Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2017/2180819 http://hdl.handle.net/11449/179098 |
Resumo: | Despite increasing research in type 2 diabetes mellitus (T2D), there are few studies showing the impact of the poor glycemic control on biological processes occurring in T2D. In order to identify potential genes related to poorly/well-controlled patients with T2D, our strategy of investigation included a primary screen by microarray (Human Genome U133) in a small group of individuals followed by an independent validation in a greater group using RT-qPCR. Ninety patients were divided as follows: poorly controlled T2D (G1), well-controlled T2D (G2), and normoglycemic individuals (G3). After using affy package in R, differentially expressed genes (DEGs) were prospected as candidate genes potentially relevant for the glycemic control in T2D patients. After validation by RT-qPCR, the obtained DEGs were as follows - G1 + G2 versus G3: HLA-DQA1, SOS1, and BRCA2; G2 versus G1: ENO2, VAMP2, CCND3, CEBPD, LGALS12, AGBL5, MAP2K5, and PPAP2B; G2 versus G3: HLA-DQB1, MCM4, and SEC13; and G1 versus G3: PPIC. This demonstrated a systemic exacerbation of the gene expression related to immune response in T2D patients. Moreover, genes related to lipid metabolisms and DNA replication/repair were influenced by the glycemic control. In conclusion, this study pointed out candidate genes potentially associated with adequate glycemic control in T2D patients, contributing to the knowledge of how the glycemic control could systemically influence gene expression. |
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Repositório Institucional da UNESP |
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Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitusDespite increasing research in type 2 diabetes mellitus (T2D), there are few studies showing the impact of the poor glycemic control on biological processes occurring in T2D. In order to identify potential genes related to poorly/well-controlled patients with T2D, our strategy of investigation included a primary screen by microarray (Human Genome U133) in a small group of individuals followed by an independent validation in a greater group using RT-qPCR. Ninety patients were divided as follows: poorly controlled T2D (G1), well-controlled T2D (G2), and normoglycemic individuals (G3). After using affy package in R, differentially expressed genes (DEGs) were prospected as candidate genes potentially relevant for the glycemic control in T2D patients. After validation by RT-qPCR, the obtained DEGs were as follows - G1 + G2 versus G3: HLA-DQA1, SOS1, and BRCA2; G2 versus G1: ENO2, VAMP2, CCND3, CEBPD, LGALS12, AGBL5, MAP2K5, and PPAP2B; G2 versus G3: HLA-DQB1, MCM4, and SEC13; and G1 versus G3: PPIC. This demonstrated a systemic exacerbation of the gene expression related to immune response in T2D patients. Moreover, genes related to lipid metabolisms and DNA replication/repair were influenced by the glycemic control. In conclusion, this study pointed out candidate genes potentially associated with adequate glycemic control in T2D patients, contributing to the knowledge of how the glycemic control could systemically influence gene expression.Department of Diagnosis and Surgery School of Dentistry Universidade Estadual Paulista (UNESP)Department of Morphology School of Dentistry Universidade Estadual Paulista (UNESP)Postgraduate Program in Sciences of the University of FrancaDepartment of Genetics Faculty of Medicine of Ribeirão Preto and Department of Biology FFCLRP University of São Paulo (USP)Department of Medical Genetics University of Campinas (UNICAMP)Department of Diagnosis and Surgery School of Dentistry Universidade Estadual Paulista (UNESP)Department of Morphology School of Dentistry Universidade Estadual Paulista (UNESP)Universidade Estadual Paulista (Unesp)Postgraduate Program in Sciences of the University of FrancaUniversidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)Corbi, Sâmia Cruz Tfaile [UNESP]Bastos, Alliny Souza [UNESP]Nepomuceno, Rafael [UNESP]Cirelli, Thamiris [UNESP]Santos, Raquel Alves DosTakahashi, Catarina SatieRocha, Cristiane S.Orrico, Silvana Regina Perez [UNESP]Maurer-Morelli, Claudia V.Scarel-Caminaga, Raquel Mantuaneli [UNESP]2018-12-11T17:33:42Z2018-12-11T17:33:42Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2017/2180819Journal of Diabetes Research, v. 2017.2314-67532314-6745http://hdl.handle.net/11449/17909810.1155/2017/21808192-s2.0-850273003062-s2.0-85027300306.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Diabetes Research1,1161,116info:eu-repo/semantics/openAccess2023-11-27T06:17:06Zoai:repositorio.unesp.br:11449/179098Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-27T06:17:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus |
