Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel

Detalhes bibliográficos
Autor(a) principal: Sanches, Rafael de Jesus [UNESP]
Data de Publicação: 2009
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/120941
Resumo: The oil and biodiesel are oily substances which, if spilled or thrown out in the environment, can cause serious damages. In this context, bioremediation techniques can be used to the recovering of sites degradated by mineral or vegetable oil, besides them, the biodegradation. However, it is necessary that there are microorganism strains used to degradate the contaminant, previously selected for the fast, efficient and minimum impact remediation. The main objective of this study was to isolate microorganism strains coming from different ecological recesses and verify their potentiality on biodegradating oil and biodiesel. Strains of bacteria, fungi and yeasts were isolated from soils contaminated by hydrocarbons (diesel, gasoline, grease) before, by doing striations on culture plates. On the biodegradation tests, it was used the redox indicator, 2,6-dichlorophenol indophenol (DCPIP), then, after the indicador’s discoloration, the absorbance of the suspension obtained was measured. 46 different microorganism strains were isolated, and the oil, when it was used as a carbon resource, it was degradate easier than the biodiesel. The experiments showed that, in a period, the site impacted by oil spills recovers itself by the biodegradation, in aerobic conditions, by the redox activity.
id UNSP_73b2520287ad2e752d4eebb5ad9c133c
oai_identifier_str oai:repositorio.unesp.br:11449/120941
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodieselMicroorganismosBiorremediaçãoPetróleoBiodegradationBiorremediationOilBiodieselThe oil and biodiesel are oily substances which, if spilled or thrown out in the environment, can cause serious damages. In this context, bioremediation techniques can be used to the recovering of sites degradated by mineral or vegetable oil, besides them, the biodegradation. However, it is necessary that there are microorganism strains used to degradate the contaminant, previously selected for the fast, efficient and minimum impact remediation. The main objective of this study was to isolate microorganism strains coming from different ecological recesses and verify their potentiality on biodegradating oil and biodiesel. Strains of bacteria, fungi and yeasts were isolated from soils contaminated by hydrocarbons (diesel, gasoline, grease) before, by doing striations on culture plates. On the biodegradation tests, it was used the redox indicator, 2,6-dichlorophenol indophenol (DCPIP), then, after the indicador’s discoloration, the absorbance of the suspension obtained was measured. 46 different microorganism strains were isolated, and the oil, when it was used as a carbon resource, it was degradate easier than the biodiesel. The experiments showed that, in a period, the site impacted by oil spills recovers itself by the biodegradation, in aerobic conditions, by the redox activity.O petróleo e o biodiesel são substâncias oleaginosas cujos descartes no meio ambiente podem causar sérios problemas. Nesse contexto, podem-se utilizar técnicas de biorremediação para a recuperação de áreas degradadas com óleos minerais ou vegetais, dentre elas, a biodegradação. Para tanto, é necessário que se tenham linhagens de micro-organismos adaptados à degradação do contaminante, previamente selecionados para que a remediação seja rápida, eficaz e de menor impacto possível. O objetivo central deste trabalho foi isolar linhagens de micro-organismos provenientes de diferentes nichos ecológicos, e verificar também o potencial dos mesmos para biodegradação de petróleo e biodiesel. Foram isoladas linhagens de bactérias, fungos e leveduras provenientes de solos anteriormente contaminados com hidrocarbonetos (diesel, gasolina, graxas), por meio de estriamentos em placas de cultura. Nos testes de biodegradação empregou-se o indicador redox 2,6-diclorofenol indofenol (DCPIP) e mediu-se a absorbância da suspensão obtida, após descoloração do indicador e centrifugação. Isolou-se 46 diferentes biótipos de micro-organismos, sendo que o petróleo, quando usado como fonte de carbono, foi degradado mais facilmente que o biodiesel. Os experimentos demonstraram que, a médio e longo prazo, a área impactada com derrame de óleos se auto recupera pela biodegradação, em condições aeróbias por meio da óxido-redução.Universidade Estadual Paulista (Unesp)Angelis, Dejanira de Franceschi de [UNESP]Universidade Estadual Paulista (Unesp)Sanches, Rafael de Jesus [UNESP]2015-03-23T15:28:12Z2015-03-23T15:28:12Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisvi, 55 f.application/pdfSANCHES, Rafael de Jesus. Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel. 2009. vi, 55 f. Trabalho de conclusão de curso (Engenharia Ambiental) - Universidade Estadual Paulista, Instituto de Geociências e Ciências Exatas, 2009.http://hdl.handle.net/11449/120941000608633sanches_rj_tcc_rcla.pdfAlephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-10-09T06:05:34Zoai:repositorio.unesp.br:11449/120941Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-09T06:05:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel
title Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel
spellingShingle Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel
Sanches, Rafael de Jesus [UNESP]
Microorganismos
Biorremediação
Petróleo
Biodegradation
Biorremediation
Oil
Biodiesel
title_short Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel
title_full Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel
title_fullStr Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel
title_full_unstemmed Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel
title_sort Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel
author Sanches, Rafael de Jesus [UNESP]
author_facet Sanches, Rafael de Jesus [UNESP]
author_role author
dc.contributor.none.fl_str_mv Angelis, Dejanira de Franceschi de [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Sanches, Rafael de Jesus [UNESP]
dc.subject.por.fl_str_mv Microorganismos
Biorremediação
Petróleo
Biodegradation
Biorremediation
Oil
Biodiesel
topic Microorganismos
Biorremediação
Petróleo
Biodegradation
Biorremediation
Oil
Biodiesel
description The oil and biodiesel are oily substances which, if spilled or thrown out in the environment, can cause serious damages. In this context, bioremediation techniques can be used to the recovering of sites degradated by mineral or vegetable oil, besides them, the biodegradation. However, it is necessary that there are microorganism strains used to degradate the contaminant, previously selected for the fast, efficient and minimum impact remediation. The main objective of this study was to isolate microorganism strains coming from different ecological recesses and verify their potentiality on biodegradating oil and biodiesel. Strains of bacteria, fungi and yeasts were isolated from soils contaminated by hydrocarbons (diesel, gasoline, grease) before, by doing striations on culture plates. On the biodegradation tests, it was used the redox indicator, 2,6-dichlorophenol indophenol (DCPIP), then, after the indicador’s discoloration, the absorbance of the suspension obtained was measured. 46 different microorganism strains were isolated, and the oil, when it was used as a carbon resource, it was degradate easier than the biodiesel. The experiments showed that, in a period, the site impacted by oil spills recovers itself by the biodegradation, in aerobic conditions, by the redox activity.
publishDate 2009
dc.date.none.fl_str_mv 2009
2015-03-23T15:28:12Z
2015-03-23T15:28:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SANCHES, Rafael de Jesus. Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel. 2009. vi, 55 f. Trabalho de conclusão de curso (Engenharia Ambiental) - Universidade Estadual Paulista, Instituto de Geociências e Ciências Exatas, 2009.
http://hdl.handle.net/11449/120941
000608633
sanches_rj_tcc_rcla.pdf
identifier_str_mv SANCHES, Rafael de Jesus. Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel. 2009. vi, 55 f. Trabalho de conclusão de curso (Engenharia Ambiental) - Universidade Estadual Paulista, Instituto de Geociências e Ciências Exatas, 2009.
000608633
sanches_rj_tcc_rcla.pdf
url http://hdl.handle.net/11449/120941
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv vi, 55 f.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803649362981552128

Registros relacionados