Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinos
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-8478cr20180078 http://hdl.handle.net/11449/164488 |
Resumo: | Preservation and use of spermatozoa that have been recovered after death can extend the use of genetically superior animals. The objective of this study was to evaluate the maximum period for which ovine spermatozoa could be successfully stored in refrigerated dilution medium post-mortem, with or without added seminal plasma. Three samples of spermatozoa collected in an artificial vagina from 10 rams, or from the tails of four epididymes from the same rams at the time of death (G0) and six (G6), twelve (G12), twenty-four (G24) and fortyeight (G48) hours after death were used. After recovery, the spermatozoa were refrigerated at 5 degrees C in either control medium (CM) or control medium plus 20% homologous seminal plasma (SP) and evaluated for 72 hours from the start of refrigeration. The G48 samples had a lower(P < 0.05) total motility (TM) and plasma membrane integrity in the hyposmotic test (HOST) than the other groups evaluated at all analyzed times. The TM decreased (P < 0.05) after 24 hours of cooling in semen collected in AV, at G0 and G24 and after 48 hours of refrigeration in G6 and G12. The TM and HOST integrity and sperm morphology did not differ between samples refrigerated in CM or SP. In conclusion, it is possible to collect epididymal spermatozoa up to 24 hours after death. Sperm viability can be prolonged fora further 48 hours by refrigeration. However, total motility decreases from 24 hours after refrigeration and the supplementation of 20% seminal plasma to the extender has no effect on spermatozoa longevity. |
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Repositório Institucional da UNESP |
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Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinosrefrigerationepididymispost mortemPreservation and use of spermatozoa that have been recovered after death can extend the use of genetically superior animals. The objective of this study was to evaluate the maximum period for which ovine spermatozoa could be successfully stored in refrigerated dilution medium post-mortem, with or without added seminal plasma. Three samples of spermatozoa collected in an artificial vagina from 10 rams, or from the tails of four epididymes from the same rams at the time of death (G0) and six (G6), twelve (G12), twenty-four (G24) and fortyeight (G48) hours after death were used. After recovery, the spermatozoa were refrigerated at 5 degrees C in either control medium (CM) or control medium plus 20% homologous seminal plasma (SP) and evaluated for 72 hours from the start of refrigeration. The G48 samples had a lower(P < 0.05) total motility (TM) and plasma membrane integrity in the hyposmotic test (HOST) than the other groups evaluated at all analyzed times. The TM decreased (P < 0.05) after 24 hours of cooling in semen collected in AV, at G0 and G24 and after 48 hours of refrigeration in G6 and G12. The TM and HOST integrity and sperm morphology did not differ between samples refrigerated in CM or SP. In conclusion, it is possible to collect epididymal spermatozoa up to 24 hours after death. Sperm viability can be prolonged fora further 48 hours by refrigeration. However, total motility decreases from 24 hours after refrigeration and the supplementation of 20% seminal plasma to the extender has no effect on spermatozoa longevity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Parana UFPR, Dept Tecnol, Engn Bioproc & Biotecnol, Curitiba, Parana, BrazilCent Alamos Genet, Balsa Nova, PR, BrazilUniv Fed Parana UFPR, Ciencia Vet, Dept Med Vet, Curitiba, Parana, BrazilPontiticia Univ Catolica Parana PUCPR, Dept Med Vet, Estudos Ciencia Anim, Curitiba, Parana, BrazilUniv Estadual Paulista UNESP, FMVZ, Dept Reprod Anim & Radiol Vet, Botucatu, SP, BrazilUniv Estadual Paulista UNESP, FMVZ, Dept Reprod Anim & Radiol Vet, Botucatu, SP, BrazilUniv Federal Santa MariaUniversidade Federal do Paraná (UFPR)Cent Alamos GenetPontiticia Univ Catolica Parana PUCPRUniversidade Estadual Paulista (Unesp)Bergstein-Galan, Tacia ComesWeiss, Romildo RomualdoRodrigues Barbosa, Thiago SalvatiKozicki, Luiz ErnandesBicudo, Sony Dimas [UNESP]2018-11-26T17:54:45Z2018-11-26T17:54:45Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1590/0103-8478cr20180078Ciencia Rural. Santa Maria: Univ Federal Santa Maria, v. 48, n. 8, 7 p., 2018.0103-8478http://hdl.handle.net/11449/16448810.1590/0103-8478cr20180078S0103-84782018000800700WOS:000440795100001S0103-84782018000800700.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Rural0,337info:eu-repo/semantics/openAccess2024-09-09T14:00:46Zoai:repositorio.unesp.br:11449/164488Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:00:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinos |
