Atividade viral do vírus respiratório sincicial bovino (brsv) em bezerros leiteiros

• Autor(a) principal: Affonso, Ingrid Bortolin [UNESP]
• Data de Publicação: 2012
• Outros Autores: Gatti, Sandra Possebon [UNESP], de Medeiros, Andréa Souza Ramos [UNESP], Buzinaro, Maria da Glória [UNESP], Samara, Samir Issa [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.5216/cab.v13i2.10318; http://hdl.handle.net/11449/73259
• topic: Antimicrobial therapy; Dairy goats; Minimal bactericidal concentration; Natural products; Bacteria (microorganisms); Bovine respiratory syncytial virus; Capra; Capra hircus; Respiratory syncytial virus; Staphylococcus

The purpose of the present study was to evaluate the antimicrobial activity of propolis extracts diluted in different solvents against bacteria from Staphylococcus genus. The study was performed in the Immunology and Microbiology Laboratory from Universidade Federal do Vale do São Francisco. The propolis extracts were prepared using brown propolis diluted in different solvents such as chloroform, methanol, ethyl acetate and grain alcohol. In order to determine the antimicrobial potential of extracts, agar well diffusion method was used, with controls for each diluent. After that, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods were used. All tests were performed in triplicate. In the agar well diffusion test, the measurements of the inhibition zone for propolis extract were as follows: grain alcohol and propolis (2.88mm), methanol and propolis (2.41mm), chloroform and propolis (2.40mm) and ethyl acetate and propolis (0.83mm). The MBC of propolis extracts in different solvents were 93.75 μg/mL for grain alcohol, 375 μg/mL for chloroform and methanol and 3,000 μg/ml for ethyl acetate. Statistically significant differences were achieved comparing the inhibition zones of propolis diluted in grain alcohol and ethyl acetate (2.88 and 0.83 mm, respectively). Considering the low cost of therapy and the activity of the propolis against caprine mastitis pathogens, other studies regarding in vivo activity and chemical characterization are necessary, in addition to evaluation of the toxicological aspects of propolis extracts.