Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats

Detalhes bibliográficos
Autor(a) principal: Cesar-Tognoli, Lilian M. M. [UNESP]
Data de Publicação: 2011
Outros Autores: Salamoni, Simone D., Tavares, Andrea A., Elias, Carol F., Da Costa, Jaderson C., Bittencourt, Jackson C., Palma, Mario Sergio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/toxins3020142
http://hdl.handle.net/11449/20015
Resumo: The 6-hydroxytrypargine (6-HT) is an alkaloidal toxin of the group of tetrahydro-beta-carbolines (TH beta C) isolated from the venom of the colonial spider Parawixia bistriata. These alkaloids are reversible inhibitors of the monoamine-oxidase enzyme (MAO), with hallucinogenic, tremorigenic and anxiolytic properties. The toxin 6-HT was the first TH beta C chemically reported in the venom of spiders; however, it was not functionally well characterized up to now. The action of 6-HT was investigated by intracerebroventricular (i.c.v.) and intravenous (i.v.) applications of the toxin in adult male Wistar rats, followed by the monitoring of the expression of fos-protein, combined with the use of double labeling immunehistochemistry protocols for the detection of some nervous receptors and enzymes related to the metabolism of neurotransmitters in the central nervous system (CNS). We also investigated the epileptiform activity in presence of this toxin. The assays were carried out in normal hippocampal neurons and also in a model of chronic epilepsy obtained by the use of neurons incubated in free-magnesium artificial cerebro-spinal fluid (ACSF). Trypargine, a well known TH beta C toxin, was used as standard compound for comparative purposes. Fos-immunoreactive cells (fos-ir) were observed in hypothalamic and thalamic areas, while the double-labeling identified nervous receptors of the sub-types rGlu2/3 and NMR1, and orexinergic neurons. The 6-HT was administrated by perfusion and ejection in brain slices of hippocampus, inducing epileptic activity after its administration; the toxin was not able to block the epileptogenic crisis observed in the chronic model of the epilepsy, suggesting that 6-HT did not block the overactive GluRs responsible for this epileptic activity.
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spelling Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats6-hydroxytryparginefos proteinimmunohistochemistryhypothalamushippocampusepilepsyNMDAneurotoxicityThe 6-hydroxytrypargine (6-HT) is an alkaloidal toxin of the group of tetrahydro-beta-carbolines (TH beta C) isolated from the venom of the colonial spider Parawixia bistriata. These alkaloids are reversible inhibitors of the monoamine-oxidase enzyme (MAO), with hallucinogenic, tremorigenic and anxiolytic properties. The toxin 6-HT was the first TH beta C chemically reported in the venom of spiders; however, it was not functionally well characterized up to now. The action of 6-HT was investigated by intracerebroventricular (i.c.v.) and intravenous (i.v.) applications of the toxin in adult male Wistar rats, followed by the monitoring of the expression of fos-protein, combined with the use of double labeling immunehistochemistry protocols for the detection of some nervous receptors and enzymes related to the metabolism of neurotransmitters in the central nervous system (CNS). We also investigated the epileptiform activity in presence of this toxin. The assays were carried out in normal hippocampal neurons and also in a model of chronic epilepsy obtained by the use of neurons incubated in free-magnesium artificial cerebro-spinal fluid (ACSF). Trypargine, a well known TH beta C toxin, was used as standard compound for comparative purposes. Fos-immunoreactive cells (fos-ir) were observed in hypothalamic and thalamic areas, while the double-labeling identified nervous receptors of the sub-types rGlu2/3 and NMR1, and orexinergic neurons. The 6-HT was administrated by perfusion and ejection in brain slices of hippocampus, inducing epileptic activity after its administration; the toxin was not able to block the epileptogenic crisis observed in the chronic model of the epilepsy, suggesting that 6-HT did not block the overactive GluRs responsible for this epileptic activity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State Univ UNESP, Inst Biosci, CEIS, Dept Biol,Lab Struct Biol & Zoochem, BR-13506900 Rio Claro, SP, BrazilPontif Catholic Univ Rio Grande Sul PUCRS, Inst Biomed Res, Neurosci Lab, BR-90619900 Porto Alegre, RS, BrazilUniv São Paulo, Inst Biomed Sci, Lab Chem Neuroanat, Dept Anat, BR-05508900 São Paulo, BrazilPontif Catholic Univ Rio Grande Sul PUCRS, Brain Inst InsCer, BR-90619900 Porto Alegre, RS, BrazilSão Paulo State Univ UNESP, Inst Biosci, CEIS, Dept Biol,Lab Struct Biol & Zoochem, BR-13506900 Rio Claro, SP, BrazilFAPESP: 04/07942-2FAPESP: 06/57122-7Mdpi AgUniversidade Estadual Paulista (Unesp)Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)Universidade de São Paulo (USP)Cesar-Tognoli, Lilian M. M. [UNESP]Salamoni, Simone D.Tavares, Andrea A.Elias, Carol F.Da Costa, Jaderson C.Bittencourt, Jackson C.Palma, Mario Sergio [UNESP]2014-05-20T13:55:55Z2014-05-20T13:55:55Z2011-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article142-162application/pdfhttp://dx.doi.org/10.3390/toxins3020142Toxins. Basel: Mdpi Ag, v. 3, n. 2, p. 142-162, 2011.2072-6651http://hdl.handle.net/11449/2001510.3390/toxins3020142WOS:000307974700003WOS000307974700003.pdf2901888624506535Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxins3.2730,955info:eu-repo/semantics/openAccess2023-10-22T06:03:39Zoai:repositorio.unesp.br:11449/20015Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:34:16.334020Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats
title Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats
spellingShingle Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats
Cesar-Tognoli, Lilian M. M. [UNESP]
6-hydroxytrypargine
fos protein
immunohistochemistry
hypothalamus
hippocampus
epilepsy
NMDA
neurotoxicity
title_short Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats
title_full Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats
title_fullStr Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats
title_full_unstemmed Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats
title_sort Effects of Spider Venom Toxin PWTX-I (6-Hydroxytrypargine) on the Central Nervous System of Rats
author Cesar-Tognoli, Lilian M. M. [UNESP]
author_facet Cesar-Tognoli, Lilian M. M. [UNESP]
Salamoni, Simone D.
