Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2020.105001 http://hdl.handle.net/11449/206954 |
Resumo: | Objective: To evaluate the effects of combination of treatments with fluoridated toothpastes supplemented with sodium trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®), on the remineralization of dental enamel. Design: Enamel blocks with artificial caries were randomly allocated into six groups (n = 12), according to the toothpastes: 1) without F-TMP-MI Paste Plus® (Placebo); 2) 1100 ppm F (1100 F), 3) MI Paste Plus®, 4) 1100 F + MI Paste Plus® (1100 F-MI Paste Plus®), 5) 1100 F + 3% TMP (1100 F-TMP) and 6) 1100 F-TMP + MI Paste Plus® (1100 F-TMP-MI Paste Plus®). Blocks were treated 2×/day with slurries of toothpastes (1 min). Furthermore, groups 4 and 6 received the application of MI Paste Plus® for 3 min. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM), confocal laser scanning microscopy (LSCM), scanning electron microscopy (SEM), fluoride (F), calcium (Ca), phosphorus (P) concentrations in the enamel were determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p < 0.001). Results: 1100 F-TMP-MI Paste Plus® group showed the best results of %SHR, ΔKHN and PLM (p < 0.001). F concentration was similar between the 1100 F, 1100 F-MI Paste Plus®, and 1100 F-TMP-MI Paste Plus® groups (p > 0.001). 1100 F-TMP-MI Paste Plus® group showed the highest concentration of Ca and P in the enamel (p < 0.001). Conclusion: The association of 1100 F-TMP and MI Paste Plus® led to a significant increase in the remineralization of initial carious lesions. |
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Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro studyCPP-ACPDental enamelFluoridePhosphateRemineralizationObjective: To evaluate the effects of combination of treatments with fluoridated toothpastes supplemented with sodium trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®), on the remineralization of dental enamel. Design: Enamel blocks with artificial caries were randomly allocated into six groups (n = 12), according to the toothpastes: 1) without F-TMP-MI Paste Plus® (Placebo); 2) 1100 ppm F (1100 F), 3) MI Paste Plus®, 4) 1100 F + MI Paste Plus® (1100 F-MI Paste Plus®), 5) 1100 F + 3% TMP (1100 F-TMP) and 6) 1100 F-TMP + MI Paste Plus® (1100 F-TMP-MI Paste Plus®). Blocks were treated 2×/day with slurries of toothpastes (1 min). Furthermore, groups 4 and 6 received the application of MI Paste Plus® for 3 min. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM), confocal laser scanning microscopy (LSCM), scanning electron microscopy (SEM), fluoride (F), calcium (Ca), phosphorus (P) concentrations in the enamel were determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p < 0.001). Results: 1100 F-TMP-MI Paste Plus® group showed the best results of %SHR, ΔKHN and PLM (p < 0.001). F concentration was similar between the 1100 F, 1100 F-MI Paste Plus®, and 1100 F-TMP-MI Paste Plus® groups (p > 0.001). 1100 F-TMP-MI Paste Plus® group showed the highest concentration of Ca and P in the enamel (p < 0.001). Conclusion: The association of 1100 F-TMP and MI Paste Plus® led to a significant increase in the remineralization of initial carious lesions.São Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, Rua José Bonifácio 1193 AraçatubaFederal University of São Carlos - UFSCar Department of Materials EngineeringNorthwestern University Feinberg School of Medicine Ann and Robert Lurie Children's HospitalSchool of Dentistry University of Ribeirão Preto - UNAERPSão Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, Rua José Bonifácio 1193 AraçatubaUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Ann and Robert Lurie Children's HospitalUniversity of Ribeirão Preto - UNAERPGonçalves, Francyenne Maira Castro [UNESP]Delbem, Alberto Carlos Botazzo [UNESP]Gomes, Leonardo FernandesEmerenciano, Nayara Gonçalves [UNESP]Pessan, Juliano Pelim [UNESP]Romero, Giovana Dornelas Azevedo [UNESP]Cannon, Mark L.Danelon, Marcelle [UNESP]2021-06-25T10:46:36Z2021-06-25T10:46:36Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2020.105001Archives of Oral Biology, v. 122.1879-15060003-9969http://hdl.handle.net/11449/20695410.1016/j.archoralbio.2020.1050012-s2.0-85097477234Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2024-09-19T18:31:42Zoai:repositorio.unesp.br:11449/206954Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study |
