Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng spa |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4067/S0717-95022012000200022 http://hdl.handle.net/11449/227002 |
Resumo: | In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and leptotene was observed, whereas in the adluminal compartment Golgi phase spermatids we observed the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In Group III, 60 days old, it was found that seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. Therefore morphological evolution of germ cell testicular spermatids can be checked and recognized in its four phases: Golgi, cap, acrosome and maturation over the age of the animal. |
id |
UNSP_74a674dc5c39a1bdef489206bfaa95dd |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/227002 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/UniMorphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni miceMouseSpermatogenesisTestesIn order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and leptotene was observed, whereas in the adluminal compartment Golgi phase spermatids we observed the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In Group III, 60 days old, it was found that seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. Therefore morphological evolution of germ cell testicular spermatids can be checked and recognized in its four phases: Golgi, cap, acrosome and maturation over the age of the animal.Universidade Estadual Paulista Departamento de Anatomia, Botucatu - SP - CEP: 18618-970Universidade Estadual Paulista Aluna de Graduação em Zootecnia, Botucatu - SPUniversidade Estadual Paulista Alunos de Graduação em Medicina Veterinária, Botucatu - SPUniversidade Estadual Paulista Departamento de Anatomia, Botucatu - SP - CEP: 18618-970Universidade Estadual Paulista Aluna de Graduação em Zootecnia, Botucatu - SPUniversidade Estadual Paulista Alunos de Graduação em Medicina Veterinária, Botucatu - SPUniversidade Estadual Paulista (UNESP)Martins, M. R.F.B. [UNESP]Silva, P. J.R. [UNESP]Martins, B. B. [UNESP]Zoca, S. M. [UNESP]Freitas, M. A. [UNESP]Batista, T. C. [UNESP]2022-04-29T05:36:42Z2022-04-29T05:36:42Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article493-498http://dx.doi.org/10.4067/S0717-95022012000200022International Journal of Morphology, v. 30, n. 2, p. 493-498, 2012.0717-95020717-9367http://hdl.handle.net/11449/22700210.4067/S0717-950220120002000222-s2.0-84867514778Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengspaInternational Journal of Morphologyinfo:eu-repo/semantics/openAccess2022-04-29T05:36:42Zoai:repositorio.unesp.br:11449/227002Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:40:11.128667Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice |
title |
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni |
spellingShingle |
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni Martins, M. R.F.B. [UNESP] Mouse Spermatogenesis Testes |
title_short |
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni |
title_full |
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni |
title_fullStr |
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni |
title_full_unstemmed |
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni |
title_sort |
Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni |
author |
Martins, M. R.F.B. [UNESP] |
author_facet |
Martins, M. R.F.B. [UNESP] Silva, P. J.R. [UNESP] Martins, B. B. [UNESP] Zoca, S. M. [UNESP] Freitas, M. A. [UNESP] Batista, T. C. [UNESP] |
author_role |
author |
author2 |
Silva, P. J.R. [UNESP] Martins, B. B. [UNESP] Zoca, S. M. [UNESP] Freitas, M. A. [UNESP] Batista, T. C. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Martins, M. R.F.B. [UNESP] Silva, P. J.R. [UNESP] Martins, B. B. [UNESP] Zoca, S. M. [UNESP] Freitas, M. A. [UNESP] Batista, T. C. [UNESP] |
dc.subject.por.fl_str_mv |
Mouse Spermatogenesis Testes |
topic |
Mouse Spermatogenesis Testes |
description |
In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and leptotene was observed, whereas in the adluminal compartment Golgi phase spermatids we observed the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In Group III, 60 days old, it was found that seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. Therefore morphological evolution of germ cell testicular spermatids can be checked and recognized in its four phases: Golgi, cap, acrosome and maturation over the age of the animal. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2022-04-29T05:36:42Z 2022-04-29T05:36:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4067/S0717-95022012000200022 International Journal of Morphology, v. 30, n. 2, p. 493-498, 2012. 0717-9502 0717-9367 http://hdl.handle.net/11449/227002 10.4067/S0717-95022012000200022 2-s2.0-84867514778 |
url |
http://dx.doi.org/10.4067/S0717-95022012000200022 http://hdl.handle.net/11449/227002 |
identifier_str_mv |
International Journal of Morphology, v. 30, n. 2, p. 493-498, 2012. 0717-9502 0717-9367 10.4067/S0717-95022012000200022 2-s2.0-84867514778 |
dc.language.iso.fl_str_mv |
eng spa |
language |
eng spa |
dc.relation.none.fl_str_mv |
International Journal of Morphology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
493-498 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129232824434688 |