Tavares, Andrea A.
Elias, Carol F.
Da Costa, Jaderson C.
Bittencourt, Jackson C.
Palma, Mario Sergio [UNESP]
author_role author
author2 Salamoni, Simone D.
Tavares, Andrea A.
Elias, Carol F.
Da Costa, Jaderson C.
Bittencourt, Jackson C.
Palma, Mario Sergio [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Cesar-Tognoli, Lilian M. M. [UNESP]
Salamoni, Simone D.
Tavares, Andrea A.
Elias, Carol F.
Da Costa, Jaderson C.
Bittencourt, Jackson C.
Palma, Mario Sergio [UNESP]
dc.subject.por.fl_str_mv 6-hydroxytrypargine
fos protein
immunohistochemistry
hypothalamus
hippocampus
epilepsy
NMDA
neurotoxicity
topic 6-hydroxytrypargine
fos protein
immunohistochemistry
hypothalamus
hippocampus
epilepsy
NMDA
neurotoxicity
description The 6-hydroxytrypargine (6-HT) is an alkaloidal toxin of the group of tetrahydro-beta-carbolines (TH beta C) isolated from the venom of the colonial spider Parawixia bistriata. These alkaloids are reversible inhibitors of the monoamine-oxidase enzyme (MAO), with hallucinogenic, tremorigenic and anxiolytic properties. The toxin 6-HT was the first TH beta C chemically reported in the venom of spiders; however, it was not functionally well characterized up to now. The action of 6-HT was investigated by intracerebroventricular (i.c.v.) and intravenous (i.v.) applications of the toxin in adult male Wistar rats, followed by the monitoring of the expression of fos-protein, combined with the use of double labeling immunehistochemistry protocols for the detection of some nervous receptors and enzymes related to the metabolism of neurotransmitters in the central nervous system (CNS). We also investigated the epileptiform activity in presence of this toxin. The assays were carried out in normal hippocampal neurons and also in a model of chronic epilepsy obtained by the use of neurons incubated in free-magnesium artificial cerebro-spinal fluid (ACSF). Trypargine, a well known TH beta C toxin, was used as standard compound for comparative purposes. Fos-immunoreactive cells (fos-ir) were observed in hypothalamic and thalamic areas, while the double-labeling identified nervous receptors of the sub-types rGlu2/3 and NMR1, and orexinergic neurons. The 6-HT was administrated by perfusion and ejection in brain slices of hippocampus, inducing epileptic activity after its administration; the toxin was not able to block the epileptogenic crisis observed in the chronic model of the epilepsy, suggesting that 6-HT did not block the overactive GluRs responsible for this epileptic activity.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
2014-05-20T13:55:55Z
2014-05-20T13:55:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/toxins3020142
Toxins. Basel: Mdpi Ag, v. 3, n. 2, p. 142-162, 2011.
2072-6651
http://hdl.handle.net/11449/20015
10.3390/toxins3020142
WOS:000307974700003
WOS000307974700003.pdf
2901888624506535
url http://dx.doi.org/10.3390/toxins3020142
http://hdl.handle.net/11449/20015
identifier_str_mv Toxins. Basel: Mdpi Ag, v. 3, n. 2, p. 142-162, 2011.
2072-6651
10.3390/toxins3020142
WOS:000307974700003
WOS000307974700003.pdf
2901888624506535
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxins
3.273
0,955
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dc.format.none.fl_str_mv 142-162
application/pdf
dc.publisher.none.fl_str_mv Mdpi Ag
publisher.none.fl_str_mv Mdpi Ag
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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