Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis

Detalhes bibliográficos
Autor(a) principal: Sani, Alessandra Aguirra [UNESP]
Data de Publicação: 2022
Outros Autores: Pereira, Ana Flávia Marques [UNESP], Furlanetto, Alessandra [UNESP], de Sousa, Débora Silva Marques [UNESP], Zapata, Tatiane Baptista [UNESP], Rall, Vera Lucia Mores [UNESP], Júnior, Ary Fernandes [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-9199-JVATITD-2022-0025
http://hdl.handle.net/11449/249442
Resumo: Background: Natural products represent important sources of antimicrobial compounds. Propolis and compounds from essential oils comprise good examples of such substances because of their inhibitory effects on bacterial spores, including bee pathogens. Methods: Ethanol extracts of propolis (EEP) from Apis mellifera were prepared using different methods: double ultrasonication, double maceration and maceration associated with ultrasonication. Together with the antimicrobial peptides nisin and melittin, and compounds present in the essential oils of clove (Syzygium aromaticum) and cinnamon (Cinnamomum zeylanicum), assays were carried out on one Bacillus subtilis isolate and Paenibacillus alvei (ATCC 6344) against vegetative and sporulated forms, using the resazurin microtiter assay. Synergism with all the antimicrobials in association with tetracycline was verified by the time-kill curve method. Potassium and phosphate efflux, release of proteins and nucleic acids were investigated. Results: EEPs showed the same MIC, 156.25 µg/mL against B. subtilis and 78.12 µg/mL against P. alvei. The peptides showed better activities against B. subtilis (MIC of 12 µg/ mL for melittin and 37.50 µg/mL for nisin). Antimicrobials showed similar inhibitory effects, but cinnamaldehyde (39.06 µg/mL) showed the best action against P. alvei. Melittin and nisin showed the greatest capacity to reduce spores, regarding B. subtilis there was a 100% reduction at 6.25 and 0.78 µg/mL, respectively. Concerning P. alvei, the reduction was 93 and 98% at concentrations of 80 µg/mL of melittin and 15 µg/ mL of nisin. EEPs showed the highest effects on the protein release against B. subtilis and P. alvei. Nucleic acid release, phosphate and potassium efflux assays indicated bacterial cell membrane damage. Synergism between antimicrobials and tetracycline was demonstrated against both bacteria. Conclusion: All antimicrobials tested showed antibacterial activities against vegetative and sporulated forms of P. alvei and B. subtilis, especially nisin and melittin. Synergism with tetracycline and damage on bacterial cell membrane also occurred.
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spelling Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilisApis melliferaCinnamaldehydeEugenolMelittinNisinSporicidal activitySynergismTetracyclineBackground: Natural products represent important sources of antimicrobial compounds. Propolis and compounds from essential oils comprise good examples of such substances because of their inhibitory effects on bacterial spores, including bee pathogens. Methods: Ethanol extracts of propolis (EEP) from Apis mellifera were prepared using different methods: double ultrasonication, double maceration and maceration associated with ultrasonication. Together with the antimicrobial peptides nisin and melittin, and compounds present in the essential oils of clove (Syzygium aromaticum) and cinnamon (Cinnamomum zeylanicum), assays were carried out on one Bacillus subtilis isolate and Paenibacillus alvei (ATCC 6344) against vegetative and sporulated forms, using the resazurin microtiter assay. Synergism with all the antimicrobials in association with tetracycline was verified by the time-kill curve method. Potassium and phosphate efflux, release of proteins and nucleic acids were investigated. Results: EEPs showed the same MIC, 156.25 µg/mL against B. subtilis and 78.12 µg/mL against P. alvei. The peptides showed better activities against B. subtilis (MIC of 12 µg/ mL for melittin and 37.50 µg/mL for nisin). Antimicrobials showed similar inhibitory effects, but cinnamaldehyde (39.06 µg/mL) showed the best action against P. alvei. Melittin and nisin showed the greatest capacity to reduce spores, regarding B. subtilis there was a 100% reduction at 6.25 and 0.78 µg/mL, respectively. Concerning P. alvei, the reduction was 93 and 98% at concentrations of 80 µg/mL of melittin and 15 µg/ mL of nisin. EEPs showed the highest effects on the protein release against B. subtilis and P. alvei. Nucleic acid release, phosphate and potassium efflux assays indicated bacterial cell membrane damage. Synergism between antimicrobials and tetracycline was demonstrated against both bacteria. Conclusion: All antimicrobials tested showed antibacterial activities against vegetative and sporulated forms of P. alvei and B. subtilis, especially nisin and melittin. Synergism with tetracycline and damage on bacterial cell membrane also occurred.Department of Chemical and Biological Sciences Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP), SPDepartment of Chemical and Biological Sciences Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)Sani, Alessandra Aguirra [UNESP]Pereira, Ana Flávia Marques [UNESP]Furlanetto, Alessandra [UNESP]de Sousa, Débora Silva Marques [UNESP]Zapata, Tatiane Baptista [UNESP]Rall, Vera Lucia Mores [UNESP]Júnior, Ary Fernandes [UNESP]2023-07-29T15:41:24Z2023-07-29T15:41:24Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/1678-9199-JVATITD-2022-0025Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 28.1678-91991678-9180http://hdl.handle.net/11449/24944210.1590/1678-9199-JVATITD-2022-00252-s2.0-85143397887Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2023-07-29T15:41:24Zoai:repositorio.unesp.br:11449/249442Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:02:53.061209Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis
title Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis
spellingShingle Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis
Sani, Alessandra Aguirra [UNESP]
Apis mellifera
Cinnamaldehyde
Eugenol
Melittin
Nisin
Sporicidal activity
Synergism
Tetracycline
title_short Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis
title_full Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis
title_fullStr Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis
title_full_unstemmed Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis
title_sort Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis
author Sani, Alessandra Aguirra [UNESP]
author_facet Sani, Alessandra Aguirra [UNESP]
Pereira, Ana Flávia Marques [UNESP]
Furlanetto, Alessandra [UNESP]
de Sousa, Débora Silva Marques [UNESP]
Zapata, Tatiane Baptista [UNESP]
Rall, Vera Lucia Mores [UNESP]
Júnior, Ary Fernandes [UNESP]
author_role author
author2 Pereira, Ana Flávia Marques [UNESP]
Furlanetto, Alessandra [UNESP]
de Sousa, Débora Silva Marques [UNESP]
Zapata, Tatiane Baptista [UNESP]
Rall, Vera Lucia Mores [UNESP]
Júnior, Ary Fernandes [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Sani, Alessandra Aguirra [UNESP]
Pereira, Ana Flávia Marques [UNESP]
Furlanetto, Alessandra [UNESP]
de Sousa, Débora Silva Marques [UNESP]
Zapata, Tatiane Baptista [UNESP]
Rall, Vera Lucia Mores [UNESP]
Júnior, Ary Fernandes [UNESP]
dc.subject.por.fl_str_mv Apis mellifera
Cinnamaldehyde
Eugenol
Melittin
Nisin
Sporicidal activity
Synergism
Tetracycline
topic Apis mellifera
Cinnamaldehyde
Eugenol
Melittin
Nisin
Sporicidal activity
Synergism
Tetracycline
description Background: Natural products represent important sources of antimicrobial compounds. Propolis and compounds from essential oils comprise good examples of such substances because of their inhibitory effects on bacterial spores, including bee pathogens. Methods: Ethanol extracts of propolis (EEP) from Apis mellifera were prepared using different methods: double ultrasonication, double maceration and maceration associated with ultrasonication. Together with the antimicrobial peptides nisin and melittin, and compounds present in the essential oils of clove (Syzygium aromaticum) and cinnamon (Cinnamomum zeylanicum), assays were carried out on one Bacillus subtilis isolate and Paenibacillus alvei (ATCC 6344) against vegetative and sporulated forms, using the resazurin microtiter assay. Synergism with all the antimicrobials in association with tetracycline was verified by the time-kill curve method. Potassium and phosphate efflux, release of proteins and nucleic acids were investigated. Results: EEPs showed the same MIC, 156.25 µg/mL against B. subtilis and 78.12 µg/mL against P. alvei. The peptides showed better activities against B. subtilis (MIC of 12 µg/ mL for melittin and 37.50 µg/mL for nisin). Antimicrobials showed similar inhibitory effects, but cinnamaldehyde (39.06 µg/mL) showed the best action against P. alvei. Melittin and nisin showed the greatest capacity to reduce spores, regarding B. subtilis there was a 100% reduction at 6.25 and 0.78 µg/mL, respectively. Concerning P. alvei, the reduction was 93 and 98% at concentrations of 80 µg/mL of melittin and 15 µg/ mL of nisin. EEPs showed the highest effects on the protein release against B. subtilis and P. alvei. Nucleic acid release, phosphate and potassium efflux assays indicated bacterial cell membrane damage. Synergism between antimicrobials and tetracycline was demonstrated against both bacteria. Conclusion: All antimicrobials tested showed antibacterial activities against vegetative and sporulated forms of P. alvei and B. subtilis, especially nisin and melittin. Synergism with tetracycline and damage on bacterial cell membrane also occurred.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-07-29T15:41:24Z
2023-07-29T15:41:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-9199-JVATITD-2022-0025
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 28.
1678-9199
1678-9180
http://hdl.handle.net/11449/249442
10.1590/1678-9199-JVATITD-2022-0025
2-s2.0-85143397887
url http://dx.doi.org/10.1590/1678-9199-JVATITD-2022-0025
http://hdl.handle.net/11449/249442
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 28.
1678-9199
1678-9180
10.1590/1678-9199-JVATITD-2022-0025
2-s2.0-85143397887
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128599402741760