BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesity
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mrgentox.2018.06.009 http://hdl.handle.net/11449/179956 |
Resumo: | Several findings suggest that in utero stressor stimuli can alter fetal development by promoting transcriptional changes, and predisposing the neonate to diseases later in life. This study aimed to investigate whether a hyperglycemic environment in pregnant women with gestational diabetes mellitus (GDM) is able to cause fetal genetic alterations and predispose neonates to obesity. Transcriptional alteration of SIRT1, TP53 and BCL2 genes, miR-181a (a SIRT1 or BCL2 regulator) and telomere length were evaluated in placental and umbilical-cord blood cells. Healthy (HP; n = 20) and GDM (n = 20) pregnant women and their respective neonates were included in the study. Additionally, obese (n = 20) and eutrophic (n = 20) adults also participated as reference populations. Gene expression data showed down-regulation of BCL2 in umbilical-cord and peripheral blood cells from GDM neonates and obese adults, respectively. The miR-181a was down-regulated only in umbilical-cord blood cells of GDM neonates. Telomere length presented no significant difference. In conclusion, our study demonstrated that the GDM hyperglycemic intrauterine environment promotes transcriptional alterations in BCL2 and miR-181a in neonate umbilical-cord blood cells. Furthermore, both GDM neonates and obese subjects share the same transcriptional alteration in BCL2. Considering the relationship between obesity development and the functions regulated by these two genes, BCL2 and miR-181a could be adopted as potential biomarkers for childhood obesity. However, further study designs are recommended to confirm this hypothesis. |
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BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesityFetal programmingGestational diabetes mellitusHyperglycemiaTelomere lengthSeveral findings suggest that in utero stressor stimuli can alter fetal development by promoting transcriptional changes, and predisposing the neonate to diseases later in life. This study aimed to investigate whether a hyperglycemic environment in pregnant women with gestational diabetes mellitus (GDM) is able to cause fetal genetic alterations and predispose neonates to obesity. Transcriptional alteration of SIRT1, TP53 and BCL2 genes, miR-181a (a SIRT1 or BCL2 regulator) and telomere length were evaluated in placental and umbilical-cord blood cells. Healthy (HP; n = 20) and GDM (n = 20) pregnant women and their respective neonates were included in the study. Additionally, obese (n = 20) and eutrophic (n = 20) adults also participated as reference populations. Gene expression data showed down-regulation of BCL2 in umbilical-cord and peripheral blood cells from GDM neonates and obese adults, respectively. The miR-181a was down-regulated only in umbilical-cord blood cells of GDM neonates. Telomere length presented no significant difference. In conclusion, our study demonstrated that the GDM hyperglycemic intrauterine environment promotes transcriptional alterations in BCL2 and miR-181a in neonate umbilical-cord blood cells. Furthermore, both GDM neonates and obese subjects share the same transcriptional alteration in BCL2. Considering the relationship between obesity development and the functions regulated by these two genes, BCL2 and miR-181a could be adopted as potential biomarkers for childhood obesity. However, further study designs are recommended to confirm this hypothesis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP – São Paulo State University Medical SchoolUNESP – São Paulo State University Bioscience InstituteUNESP – São Paulo State University Medical SchoolUNESP – São Paulo State University Bioscience InstituteFAPESP: 2012/19362-7Universidade Estadual Paulista (Unesp)Marcondes, João Paulo de Castro [UNESP]Andrade, Pablo Felipe Bertolini [UNESP]Sávio, André Luiz Ventura [UNESP]Silveira, Maruhen Amir Datsch [UNESP]Rudge, Marilza Vieira Cunha [UNESP]Salvadori, Daisy Maria Fávero [UNESP]2018-12-11T17:37:27Z2018-12-11T17:37:27Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.mrgentox.2018.06.009Mutation Research - Genetic Toxicology and Environmental Mutagenesis.1879-35921383-5718http://hdl.handle.net/11449/17995610.1016/j.mrgentox.2018.06.0092-s2.0-850485378412-s2.0-85048537841.pdf6758680388835078Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMutation Research - Genetic Toxicology and Environmental Mutagenesis0,747info:eu-repo/semantics/openAccess2024-09-03T13:17:53Zoai:repositorio.unesp.br:11449/179956Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:17:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesity |
