD4 + was investigated. In addition, lung function, dyspnea symptoms, quality of life, vital signs, body composition, level of physical activity, peripheral muscle strength, and functional capacity were assessed. Results: Individuals with greater bronchial obstruction present a higher proportion of CD4 + IL-2 + lymphocytes compared to individuals with less severe bronchial obstruction. We found a positive correlation between the expression of the cytokines IL-13, IL-17, IL-6, IL-2, IL-10, and TNF-α in CD4+ T lymphocytes. In addition, we found a positive correlation between CD4+ IL-10+ T lymphocytes and lower limb muscle strength and a negative correlation between CD4+ IL-8+ T lymphocytes and peripheral oxygen saturation and steps per day. Conclusion: Systemic CD4+IL-2+, IL-8+, and IL-10+ T lymphocytes presented a correlation with clinical characteristics and functional status in stable COPD.