Total polyphenols from Syzygium cumini (L.) Skeels fruit extract

• Autor(a) principal: Migliato, Ketylin Fernanda [UNESP]
• Data de Publicação: 2009
• Outros Autores: de Carvalho, Elisangela Simoes [UNESP], Silva do Sacramento, Luis Vitor [UNESP], Palazzo de Mello, Joao Carlos, Baby, Andre Rolim, Robles Velasco, Maria Valeria, Salgado, Hérida Regina Nunes [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S1984-82502009000100015; http://hdl.handle.net/11449/7855

A precise, accurate and low cost spectrophotometric method was developed and validated for routine determination of total polyphenols, as pyrogallic acid equivalents, from the percolated and lyophilized extract of Syzygium cumini (L.) Skeels fruits. Validation was assessed experimentally and data were rigorously treated by statistical analysis. Analytical parameters were: linearity, interval (range), precision and recovery/accuracy, limit of detection (LOD, mu g mL(-1)) and limit of quantification (LOQ, mu g mL(-1)). The visible spectrophotometric method presented linearity (r(2) = 0.9979 +/- 0.0010) over the concentration range 0.25-7.5 mu g mL(-1) of standard pyrogallic acid, precision <= 2.918171%, recovery/accuracy ranging from 96.228693 to 107.17701%, LOD = 0.21 mu g mL(-1) and LOQ = 0.64 mu g mL(-1).