Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/genes10110909 http://hdl.handle.net/11449/196406 |
Resumo: | The species complex Astyanax scabripinnis is one of the most studied with respect to origin, distribution, and frequency of B chromosomes, and is considered a model organism for evolutionary studies. Research using population inferences about the occurrence and frequency of the B chromosome shows seasonal variation between sexes, which is associated with the presence of this supernumerary element. We hypothesized that the B chromosome could influence the sex ratio of these animals. Based on this assumption, the present work aimed to investigate if differences exist among levels of gene expression with qRT-PCR of the amh (associated with testicular differentiation) and foxl2a (associated with ovarian differentiation) genes between B-carrier and non-B-carrier individuals. The results showed that for the amh gene, the difference in expression between animals with B chromosomes was not accentuated compared to that in animals without this chromosome. Expression of foxl2a in B-carrier females, however, was reduced by 73.56% compared to females that lacked the B chromosome. Males had no difference in expression of the amh and foxl2a genes between carriers and non-carriers of the B chromosome. Results indicate that the presence of B chromosomes is correlated with the differential expression of sex-associated genes. An analysis of these results integrated with data from other studies on the reproductive cycle in the same species reveals that this difference in expression may be expanding the reproductive cycle of the species. |
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Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individualsfishessupernumerary chromosomessexual differentiationfoxl2aamhThe species complex Astyanax scabripinnis is one of the most studied with respect to origin, distribution, and frequency of B chromosomes, and is considered a model organism for evolutionary studies. Research using population inferences about the occurrence and frequency of the B chromosome shows seasonal variation between sexes, which is associated with the presence of this supernumerary element. We hypothesized that the B chromosome could influence the sex ratio of these animals. Based on this assumption, the present work aimed to investigate if differences exist among levels of gene expression with qRT-PCR of the amh (associated with testicular differentiation) and foxl2a (associated with ovarian differentiation) genes between B-carrier and non-B-carrier individuals. The results showed that for the amh gene, the difference in expression between animals with B chromosomes was not accentuated compared to that in animals without this chromosome. Expression of foxl2a in B-carrier females, however, was reduced by 73.56% compared to females that lacked the B chromosome. Males had no difference in expression of the amh and foxl2a genes between carriers and non-carriers of the B chromosome. Results indicate that the presence of B chromosomes is correlated with the differential expression of sex-associated genes. An analysis of these results integrated with data from other studies on the reproductive cycle in the same species reveals that this difference in expression may be expanding the reproductive cycle of the species.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao Araucaria (Fundacao Araucaria de Apoio ao Desenvolvimento Cientifico e Tecnologico do Estado do Parana)Univ Fed Sao Carlos, Dept Genet & Evolucao, Programa Posgrad Biol Evolut & Genet Mol, Rodovia Washington Luis,Km 235, BR-13565905 Sao Carlos, SP, BrazilUPD CJ APTA SAA, Estacao Expt Salmonicultura Campos do Jordao, BR-12460000 Sao Paulo, SP, BrazilUniv Sao Paulo, Fac Med Vet & Zootecnia, Dept Cirurgia, Rua Prof Orlando Marque Paiva, BR-05508270 Sao Paulo, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP, BrazilUppsala Univ, Evolutionary Biol Ctr, Dept Ecol & Genet, SE-75236 Uppsala, SwedenUniv Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Programa Posgrad Biol Evolut, Ave Carlos Cavalcanti 4748, BR-84030900 Ponta Grossa, PR, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP, BrazilCNPq: 407187/2016-2CAPES: 001Fundacao Araucaria (Fundacao Araucaria de Apoio ao Desenvolvimento Cientifico e Tecnologico do Estado do Parana): 16174MdpiUniversidade Federal de São Carlos (UFSCar)UPD CJ APTA SAAUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Uppsala UnivUniversidade Estadual de Ponta Grossa (UEPG)Castro, Jonathan PenaHattori, Ricardo ShoheiYoshinaga, Tulio TeruoZerbinato de Andrade Silva, Duilio Mazzoni [UNESP]Ruiz-Ruano, Francisco J.Foresti, Fausto [UNESP]Santos, Mateus HenriqueAlmeida, Mara Cristina deMoreira-Filho, OrlandoArtoni, Roberto Ferreira2020-12-10T19:43:51Z2020-12-10T19:43:51Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.3390/genes10110909Genes. Basel: Mdpi, v. 10, n. 11, 12 p., 2019.http://hdl.handle.net/11449/19640610.3390/genes10110909WOS:000502296000075Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenesinfo:eu-repo/semantics/openAccess2021-10-22T21:54:39Zoai:repositorio.unesp.br:11449/196406Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:21:36.129746Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals |
