Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cropro.2019.104871 http://hdl.handle.net/11449/190462 |
Resumo: | Spodoptera frugiperda (J.E. Smith) is the main pest of maize in Brazil and its control has been targeted by Bt maize hybrids. Generally, Bt maize hybrids possess the transgenic locus in a hemizygous condition, that is, containing only one copy of the Bt allele. However, studies have shown that maize hybrids with an additional transgenic allele, namely, in a homozygous state, increases transgenic protein expression. Our aim in this study was to evaluate whether the transgenic event TC1507 x MON89034 x NK603 in homozygosis increases Bt protein expression levels and, consequently, reduces S. frugiperda leaf-feeding injury and larval survival which affects maize grain yield. Leaf-feeding injury of S. frugiperda was 29% lower on homozygous hybrids relative to their isogenic hemizygous versions. Isogenic homozygous and hemizygous hybrids did not differ in grain yield in this study. S. frugiperda survivorship on homozygous hybrids was significantly lower than on their hemizygous isogenic versions (16.9% and 38.5%, respectively). Homozygous hybrids presented higher Cry1F, Cry1A.105, and Cry2Ab2 protein expression levels relative to their isogenic hemizygous versions (approximately 1.5-, 2.0-, and 2.5-fold, respectively). The Bt maize event TC1507 x MON89034 x NK603 in a homozygous state increases Bt protein expression levels and the control of S. frugiperda. Therefore, the deployment of homozygous transgenic maize hybrids to farmers is more desirable than the hemizygous versions. |
id |
UNSP_72a296837f93081a0c38f8b4cdb8c961 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/190462 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybridsBt protein concentrationFall armywormResistance managementZygositySpodoptera frugiperda (J.E. Smith) is the main pest of maize in Brazil and its control has been targeted by Bt maize hybrids. Generally, Bt maize hybrids possess the transgenic locus in a hemizygous condition, that is, containing only one copy of the Bt allele. However, studies have shown that maize hybrids with an additional transgenic allele, namely, in a homozygous state, increases transgenic protein expression. Our aim in this study was to evaluate whether the transgenic event TC1507 x MON89034 x NK603 in homozygosis increases Bt protein expression levels and, consequently, reduces S. frugiperda leaf-feeding injury and larval survival which affects maize grain yield. Leaf-feeding injury of S. frugiperda was 29% lower on homozygous hybrids relative to their isogenic hemizygous versions. Isogenic homozygous and hemizygous hybrids did not differ in grain yield in this study. S. frugiperda survivorship on homozygous hybrids was significantly lower than on their hemizygous isogenic versions (16.9% and 38.5%, respectively). Homozygous hybrids presented higher Cry1F, Cry1A.105, and Cry2Ab2 protein expression levels relative to their isogenic hemizygous versions (approximately 1.5-, 2.0-, and 2.5-fold, respectively). The Bt maize event TC1507 x MON89034 x NK603 in a homozygous state increases Bt protein expression levels and the control of S. frugiperda. Therefore, the deployment of homozygous transgenic maize hybrids to farmers is more desirable than the hemizygous versions.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)São Paulo State University (Unesp) School of Agricultural and Veterinarian SciencesCorteva Agriscience IndianópolisSão Paulo State University (Unesp) School of Agricultural and Veterinarian SciencesCAPES: 001Universidade Estadual Paulista (Unesp)IndianópolisEghrari, Kian [UNESP]Humberto de Brito, AndréBaldassi, Amanda [UNESP]Balbuena, Tiago Santana [UNESP]Fernandes, Odair Aparecido [UNESP]Môro, Gustavo Vitti [UNESP]2019-10-06T17:14:02Z2019-10-06T17:14:02Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cropro.2019.104871Crop Protection, v. 124.0261-2194http://hdl.handle.net/11449/19046210.1016/j.cropro.2019.1048712-s2.0-8506826011414582882877578800000-0003-3489-4754Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCrop Protectioninfo:eu-repo/semantics/openAccess2024-06-07T15:32:47Zoai:repositorio.unesp.br:11449/190462Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-07T15:32:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids |
title |
Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids |
spellingShingle |
Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids Eghrari, Kian [UNESP] Bt protein concentration Fall armyworm Resistance management Zygosity |
title_short |
Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids |
title_full |
Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids |
title_fullStr |
Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids |
title_full_unstemmed |
Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids |
title_sort |
Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids |
author |
Eghrari, Kian [UNESP] |
author_facet |
Eghrari, Kian [UNESP] Humberto de Brito, André Baldassi, Amanda [UNESP] Balbuena, Tiago Santana [UNESP] Fernandes, Odair Aparecido [UNESP] Môro, Gustavo Vitti [UNESP] |
author_role |
author |
author2 |
Humberto de Brito, André Baldassi, Amanda [UNESP] Balbuena, Tiago Santana [UNESP] Fernandes, Odair Aparecido [UNESP] Môro, Gustavo Vitti [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Indianópolis |
dc.contributor.author.fl_str_mv |
Eghrari, Kian [UNESP] Humberto de Brito, André Baldassi, Amanda [UNESP] Balbuena, Tiago Santana [UNESP] Fernandes, Odair Aparecido [UNESP] Môro, Gustavo Vitti [UNESP] |
dc.subject.por.fl_str_mv |
Bt protein concentration Fall armyworm Resistance management Zygosity |
topic |
Bt protein concentration Fall armyworm Resistance management Zygosity |
description |
Spodoptera frugiperda (J.E. Smith) is the main pest of maize in Brazil and its control has been targeted by Bt maize hybrids. Generally, Bt maize hybrids possess the transgenic locus in a hemizygous condition, that is, containing only one copy of the Bt allele. However, studies have shown that maize hybrids with an additional transgenic allele, namely, in a homozygous state, increases transgenic protein expression. Our aim in this study was to evaluate whether the transgenic event TC1507 x MON89034 x NK603 in homozygosis increases Bt protein expression levels and, consequently, reduces S. frugiperda leaf-feeding injury and larval survival which affects maize grain yield. Leaf-feeding injury of S. frugiperda was 29% lower on homozygous hybrids relative to their isogenic hemizygous versions. Isogenic homozygous and hemizygous hybrids did not differ in grain yield in this study. S. frugiperda survivorship on homozygous hybrids was significantly lower than on their hemizygous isogenic versions (16.9% and 38.5%, respectively). Homozygous hybrids presented higher Cry1F, Cry1A.105, and Cry2Ab2 protein expression levels relative to their isogenic hemizygous versions (approximately 1.5-, 2.0-, and 2.5-fold, respectively). The Bt maize event TC1507 x MON89034 x NK603 in a homozygous state increases Bt protein expression levels and the control of S. frugiperda. Therefore, the deployment of homozygous transgenic maize hybrids to farmers is more desirable than the hemizygous versions. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:14:02Z 2019-10-06T17:14:02Z 2019-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cropro.2019.104871 Crop Protection, v. 124. 0261-2194 http://hdl.handle.net/11449/190462 10.1016/j.cropro.2019.104871 2-s2.0-85068260114 1458288287757880 0000-0003-3489-4754 |
url |
http://dx.doi.org/10.1016/j.cropro.2019.104871 http://hdl.handle.net/11449/190462 |
identifier_str_mv |
Crop Protection, v. 124. 0261-2194 10.1016/j.cropro.2019.104871 2-s2.0-85068260114 1458288287757880 0000-0003-3489-4754 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Crop Protection |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650286477115392 |