Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas

Detalhes bibliográficos
Autor(a) principal: Ferreira, Marina Neves [UNESP]
Data de Publicação: 2017
Outros Autores: Bonini-Domingos, Claudia Regina [UNESP], Fonseca Estevão, Isabeth [UNESP], De Castro Lobo, Clarice Lopes, Souza Carrocini, Gisele Cristina [UNESP], Silveira-Carvalho, Aparecida Perpétuo, Ricci, Octávio, De Mattos, Luiz Carlos, Brandão De Mattos, Cinara Cássia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s13104-017-2535-7
http://hdl.handle.net/11449/175061
Resumo: Background In Brazil, there have been no previous studies of Toxoplasma gondii infection in sickle cell anemia patients and carriers of severe forms of beta-thalassemia. This study evaluated T. gondii infection in patients with beta-hemoglobinopathies. Methods A total of 158 samples, 77 (48.7%) men and 81 (51.3%) women, were evaluated. Three groups were formed: G1 (85 patients with sickle cell disease); G2 (11 patients with homozygous beta-thalassemia; G3 (62 patients with heterozygous beta-thalassemia). ELISA was employed to identify anti-T. gondii IgM and IgG antibodies, and molecular analysis was performed to determine beta-hemoglobin mutations. Fisher's exact test was used to compare frequencies of anti-T. gondii IgM and IgG antibodies in respect to gender and age. Results Anti-T. gondii IgG antibodies were found in 43.5% of individuals in G1, 18.1% in G2 and 50% in G3. All samples from G1 and G2 were seronegative for anti-T. gondii IgM antibodies, but 3.2% from G3 were seropositive. Considering anti-T. gondii IgG antibodies, no statistical significant differences were found between these groups nor in seroprevalence between genders within each group. Despite this, comparisons of the mean ages between G1, G2 and G3 were statistically significant (G2 vs. G1: p value = 0.0001; G3 vs. G1: p-value <0.0001; G3 vs. G2: p-value = 0.0001). Conclusion A comparison by age of patients with sickle cell anemia showed a trend of lower risk of infection among younger individuals. Therefore, this study demonstrates that T. gondii infection occurs in patients with beta-thalassemia and sickle cell anemia in Brazil as seen by the presence of anti-T. gondii IgM and IgG antibodies.
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spelling Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the AmericasBeta hemoglobinopathiesBeta-thalassemiaSickle cell diseaseToxoplasma gondiiToxoplasmosisBackground In Brazil, there have been no previous studies of Toxoplasma gondii infection in sickle cell anemia patients and carriers of severe forms of beta-thalassemia. This study evaluated T. gondii infection in patients with beta-hemoglobinopathies. Methods A total of 158 samples, 77 (48.7%) men and 81 (51.3%) women, were evaluated. Three groups were formed: G1 (85 patients with sickle cell disease); G2 (11 patients with homozygous beta-thalassemia; G3 (62 patients with heterozygous beta-thalassemia). ELISA was employed to identify anti-T. gondii IgM and IgG antibodies, and molecular analysis was performed to determine beta-hemoglobin mutations. Fisher's exact test was used to compare frequencies of anti-T. gondii IgM and IgG antibodies in respect to gender and age. Results Anti-T. gondii IgG antibodies were found in 43.5% of individuals in G1, 18.1% in G2 and 50% in G3. All samples from G1 and G2 were seronegative for anti-T. gondii IgM antibodies, but 3.2% from G3 were seropositive. Considering anti-T. gondii IgG antibodies, no statistical significant differences were found between these groups nor in seroprevalence between genders within each group. Despite this, comparisons of the mean ages between G1, G2 and G3 were statistically significant (G2 vs. G1: p value = 0.0001; G3 vs. G1: p-value <0.0001; G3 vs. G2: p-value = 0.0001). Conclusion A comparison by age of patients with sickle cell anemia showed a trend of lower risk of infection among younger individuals. Therefore, this study demonstrates that T. gondii infection occurs in patients with beta-thalassemia and sickle cell anemia in Brazil as seen by the presence of anti-T. gondii IgM and IgG antibodies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biology Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Letras e Ciências Exatas-IBILCE-UNESPImmunogenetics Laboratory Department of Molecular Biology Faculdade de Medicina de São José Do Rio