Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3844/ojbsci.2015.134.142 http://hdl.handle.net/11449/172024 |
Resumo: | Alcohol exerts teratogenic effects and its consumption during pregnancy may cause various alterations in the fetus, including deficit of bone development. The objective of this study was to evaluate the initial responses, on osteoblasts isolated from newborn rat calvaria, after prenatal ethanol exposure. Nine pregnant rats were divided into three groups according to the diet fed during pregnancy: Rats fed 20% ethanol, Pair-fed and control were the groups. At 3 days of life, newborn rats were euthanized for removal of the calvaria and isolation of osteogenic cells by sequential enzymatic digestion. The cells were cultured for a maximum period of 14 days. The effect of alcohol was investigated by the measurement of cell adhesion, proliferation and viability, total protein content, Alkaline Phosphatase (ALP) activity and bone matrix formation. The results showed the highest proliferation in ETH group on the 3<sup>th</sup> day and the highest ALP activity and bone matrix formation, in this group, on the 14<sup>th</sup> day, indicating that prenatal ethanol seems to affect the proliferation earlyand the ALP activity and bone matrix formation in more advanced periods. |
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Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn ratsCell cultureEthanolGestationOsteoblastsRatAlcohol exerts teratogenic effects and its consumption during pregnancy may cause various alterations in the fetus, including deficit of bone development. The objective of this study was to evaluate the initial responses, on osteoblasts isolated from newborn rat calvaria, after prenatal ethanol exposure. Nine pregnant rats were divided into three groups according to the diet fed during pregnancy: Rats fed 20% ethanol, Pair-fed and control were the groups. At 3 days of life, newborn rats were euthanized for removal of the calvaria and isolation of osteogenic cells by sequential enzymatic digestion. The cells were cultured for a maximum period of 14 days. The effect of alcohol was investigated by the measurement of cell adhesion, proliferation and viability, total protein content, Alkaline Phosphatase (ALP) activity and bone matrix formation. The results showed the highest proliferation in ETH group on the 3<sup>th</sup> day and the highest ALP activity and bone matrix formation, in this group, on the 14<sup>th</sup> day, indicating that prenatal ethanol seems to affect the proliferation earlyand the ALP activity and bone matrix formation in more advanced periods.Institute of Science and Technology, UNESP-Univ Estadual Paulista, Sao Jose dos Campos (SP), School of Dentistry, Department of Biosciences and Oral DiagnosisInstitute of Research and Development-IPandD, Universidade do Vale do Paraíba-UNIVAP, Laboratory Dynamics of Cellular CompartmentsInstitute of Science and Technology, UNESP-Univ Estadual Paulista, Sao Jose dos Campos (SP), School of Dentistry, Department of Biosciences and Oral DiagnosisUniversidade Estadual Paulista (Unesp)Institute of Research and Development-IPandD, Universidade do Vale do Paraíba-UNIVAP, Laboratory Dynamics of Cellular CompartmentsCarvalho, Isabel Chaves Silva [UNESP]De Andrade, Dennia Perez [UNESP]Milhan, Noala Vicensoto Moreira [UNESP]De Souza Santos, Evelyn Luzia [UNESP]Soares, Cristina PachecoDa Rocha, Rosilene Fernandes [UNESP]De Vasconcellos, Luana Marotta Reis [UNESP]2018-12-11T16:58:11Z2018-12-11T16:58:11Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article134-142application/pdfhttp://dx.doi.org/10.3844/ojbsci.2015.134.142OnLine Journal of Biological Sciences, v. 15, n. 3, p. 134-142, 2015.1608-4217http://hdl.handle.net/11449/17202410.3844/ojbsci.2015.134.1422-s2.0-849401764192-s2.0-84940176419.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOnLine Journal of Biological Sciences0,166info:eu-repo/semantics/openAccess2023-12-02T06:16:41Zoai:repositorio.unesp.br:11449/172024Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-02T06:16:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats |
title |
Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats |
spellingShingle |
Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats Carvalho, Isabel Chaves Silva [UNESP] Cell culture Ethanol Gestation Osteoblasts Rat |
title_short |
Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats |
title_full |
Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats |
title_fullStr |
Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats |
title_full_unstemmed |
Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats |
title_sort |
Prenatal ethanol exposure affects the proliferation and differentiation of the osteoblasts from newborn rats |
author |
Carvalho, Isabel Chaves Silva [UNESP] |
author_facet |
Carvalho, Isabel Chaves Silva [UNESP] De Andrade, Dennia Perez [UNESP] Milhan, Noala Vicensoto Moreira [UNESP] De Souza Santos, Evelyn Luzia [UNESP] Soares, Cristina Pacheco Da Rocha, Rosilene Fernandes [UNESP] De Vasconcellos, Luana Marotta Reis [UNESP] |
author_role |
author |
author2 |
De Andrade, Dennia Perez [UNESP] Milhan, Noala Vicensoto Moreira [UNESP] De Souza Santos, Evelyn Luzia [UNESP] Soares, Cristina Pacheco Da Rocha, Rosilene Fernandes [UNESP] De Vasconcellos, Luana Marotta Reis [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Institute of Research and Development-IPandD, Universidade do Vale do Paraíba-UNIVAP, Laboratory Dynamics of Cellular Compartments |
dc.contributor.author.fl_str_mv |
Carvalho, Isabel Chaves Silva [UNESP] De Andrade, Dennia Perez [UNESP] Milhan, Noala Vicensoto Moreira [UNESP] De Souza Santos, Evelyn Luzia [UNESP] Soares, Cristina Pacheco Da Rocha, Rosilene Fernandes [UNESP] De Vasconcellos, Luana Marotta Reis [UNESP] |
dc.subject.por.fl_str_mv |
Cell culture Ethanol Gestation Osteoblasts Rat |
topic |
Cell culture Ethanol Gestation Osteoblasts Rat |
description |
Alcohol exerts teratogenic effects and its consumption during pregnancy may cause various alterations in the fetus, including deficit of bone development. The objective of this study was to evaluate the initial responses, on osteoblasts isolated from newborn rat calvaria, after prenatal ethanol exposure. Nine pregnant rats were divided into three groups according to the diet fed during pregnancy: Rats fed 20% ethanol, Pair-fed and control were the groups. At 3 days of life, newborn rats were euthanized for removal of the calvaria and isolation of osteogenic cells by sequential enzymatic digestion. The cells were cultured for a maximum period of 14 days. The effect of alcohol was investigated by the measurement of cell adhesion, proliferation and viability, total protein content, Alkaline Phosphatase (ALP) activity and bone matrix formation. The results showed the highest proliferation in ETH group on the 3<sup>th</sup> day and the highest ALP activity and bone matrix formation, in this group, on the 14<sup>th</sup> day, indicating that prenatal ethanol seems to affect the proliferation earlyand the ALP activity and bone matrix formation in more advanced periods. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-12-11T16:58:11Z 2018-12-11T16:58:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3844/ojbsci.2015.134.142 OnLine Journal of Biological Sciences, v. 15, n. 3, p. 134-142, 2015. 1608-4217 http://hdl.handle.net/11449/172024 10.3844/ojbsci.2015.134.142 2-s2.0-84940176419 2-s2.0-84940176419.pdf |
url |
http://dx.doi.org/10.3844/ojbsci.2015.134.142 http://hdl.handle.net/11449/172024 |
identifier_str_mv |
OnLine Journal of Biological Sciences, v. 15, n. 3, p. 134-142, 2015. 1608-4217 10.3844/ojbsci.2015.134.142 2-s2.0-84940176419 2-s2.0-84940176419.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
OnLine Journal of Biological Sciences 0,166 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
134-142 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965148023095296 |