title |
BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesity |
spellingShingle |
BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesity Marcondes, João Paulo de Castro [UNESP] Fetal programming Gestational diabetes mellitus Hyperglycemia Telomere length |
title_short |
BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesity |
title_full |
BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesity |
title_fullStr |
BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesity |
title_full_unstemmed |
BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesity |
title_sort |
BCL2 and miR-181a transcriptional alterations in umbilical-cord blood cells can be putative biomarkers for obesity |
author |
Marcondes, João Paulo de Castro [UNESP] |
author_facet |
Marcondes, João Paulo de Castro [UNESP] Andrade, Pablo Felipe Bertolini [UNESP] Sávio, André Luiz Ventura [UNESP] Silveira, Maruhen Amir Datsch [UNESP] Rudge, Marilza Vieira Cunha [UNESP] Salvadori, Daisy Maria Fávero [UNESP] |
author_role |
author |
author2 |
Andrade, Pablo Felipe Bertolini [UNESP] Sávio, André Luiz Ventura [UNESP] Silveira, Maruhen Amir Datsch [UNESP] Rudge, Marilza Vieira Cunha [UNESP] Salvadori, Daisy Maria Fávero [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Marcondes, João Paulo de Castro [UNESP] Andrade, Pablo Felipe Bertolini [UNESP] Sávio, André Luiz Ventura [UNESP] Silveira, Maruhen Amir Datsch [UNESP] Rudge, Marilza Vieira Cunha [UNESP] Salvadori, Daisy Maria Fávero [UNESP] |
dc.subject.por.fl_str_mv |
Fetal programming Gestational diabetes mellitus Hyperglycemia Telomere length |
topic |
Fetal programming Gestational diabetes mellitus Hyperglycemia Telomere length |
description |
Several findings suggest that in utero stressor stimuli can alter fetal development by promoting transcriptional changes, and predisposing the neonate to diseases later in life. This study aimed to investigate whether a hyperglycemic environment in pregnant women with gestational diabetes mellitus (GDM) is able to cause fetal genetic alterations and predispose neonates to obesity. Transcriptional alteration of SIRT1, TP53 and BCL2 genes, miR-181a (a SIRT1 or BCL2 regulator) and telomere length were evaluated in placental and umbilical-cord blood cells. Healthy (HP; n = 20) and GDM (n = 20) pregnant women and their respective neonates were included in the study. Additionally, obese (n = 20) and eutrophic (n = 20) adults also participated as reference populations. Gene expression data showed down-regulation of BCL2 in umbilical-cord and peripheral blood cells from GDM neonates and obese adults, respectively. The miR-181a was down-regulated only in umbilical-cord blood cells of GDM neonates. Telomere length presented no significant difference. In conclusion, our study demonstrated that the GDM hyperglycemic intrauterine environment promotes transcriptional alterations in BCL2 and miR-181a in neonate umbilical-cord blood cells. Furthermore, both GDM neonates and obese subjects share the same transcriptional alteration in BCL2. Considering the relationship between obesity development and the functions regulated by these two genes, BCL2 and miR-181a could be adopted as potential biomarkers for childhood obesity. However, further study designs are recommended to confirm this hypothesis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:37:27Z 2018-12-11T17:37:27Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mrgentox.2018.06.009 Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 1879-3592 1383-5718 http://hdl.handle.net/11449/179956 10.1016/j.mrgentox.2018.06.009 2-s2.0-85048537841 2-s2.0-85048537841.pdf 6758680388835078 |
url |
http://dx.doi.org/10.1016/j.mrgentox.2018.06.009 http://hdl.handle.net/11449/179956 |
identifier_str_mv |
Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 1879-3592 1383-5718 10.1016/j.mrgentox.2018.06.009 2-s2.0-85048537841 2-s2.0-85048537841.pdf 6758680388835078 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mutation Research - Genetic Toxicology and Environmental Mutagenesis 0,747 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021398874161152 |