Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals

Detalhes bibliográficos
Autor(a) principal: Castro, Jonathan Pena
Data de Publicação: 2019
Outros Autores: Hattori, Ricardo Shohei, Yoshinaga, Tulio Teruo, Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP], Ruiz-Ruano, Francisco J., Foresti, Fausto [UNESP], Santos, Mateus Henrique, Almeida, Mara Cristina de, Moreira-Filho, Orlando, Artoni, Roberto Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/genes10110909
http://hdl.handle.net/11449/196406
Resumo: The species complex Astyanax scabripinnis is one of the most studied with respect to origin, distribution, and frequency of B chromosomes, and is considered a model organism for evolutionary studies. Research using population inferences about the occurrence and frequency of the B chromosome shows seasonal variation between sexes, which is associated with the presence of this supernumerary element. We hypothesized that the B chromosome could influence the sex ratio of these animals. Based on this assumption, the present work aimed to investigate if differences exist among levels of gene expression with qRT-PCR of the amh (associated with testicular differentiation) and foxl2a (associated with ovarian differentiation) genes between B-carrier and non-B-carrier individuals. The results showed that for the amh gene, the difference in expression between animals with B chromosomes was not accentuated compared to that in animals without this chromosome. Expression of foxl2a in B-carrier females, however, was reduced by 73.56% compared to females that lacked the B chromosome. Males had no difference in expression of the amh and foxl2a genes between carriers and non-carriers of the B chromosome. Results indicate that the presence of B chromosomes is correlated with the differential expression of sex-associated genes. An analysis of these results integrated with data from other studies on the reproductive cycle in the same species reveals that this difference in expression may be expanding the reproductive cycle of the species.
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spelling Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individualsfishessupernumerary chromosomessexual differentiationfoxl2aamhThe species complex Astyanax scabripinnis is one of the most studied with respect to origin, distribution, and frequency of B chromosomes, and is considered a model organism for evolutionary studies. Research using population inferences about the occurrence and frequency of the B chromosome shows seasonal variation between sexes, which is associated with the presence of this supernumerary element. We hypothesized that the B chromosome could influence the sex ratio of these animals. Based on this assumption, the present work aimed to investigate if differences exist among levels of gene expression with qRT-PCR of the amh (associated with testicular differentiation) and foxl2a (associated with ovarian differentiation) genes between B-carrier and non-B-carrier individuals. The results showed that for the amh gene, the difference in expression between animals with B chromosomes was not accentuated compared to that in animals without this chromosome. Expression of foxl2a in B-carrier females, however, was reduced by 73.56% compared to females that lacked the B chromosome. Males had no difference in expression of the amh and foxl2a genes between carriers and non-carriers of the B chromosome. Results indicate that the presence of B chromosomes is correlated with the differential expression of sex-associated genes. An analysis of these results integrated with data from other studies on the reproductive cycle in the same species reveals that this difference in expression may be expanding the reproductive cycle of the species.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao Araucaria (Fundacao Araucaria de Apoio ao Desenvolvimento Cientifico e Tecnologico do Estado do Parana)Univ Fed Sao Carlos, Dept Genet & Evolucao, Programa Posgrad Biol Evolut & Genet Mol, Rodovia Washington Luis,Km 235, BR-13565905 Sao Carlos, SP, BrazilUPD CJ APTA SAA, Estacao Expt Salmonicultura Campos do Jordao, BR-12460000 Sao Paulo, SP, BrazilUniv Sao Paulo, Fac Med Vet & Zootecnia, Dept Cirurgia, Rua Prof Orlando Marque Paiva, BR-05508270 Sao Paulo, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP, BrazilUppsala Univ, Evolutionary Biol Ctr, Dept Ecol & Genet, SE-75236 Uppsala, SwedenUniv Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Programa Posgrad Biol Evolut, Ave Carlos Cavalcanti 4748, BR-84030900 Ponta Grossa, PR, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP, BrazilCNPq: 407187/2016-2CAPES: 001Fundacao Araucaria (Fundacao Araucaria de Apoio ao Desenvolvimento Cientifico e Tecnologico do Estado do Parana): 16174MdpiUniversidade Federal de São Carlos (UFSCar)UPD CJ APTA SAAUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Uppsala UnivUniversidade Estadual de Ponta Grossa (UEPG)Castro, Jonathan PenaHattori, Ricardo ShoheiYoshinaga, Tulio TeruoZerbinato de Andrade Silva, Duilio Mazzoni [UNESP]Ruiz-Ruano, Francisco J.Foresti, Fausto [UNESP]Santos, Mateus HenriqueAlmeida, Mara Cristina deMoreira-Filho, OrlandoArtoni, Roberto Ferreira2020-12-10T19:43:51Z2020-12-10T19:43:51Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.3390/genes10110909Genes. Basel: Mdpi, v. 10, n. 11, 12 p., 2019.http://hdl.handle.net/11449/19640610.3390/genes10110909WOS:000502296000075Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenesinfo:eu-repo/semantics/openAccess2021-10-22T21:54:39Zoai:repositorio.unesp.br:11449/196406Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:21:36.129746Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
title Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
spellingShingle Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
Castro, Jonathan Pena
fishes
supernumerary chromosomes
sexual differentiation
foxl2a
amh
title_short Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
title_full Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
title_fullStr Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
title_full_unstemmed Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
title_sort Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
author Castro, Jonathan Pena
author_facet Castro, Jonathan Pena
Hattori, Ricardo Shohei
Yoshinaga, Tulio Teruo
Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP]
Ruiz-Ruano, Francisco J.