title |
Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus |
spellingShingle |
Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus Corbi, Sâmia Cruz Tfaile [UNESP] |
title_short |
Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus |
title_full |
Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus |
title_fullStr |
Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus |
title_full_unstemmed |
Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus |
title_sort |
Expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus |
author |
Corbi, Sâmia Cruz Tfaile [UNESP] |
author_facet |
Corbi, Sâmia Cruz Tfaile [UNESP] Bastos, Alliny Souza [UNESP] Nepomuceno, Rafael [UNESP] Cirelli, Thamiris [UNESP] Santos, Raquel Alves Dos Takahashi, Catarina Satie Rocha, Cristiane S. Orrico, Silvana Regina Perez [UNESP] Maurer-Morelli, Claudia V. Scarel-Caminaga, Raquel Mantuaneli [UNESP] |
author_role |
author |
author2 |
Bastos, Alliny Souza [UNESP] Nepomuceno, Rafael [UNESP] Cirelli, Thamiris [UNESP] Santos, Raquel Alves Dos Takahashi, Catarina Satie Rocha, Cristiane S. Orrico, Silvana Regina Perez [UNESP] Maurer-Morelli, Claudia V. Scarel-Caminaga, Raquel Mantuaneli [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Postgraduate Program in Sciences of the University of Franca Universidade de São Paulo (USP) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Corbi, Sâmia Cruz Tfaile [UNESP] Bastos, Alliny Souza [UNESP] Nepomuceno, Rafael [UNESP] Cirelli, Thamiris [UNESP] Santos, Raquel Alves Dos Takahashi, Catarina Satie Rocha, Cristiane S. Orrico, Silvana Regina Perez [UNESP] Maurer-Morelli, Claudia V. Scarel-Caminaga, Raquel Mantuaneli [UNESP] |
description |
Despite increasing research in type 2 diabetes mellitus (T2D), there are few studies showing the impact of the poor glycemic control on biological processes occurring in T2D. In order to identify potential genes related to poorly/well-controlled patients with T2D, our strategy of investigation included a primary screen by microarray (Human Genome U133) in a small group of individuals followed by an independent validation in a greater group using RT-qPCR. Ninety patients were divided as follows: poorly controlled T2D (G1), well-controlled T2D (G2), and normoglycemic individuals (G3). After using affy package in R, differentially expressed genes (DEGs) were prospected as candidate genes potentially relevant for the glycemic control in T2D patients. After validation by RT-qPCR, the obtained DEGs were as follows - G1 + G2 versus G3: HLA-DQA1, SOS1, and BRCA2; G2 versus G1: ENO2, VAMP2, CCND3, CEBPD, LGALS12, AGBL5, MAP2K5, and PPAP2B; G2 versus G3: HLA-DQB1, MCM4, and SEC13; and G1 versus G3: PPIC. This demonstrated a systemic exacerbation of the gene expression related to immune response in T2D patients. Moreover, genes related to lipid metabolisms and DNA replication/repair were influenced by the glycemic control. In conclusion, this study pointed out candidate genes potentially associated with adequate glycemic control in T2D patients, contributing to the knowledge of how the glycemic control could systemically influence gene expression. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:33:42Z 2018-12-11T17:33:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2017/2180819 Journal of Diabetes Research, v. 2017. 2314-6753 2314-6745 http://hdl.handle.net/11449/179098 10.1155/2017/2180819 2-s2.0-85027300306 2-s2.0-85027300306.pdf |
url |
http://dx.doi.org/10.1155/2017/2180819 http://hdl.handle.net/11449/179098 |
identifier_str_mv |
Journal of Diabetes Research, v. 2017. 2314-6753 2314-6745 10.1155/2017/2180819 2-s2.0-85027300306 2-s2.0-85027300306.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Diabetes Research 1,116 1,116 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965091906453504 |