Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.toxicon.2017.10.021 http://hdl.handle.net/11449/170419 |
Resumo: | Microcystins (MCs) are serine/threonine phosphatase inhibitors synthesized by several members of the phylum Cyanobacteria. Mining the draft genome sequence of the nostocalean MC-producing Fischerella sp. strain CENA161 led to the identification of three contigs containing mcy genes. Subsequent PCR and Sanger sequencing allowed the assembling of its complete biosynthetic mcy gene cluster with 55,016 bases in length. The cluster encoding ten genes (mcyA-J) with a central bidirectional promoter was organized in a similar manner as found in other genera of nostocalean cyanobacteria. However, the nucleotide sequence of the mcy gene cluster of Fischerella sp. CENA161 showed significant differences from all the other MC-producing cyanobacterial genera, sharing only 85.2 to 74.1% identities. Potential MC variants produced by Fischerella sp. CENA161 were predicted by the analysis of the adenylation domain binding pockets and further investigated by LC-MS/MS analysis. To our knowledge, this study presents the first complete mcy cluster characterization from a strain of the genus Fischerella, providing new insight into the distribution and evolution of MCs in the phylum Cyanobacteria. |
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Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus FischerellaCyanotoxinsGenome miningNostocalesPhosphatase inhibitorsMicrocystins (MCs) are serine/threonine phosphatase inhibitors synthesized by several members of the phylum Cyanobacteria. Mining the draft genome sequence of the nostocalean MC-producing Fischerella sp. strain CENA161 led to the identification of three contigs containing mcy genes. Subsequent PCR and Sanger sequencing allowed the assembling of its complete biosynthetic mcy gene cluster with 55,016 bases in length. The cluster encoding ten genes (mcyA-J) with a central bidirectional promoter was organized in a similar manner as found in other genera of nostocalean cyanobacteria. However, the nucleotide sequence of the mcy gene cluster of Fischerella sp. CENA161 showed significant differences from all the other MC-producing cyanobacterial genera, sharing only 85.2 to 74.1% identities. Potential MC variants produced by Fischerella sp. CENA161 were predicted by the analysis of the adenylation domain binding pockets and further investigated by LC-MS/MS analysis. To our knowledge, this study presents the first complete mcy cluster characterization from a strain of the genus Fischerella, providing new insight into the distribution and evolution of MCs in the phylum Cyanobacteria.Academy of FinlandFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)University of São Paulo (USP) Center for Nuclear Energy in AgricultureUniversity of Helsinki Department of Food and Environmental Sciences Division of Microbiology and BiotechnologySão Paulo State University (UNESP) College of Agricultural and Veterinary Science Department of TechnologyUniversity of São Paulo (USP) School of Pharmaceutical SciencesSão Paulo State University (UNESP) College of Agricultural and Veterinary Science Department of TechnologyAcademy of Finland: 1273798FAPESP: 2011/08092-6FAPESP: 2013/50425-8FAPESP: 2014/50420-9CNPq: 302599/2016-9CNPq: 310244/2015-3CNPq: 311048/2016-1CAPES: 99999.008344/2014Universidade de São Paulo (USP)Division of Microbiology and BiotechnologyUniversidade Estadual Paulista (Unesp)Heck, KarinaAlvarenga, Danillo O. [UNESP]Shishido, Tania K.Varani, Alessandro M. [UNESP]Dörr, Felipe A.Pinto, ErnaniRouhiainen, LeoJokela, JouniSivonen, KaarinaFiore, Marli F.2018-12-11T16:50:42Z2018-12-11T16:50:42Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article43-50application/pdfhttp://dx.doi.org/10.1016/j.toxicon.2017.10.021Toxicon, v. 141, p. 43-50.1879-31500041-0101http://hdl.handle.net/11449/17041910.1016/j.toxicon.2017.10.0212-s2.0-850350683432-s2.0-85035068343.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicon0,692info:eu-repo/semantics/openAccess2023-11-01T06:14:49Zoai:repositorio.unesp.br:11449/170419Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:39:41.144626Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella |
title |
Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella |
spellingShingle |
Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella Heck, Karina Cyanotoxins Genome mining Nostocales Phosphatase inhibitors |
title_short |
Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella |
title_full |
Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella |
title_fullStr |
Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella |
title_full_unstemmed |
Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella |
title_sort |
Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella |
author |
Heck, Karina |
author_facet |
Heck, Karina Alvarenga, Danillo O. [UNESP] Shishido, Tania K. Varani, Alessandro M. [UNESP] Dörr, Felipe A. Pinto, Ernani Rouhiainen, Leo Jokela, Jouni Sivonen, Kaarina Fiore, Marli F. |
author_role |
author |
author2 |
Alvarenga, Danillo O. [UNESP] Shishido, Tania K. Varani, Alessandro M. [UNESP] Dörr, Felipe A. Pinto, Ernani Rouhiainen, Leo Jokela, Jouni Sivonen, Kaarina Fiore, Marli F. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Division of Microbiology and Biotechnology Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Heck, Karina Alvarenga, Danillo O. [UNESP] Shishido, Tania K. Varani, Alessandro M. [UNESP] Dörr, Felipe A. Pinto, Ernani Rouhiainen, Leo Jokela, Jouni Sivonen, Kaarina Fiore, Marli F. |
dc.subject.por.fl_str_mv |
Cyanotoxins Genome mining Nostocales Phosphatase inhibitors |
topic |
Cyanotoxins Genome mining Nostocales Phosphatase inhibitors |
description |
Microcystins (MCs) are serine/threonine phosphatase inhibitors synthesized by several members of the phylum Cyanobacteria. Mining the draft genome sequence of the nostocalean MC-producing Fischerella sp. strain CENA161 led to the identification of three contigs containing mcy genes. Subsequent PCR and Sanger sequencing allowed the assembling of its complete biosynthetic mcy gene cluster with 55,016 bases in length. The cluster encoding ten genes (mcyA-J) with a central bidirectional promoter was organized in a similar manner as found in other genera of nostocalean cyanobacteria. However, the nucleotide sequence of the mcy gene cluster of Fischerella sp. CENA161 showed significant differences from all the other MC-producing cyanobacterial genera, sharing only 85.2 to 74.1% identities. Potential MC variants produced by Fischerella sp. CENA161 were predicted by the analysis of the adenylation domain binding pockets and further investigated by LC-MS/MS analysis. To our knowledge, this study presents the first complete mcy cluster characterization from a strain of the genus Fischerella, providing new insight into the distribution and evolution of MCs in the phylum Cyanobacteria. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:50:42Z 2018-12-11T16:50:42Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.toxicon.2017.10.021 Toxicon, v. 141, p. 43-50. 1879-3150 0041-0101 http://hdl.handle.net/11449/170419 10.1016/j.toxicon.2017.10.021 2-s2.0-85035068343 2-s2.0-85035068343.pdf |
url |
http://dx.doi.org/10.1016/j.toxicon.2017.10.021 http://hdl.handle.net/11449/170419 |
identifier_str_mv |
Toxicon, v. 141, p. 43-50. 1879-3150 0041-0101 10.1016/j.toxicon.2017.10.021 2-s2.0-85035068343 2-s2.0-85035068343.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicon 0,692 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
43-50 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128684347883520 |