title |
Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study |
spellingShingle |
Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study Gonçalves, Francyenne Maira Castro [UNESP] CPP-ACP Dental enamel Fluoride Phosphate Remineralization |
title_short |
Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study |
title_full |
Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study |
title_fullStr |
Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study |
title_full_unstemmed |
Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study |
title_sort |
Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study |
author |
Gonçalves, Francyenne Maira Castro [UNESP] |
author_facet |
Gonçalves, Francyenne Maira Castro [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Gomes, Leonardo Fernandes Emerenciano, Nayara Gonçalves [UNESP] Pessan, Juliano Pelim [UNESP] Romero, Giovana Dornelas Azevedo [UNESP] Cannon, Mark L. Danelon, Marcelle [UNESP] |
author_role |
author |
author2 |
Delbem, Alberto Carlos Botazzo [UNESP] Gomes, Leonardo Fernandes Emerenciano, Nayara Gonçalves [UNESP] Pessan, Juliano Pelim [UNESP] Romero, Giovana Dornelas Azevedo [UNESP] Cannon, Mark L. Danelon, Marcelle [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) Ann and Robert Lurie Children's Hospital University of Ribeirão Preto - UNAERP |
dc.contributor.author.fl_str_mv |
Gonçalves, Francyenne Maira Castro [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Gomes, Leonardo Fernandes Emerenciano, Nayara Gonçalves [UNESP] Pessan, Juliano Pelim [UNESP] Romero, Giovana Dornelas Azevedo [UNESP] Cannon, Mark L. Danelon, Marcelle [UNESP] |
dc.subject.por.fl_str_mv |
CPP-ACP Dental enamel Fluoride Phosphate Remineralization |
topic |
CPP-ACP Dental enamel Fluoride Phosphate Remineralization |
description |
Objective: To evaluate the effects of combination of treatments with fluoridated toothpastes supplemented with sodium trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®), on the remineralization of dental enamel. Design: Enamel blocks with artificial caries were randomly allocated into six groups (n = 12), according to the toothpastes: 1) without F-TMP-MI Paste Plus® (Placebo); 2) 1100 ppm F (1100 F), 3) MI Paste Plus®, 4) 1100 F + MI Paste Plus® (1100 F-MI Paste Plus®), 5) 1100 F + 3% TMP (1100 F-TMP) and 6) 1100 F-TMP + MI Paste Plus® (1100 F-TMP-MI Paste Plus®). Blocks were treated 2×/day with slurries of toothpastes (1 min). Furthermore, groups 4 and 6 received the application of MI Paste Plus® for 3 min. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM), confocal laser scanning microscopy (LSCM), scanning electron microscopy (SEM), fluoride (F), calcium (Ca), phosphorus (P) concentrations in the enamel were determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p < 0.001). Results: 1100 F-TMP-MI Paste Plus® group showed the best results of %SHR, ΔKHN and PLM (p < 0.001). F concentration was similar between the 1100 F, 1100 F-MI Paste Plus®, and 1100 F-TMP-MI Paste Plus® groups (p > 0.001). 1100 F-TMP-MI Paste Plus® group showed the highest concentration of Ca and P in the enamel (p < 0.001). Conclusion: The association of 1100 F-TMP and MI Paste Plus® led to a significant increase in the remineralization of initial carious lesions. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:46:36Z 2021-06-25T10:46:36Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2020.105001 Archives of Oral Biology, v. 122. 1879-1506 0003-9969 http://hdl.handle.net/11449/206954 10.1016/j.archoralbio.2020.105001 2-s2.0-85097477234 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2020.105001 http://hdl.handle.net/11449/206954 |
identifier_str_mv |
Archives of Oral Biology, v. 122. 1879-1506 0003-9969 10.1016/j.archoralbio.2020.105001 2-s2.0-85097477234 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546445776617472 |