title |
Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinos |
spellingShingle |
Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinos Bergstein-Galan, Tacia Comes refrigeration epididymis post mortem |
title_short |
Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinos |
title_full |
Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinos |
title_fullStr |
Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinos |
title_full_unstemmed |
Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinos |
title_sort |
Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimarios ovinos |
author |
Bergstein-Galan, Tacia Comes |
author_facet |
Bergstein-Galan, Tacia Comes Weiss, Romildo Romualdo Rodrigues Barbosa, Thiago Salvati Kozicki, Luiz Ernandes Bicudo, Sony Dimas [UNESP] |
author_role |
author |
author2 |
Weiss, Romildo Romualdo Rodrigues Barbosa, Thiago Salvati Kozicki, Luiz Ernandes Bicudo, Sony Dimas [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná (UFPR) Cent Alamos Genet Pontiticia Univ Catolica Parana PUCPR Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bergstein-Galan, Tacia Comes Weiss, Romildo Romualdo Rodrigues Barbosa, Thiago Salvati Kozicki, Luiz Ernandes Bicudo, Sony Dimas [UNESP] |
dc.subject.por.fl_str_mv |
refrigeration epididymis post mortem |
topic |
refrigeration epididymis post mortem |
description |
Preservation and use of spermatozoa that have been recovered after death can extend the use of genetically superior animals. The objective of this study was to evaluate the maximum period for which ovine spermatozoa could be successfully stored in refrigerated dilution medium post-mortem, with or without added seminal plasma. Three samples of spermatozoa collected in an artificial vagina from 10 rams, or from the tails of four epididymes from the same rams at the time of death (G0) and six (G6), twelve (G12), twenty-four (G24) and fortyeight (G48) hours after death were used. After recovery, the spermatozoa were refrigerated at 5 degrees C in either control medium (CM) or control medium plus 20% homologous seminal plasma (SP) and evaluated for 72 hours from the start of refrigeration. The G48 samples had a lower(P < 0.05) total motility (TM) and plasma membrane integrity in the hyposmotic test (HOST) than the other groups evaluated at all analyzed times. The TM decreased (P < 0.05) after 24 hours of cooling in semen collected in AV, at G0 and G24 and after 48 hours of refrigeration in G6 and G12. The TM and HOST integrity and sperm morphology did not differ between samples refrigerated in CM or SP. In conclusion, it is possible to collect epididymal spermatozoa up to 24 hours after death. Sperm viability can be prolonged fora further 48 hours by refrigeration. However, total motility decreases from 24 hours after refrigeration and the supplementation of 20% seminal plasma to the extender has no effect on spermatozoa longevity. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:54:45Z 2018-11-26T17:54:45Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-8478cr20180078 Ciencia Rural. Santa Maria: Univ Federal Santa Maria, v. 48, n. 8, 7 p., 2018. 0103-8478 http://hdl.handle.net/11449/164488 10.1590/0103-8478cr20180078 S0103-84782018000800700 WOS:000440795100001 S0103-84782018000800700.pdf |
url |
http://dx.doi.org/10.1590/0103-8478cr20180078 http://hdl.handle.net/11449/164488 |
identifier_str_mv |
Ciencia Rural. Santa Maria: Univ Federal Santa Maria, v. 48, n. 8, 7 p., 2018. 0103-8478 10.1590/0103-8478cr20180078 S0103-84782018000800700 WOS:000440795100001 S0103-84782018000800700.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ciencia Rural 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Federal Santa Maria |
publisher.none.fl_str_mv |
Univ Federal Santa Maria |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021314638905344 |