Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegypti
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4136/ambi-agua.1993 http://hdl.handle.net/11449/169660 |
Resumo: | The Aedes aegypti mosquito is a vector of diseases such as dengue, yellow fever, chikungunya and zika, which has great relevance to public health. In urban areas, ultra-low volume (ULV) malathion fogging is used for the control of adult mosquitoes. Spraying with the insecticide can contaminate soil and water. The goal of this study was to adjust and validate an analytical method for the determination of malathion in high-performance liquid chromatography (HPLC) and determine the pesticide extraction method of water and soil matrices. The mobile phase used an isocratic mixture of acetonitrile and water (68:32), at a flow rate of 1 mL min-1, an injection volume of 20 μL, and a UV at 210 nm. After fortification, the analyte was extracted with acetonitrile (ACN) by mechanical agitation of the soil samples, and by evaporation of the water samples, pre-concentrated, re-dissolved in ACN, and the recovery was calculated. The method is simple, specific and suitable for analysis of malathion with linearity between concentrations of 0.050 and 1.00 μg mL-1 (R2 = 0.999) and detection limits of 0,017 μg mL-1 and quantification of 0.051 μg mL-1. The average recovery of malathion from water was 97%, and from soil, 96%, being the method exact and precise (coefficient of variation <8.8%). The set method can be safely used for the quantification of the insecticide in water and soil matrices. |
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Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegyptiAnalytical method of validation and extraction of malathion in water and soil after fogging against Aedes aegyptiInsecticideLiquid chromatographyPublic healthThe Aedes aegypti mosquito is a vector of diseases such as dengue, yellow fever, chikungunya and zika, which has great relevance to public health. In urban areas, ultra-low volume (ULV) malathion fogging is used for the control of adult mosquitoes. Spraying with the insecticide can contaminate soil and water. The goal of this study was to adjust and validate an analytical method for the determination of malathion in high-performance liquid chromatography (HPLC) and determine the pesticide extraction method of water and soil matrices. The mobile phase used an isocratic mixture of acetonitrile and water (68:32), at a flow rate of 1 mL min-1, an injection volume of 20 μL, and a UV at 210 nm. After fortification, the analyte was extracted with acetonitrile (ACN) by mechanical agitation of the soil samples, and by evaporation of the water samples, pre-concentrated, re-dissolved in ACN, and the recovery was calculated. The method is simple, specific and suitable for analysis of malathion with linearity between concentrations of 0.050 and 1.00 μg mL-1 (R2 = 0.999) and detection limits of 0,017 μg mL-1 and quantification of 0.051 μg mL-1. The average recovery of malathion from water was 97%, and from soil, 96%, being the method exact and precise (coefficient of variation <8.8%). The set method can be safely used for the quantification of the insecticide in water and soil matrices.Departamento de Saúde Ambiental Universidade de São Paulo (USP)Departamento de Fitossanidade Universidade Estadual Paulista (UNESP)Universidade Federal de Itajubá (UNIFEI)Departamento de Fitossanidade Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Universidade Federal de Itajubá (UNIFEI)Coleone, Ana Carla [UNESP]da Silva, Flavio SoaresMachado, Angela Aparecida [UNESP]Neto, Joaquim Gonçalves Machado [UNESP]Paganini, Wanderley da Silva2018-12-11T16:47:03Z2018-12-11T16:47:03Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article426-434application/pdfhttp://dx.doi.org/10.4136/ambi-agua.1993Revista Ambiente e Agua, v. 12, n. 3, p. 426-434, 2017.1980-993Xhttp://hdl.handle.net/11449/16966010.4136/ambi-agua.1993S1980-993X20170003004262-s2.0-85018441157S1980-993X2017000300426.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Ambiente e Aguainfo:eu-repo/semantics/openAccess2023-11-05T06:09:24Zoai:repositorio.unesp.br:11449/169660Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:58:49.315412Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegypti Analytical method of validation and extraction of malathion in water and soil after fogging against Aedes aegypti |
title |
Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegypti |
spellingShingle |
Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegypti Coleone, Ana Carla [UNESP] Insecticide Liquid chromatography Public health |
title_short |
Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegypti |
title_full |
Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegypti |
title_fullStr |
Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegypti |
title_full_unstemmed |
Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegypti |
title_sort |
Validação de método analítico e de extração do malation em água e solo após nebulização de combate ao Aedes aegypti |
author |
Coleone, Ana Carla [UNESP] |
author_facet |
Coleone, Ana Carla [UNESP] da Silva, Flavio Soares Machado, Angela Aparecida [UNESP] Neto, Joaquim Gonçalves Machado [UNESP] Paganini, Wanderley da Silva |
author_role |
author |
author2 |
da Silva, Flavio Soares Machado, Angela Aparecida [UNESP] Neto, Joaquim Gonçalves Machado [UNESP] Paganini, Wanderley da Silva |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Universidade Federal de Itajubá (UNIFEI) |
dc.contributor.author.fl_str_mv |
Coleone, Ana Carla [UNESP] da Silva, Flavio Soares Machado, Angela Aparecida [UNESP] Neto, Joaquim Gonçalves Machado [UNESP] Paganini, Wanderley da Silva |
dc.subject.por.fl_str_mv |
Insecticide Liquid chromatography Public health |
topic |
Insecticide Liquid chromatography Public health |
description |
The Aedes aegypti mosquito is a vector of diseases such as dengue, yellow fever, chikungunya and zika, which has great relevance to public health. In urban areas, ultra-low volume (ULV) malathion fogging is used for the control of adult mosquitoes. Spraying with the insecticide can contaminate soil and water. The goal of this study was to adjust and validate an analytical method for the determination of malathion in high-performance liquid chromatography (HPLC) and determine the pesticide extraction method of water and soil matrices. The mobile phase used an isocratic mixture of acetonitrile and water (68:32), at a flow rate of 1 mL min-1, an injection volume of 20 μL, and a UV at 210 nm. After fortification, the analyte was extracted with acetonitrile (ACN) by mechanical agitation of the soil samples, and by evaporation of the water samples, pre-concentrated, re-dissolved in ACN, and the recovery was calculated. The method is simple, specific and suitable for analysis of malathion with linearity between concentrations of 0.050 and 1.00 μg mL-1 (R2 = 0.999) and detection limits of 0,017 μg mL-1 and quantification of 0.051 μg mL-1. The average recovery of malathion from water was 97%, and from soil, 96%, being the method exact and precise (coefficient of variation <8.8%). The set method can be safely used for the quantification of the insecticide in water and soil matrices. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T16:47:03Z 2018-12-11T16:47:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4136/ambi-agua.1993 Revista Ambiente e Agua, v. 12, n. 3, p. 426-434, 2017. 1980-993X http://hdl.handle.net/11449/169660 10.4136/ambi-agua.1993 S1980-993X2017000300426 2-s2.0-85018441157 S1980-993X2017000300426.pdf |
url |
http://dx.doi.org/10.4136/ambi-agua.1993 http://hdl.handle.net/11449/169660 |
identifier_str_mv |
Revista Ambiente e Agua, v. 12, n. 3, p. 426-434, 2017. 1980-993X 10.4136/ambi-agua.1993 S1980-993X2017000300426 2-s2.0-85018441157 S1980-993X2017000300426.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Ambiente e Agua |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
426-434 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128728422678528 |