title |
Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals |
spellingShingle |
Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals Castro, Jonathan Pena fishes supernumerary chromosomes sexual differentiation foxl2a amh |
title_short |
Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals |
title_full |
Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals |
title_fullStr |
Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals |
title_full_unstemmed |
Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals |
title_sort |
Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals |
author |
Castro, Jonathan Pena |
author_facet |
Castro, Jonathan Pena Hattori, Ricardo Shohei Yoshinaga, Tulio Teruo Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP] Ruiz-Ruano, Francisco J. Foresti, Fausto [UNESP] Santos, Mateus Henrique Almeida, Mara Cristina de Moreira-Filho, Orlando Artoni, Roberto Ferreira |
author_role |
author |
author2 |
Hattori, Ricardo Shohei Yoshinaga, Tulio Teruo Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP] Ruiz-Ruano, Francisco J. Foresti, Fausto [UNESP] Santos, Mateus Henrique Almeida, Mara Cristina de Moreira-Filho, Orlando Artoni, Roberto Ferreira |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) UPD CJ APTA SAA Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Uppsala Univ Universidade Estadual de Ponta Grossa (UEPG) |
dc.contributor.author.fl_str_mv |
Castro, Jonathan Pena Hattori, Ricardo Shohei Yoshinaga, Tulio Teruo Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP] Ruiz-Ruano, Francisco J. Foresti, Fausto [UNESP] Santos, Mateus Henrique Almeida, Mara Cristina de Moreira-Filho, Orlando Artoni, Roberto Ferreira |
dc.subject.por.fl_str_mv |
fishes supernumerary chromosomes sexual differentiation foxl2a amh |
topic |
fishes supernumerary chromosomes sexual differentiation foxl2a amh |
description |
The species complex Astyanax scabripinnis is one of the most studied with respect to origin, distribution, and frequency of B chromosomes, and is considered a model organism for evolutionary studies. Research using population inferences about the occurrence and frequency of the B chromosome shows seasonal variation between sexes, which is associated with the presence of this supernumerary element. We hypothesized that the B chromosome could influence the sex ratio of these animals. Based on this assumption, the present work aimed to investigate if differences exist among levels of gene expression with qRT-PCR of the amh (associated with testicular differentiation) and foxl2a (associated with ovarian differentiation) genes between B-carrier and non-B-carrier individuals. The results showed that for the amh gene, the difference in expression between animals with B chromosomes was not accentuated compared to that in animals without this chromosome. Expression of foxl2a in B-carrier females, however, was reduced by 73.56% compared to females that lacked the B chromosome. Males had no difference in expression of the amh and foxl2a genes between carriers and non-carriers of the B chromosome. Results indicate that the presence of B chromosomes is correlated with the differential expression of sex-associated genes. An analysis of these results integrated with data from other studies on the reproductive cycle in the same species reveals that this difference in expression may be expanding the reproductive cycle of the species. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-01 2020-12-10T19:43:51Z 2020-12-10T19:43:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/genes10110909 Genes. Basel: Mdpi, v. 10, n. 11, 12 p., 2019. http://hdl.handle.net/11449/196406 10.3390/genes10110909 WOS:000502296000075 |
url |
http://dx.doi.org/10.3390/genes10110909 http://hdl.handle.net/11449/196406 |
identifier_str_mv |
Genes. Basel: Mdpi, v. 10, n. 11, 12 p., 2019. 10.3390/genes10110909 WOS:000502296000075 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genes |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129057985921024 |