Foresti, Fausto [UNESP]
Santos, Mateus Henrique
Almeida, Mara Cristina de
Moreira-Filho, Orlando
Artoni, Roberto Ferreira
author_role author
author2 Hattori, Ricardo Shohei
Yoshinaga, Tulio Teruo
Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP]
Ruiz-Ruano, Francisco J.
Foresti, Fausto [UNESP]
Santos, Mateus Henrique
Almeida, Mara Cristina de
Moreira-Filho, Orlando
Artoni, Roberto Ferreira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Carlos (UFSCar)
UPD CJ APTA SAA
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Uppsala Univ
Universidade Estadual de Ponta Grossa (UEPG)
dc.contributor.author.fl_str_mv Castro, Jonathan Pena
Hattori, Ricardo Shohei
Yoshinaga, Tulio Teruo
Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP]
Ruiz-Ruano, Francisco J.
Foresti, Fausto [UNESP]
Santos, Mateus Henrique
Almeida, Mara Cristina de
Moreira-Filho, Orlando
Artoni, Roberto Ferreira
dc.subject.por.fl_str_mv fishes
supernumerary chromosomes
sexual differentiation
foxl2a
amh
topic fishes
supernumerary chromosomes
sexual differentiation
foxl2a
amh
description The species complex Astyanax scabripinnis is one of the most studied with respect to origin, distribution, and frequency of B chromosomes, and is considered a model organism for evolutionary studies. Research using population inferences about the occurrence and frequency of the B chromosome shows seasonal variation between sexes, which is associated with the presence of this supernumerary element. We hypothesized that the B chromosome could influence the sex ratio of these animals. Based on this assumption, the present work aimed to investigate if differences exist among levels of gene expression with qRT-PCR of the amh (associated with testicular differentiation) and foxl2a (associated with ovarian differentiation) genes between B-carrier and non-B-carrier individuals. The results showed that for the amh gene, the difference in expression between animals with B chromosomes was not accentuated compared to that in animals without this chromosome. Expression of foxl2a in B-carrier females, however, was reduced by 73.56% compared to females that lacked the B chromosome. Males had no difference in expression of the amh and foxl2a genes between carriers and non-carriers of the B chromosome. Results indicate that the presence of B chromosomes is correlated with the differential expression of sex-associated genes. An analysis of these results integrated with data from other studies on the reproductive cycle in the same species reveals that this difference in expression may be expanding the reproductive cycle of the species.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-01
2020-12-10T19:43:51Z
2020-12-10T19:43:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/genes10110909
Genes. Basel: Mdpi, v. 10, n. 11, 12 p., 2019.
http://hdl.handle.net/11449/196406
10.3390/genes10110909
WOS:000502296000075
url http://dx.doi.org/10.3390/genes10110909
http://hdl.handle.net/11449/196406
identifier_str_mv Genes. Basel: Mdpi, v. 10, n. 11, 12 p., 2019.
10.3390/genes10110909
WOS:000502296000075
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genes
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv Mdpi
publisher.none.fl_str_mv Mdpi
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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