Estudo hodológico e neuroquímico das aferências da região noradrenérgica A5 aos neurônios da raiz coclear

• Autor(a) principal: Martins, Marina Galleazzo [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/124267; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2015-06-15/000829969.pdf
• topic: Ciclo estral; Traçadores (Biologia); Imuno-histoquímica; Ratos Wistar; Tronco encefalico; Estrous Cycle

The coclear root neurons (CRN) are in the coclear portion of the vestibulococlear nerve and they are the first neurons in the central nervous system to receive auditory information from the coclea. They are conected with sensorimotor gating centers in the brainstem and take part in the pathway of the acoustic startle reflex (ASR), which is formed by spiral ganglion neurons, the reticular pontine caudal nucleus and the spinal cord motoneurons. However, the inputs to CRN are unknown and their roles in the ASR and its modulations as the prepulse inhibition (PPI) are ignored. The changes in the ASR and in PPI, can be used as diagnostic methods in neurodegenerative and psychiatric disorders, such as Parkinson and squizophrenia. The sources and neurochemical carachteristics of the afferents to CRN have been studied, but none has evalueted its funtional aspects. Between those sources of afferents, previus studies had identified the area of the noradrenergic group A5. The confirmation of this input source and its neurochemical identity are the first goal of this work. On the other hand, to evaluate the ASR and PPI in animal models, it is indispensable the standardization of a suitable protocol since there are not enough data that correlationate the efficiency of the measurements with the characteristics of stimuli used. Hence, the second goal of this work is to standardize a protocol for ASR and PPI measurement. The results provided will be essential for future experiments on the avaliation of selective lesions in the noradrenergic A5 group