Preto-FAMERP, Avenida Brigadeiro Faria Lima, 5416FAMERP Toxoplasma Research Group Faculdade de Medicina de São José Do Rio PretoClinical Hematology Division Instituto de Hematologia Arthur de Siqueira Cavalcanti-HEMORIODepartment of Medicine Faculdade de Medicina de São José Do Rio Preto-FAMERPRegional Blood Center Hemocentro de São José Do Rio Preto Fundação Faculdade Regional de Medicina-FUNFARMEFaculdade de Medicina Veterinária e Zootecnia Universidade de São PauloDepartment of Biology Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Letras e Ciências Exatas-IBILCE-UNESPFAPESP: 2011/15570-1FAPESP: 2012/07716-9FAPESP: 2014/01706-7FAPESP: 2015/04677-0Universidade Estadual Paulista (Unesp)Faculdade de Medicina de São José Do Rio Preto-FAMERPFaculdade de Medicina de São José Do Rio PretoInstituto de Hematologia Arthur de Siqueira Cavalcanti-HEMORIOFundação Faculdade Regional de Medicina-FUNFARMEUniversidade de São Paulo (USP)Ferreira, Marina Neves [UNESP]Bonini-Domingos, Claudia Regina [UNESP]Fonseca Estevão, Isabeth [UNESP]De Castro Lobo, Clarice LopesSouza Carrocini, Gisele Cristina [UNESP]Silveira-Carvalho, Aparecida PerpétuoRicci, OctávioDe Mattos, Luiz CarlosBrandão De Mattos, Cinara Cássia2018-12-11T17:14:03Z2018-12-11T17:14:03Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s13104-017-2535-7BMC Research Notes, v. 1, n. 1, 2017.1756-0500http://hdl.handle.net/11449/17506110.1186/s13104-017-2535-72-s2.0-850278421802-s2.0-85027842180.pdf32794280661767190000-0002-4603-9467Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Research Notes0,691info:eu-repo/semantics/openAccess2023-11-25T06:19:05Zoai:repositorio.unesp.br:11449/175061Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:43:54.428232Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas
title Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas
spellingShingle Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas
Ferreira, Marina Neves [UNESP]
Beta hemoglobinopathies
Beta-thalassemia
Sickle cell disease
Toxoplasma gondii
Toxoplasmosis
title_short Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas
title_full Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas
title_fullStr Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas
title_full_unstemmed Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas
title_sort Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas
author Ferreira, Marina Neves [UNESP]
author_facet Ferreira, Marina Neves [UNESP]
Bonini-Domingos, Claudia Regina [UNESP]
Fonseca Estevão, Isabeth [UNESP]
De Castro Lobo, Clarice Lopes
Souza Carrocini, Gisele Cristina [UNESP]
Silveira-Carvalho, Aparecida Perpétuo
Ricci, Octávio
De Mattos, Luiz Carlos
Brandão De Mattos, Cinara Cássia
author_role author
author2 Bonini-Domingos, Claudia Regina [UNESP]
Fonseca Estevão, Isabeth [UNESP]
De Castro Lobo, Clarice Lopes
Souza Carrocini, Gisele Cristina [UNESP]
Silveira-Carvalho, Aparecida Perpétuo
Ricci, Octávio
De Mattos, Luiz Carlos
Brandão De Mattos, Cinara Cássia
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Faculdade de Medicina de São José Do Rio Preto-FAMERP
Faculdade de Medicina de São José Do Rio Preto
Instituto de Hematologia Arthur de Siqueira Cavalcanti-HEMORIO
Fundação Faculdade Regional de Medicina-FUNFARME
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Ferreira, Marina Neves [UNESP]
Bonini-Domingos, Claudia Regina [UNESP]
Fonseca Estevão, Isabeth [UNESP]
De Castro Lobo, Clarice Lopes
Souza Carrocini, Gisele Cristina [UNESP]
Silveira-Carvalho, Aparecida Perpétuo
Ricci, Octávio
De Mattos, Luiz Carlos
Brandão De Mattos, Cinara Cássia
dc.subject.por.fl_str_mv Beta hemoglobinopathies
Beta-thalassemia
Sickle cell disease
Toxoplasma gondii
Toxoplasmosis
topic Beta hemoglobinopathies
Beta-thalassemia
Sickle cell disease
Toxoplasma gondii
Toxoplasmosis
description Background In Brazil, there have been no previous studies of Toxoplasma gondii infection in sickle cell anemia patients and carriers of severe forms of beta-thalassemia. This study evaluated T. gondii infection in patients with beta-hemoglobinopathies. Methods A total of 158 samples, 77 (48.7%) men and 81 (51.3%) women, were evaluated. Three groups were formed: G1 (85 patients with sickle cell disease); G2 (11 patients with homozygous beta-thalassemia; G3 (62 patients with heterozygous beta-thalassemia). ELISA was employed to identify anti-T. gondii IgM and IgG antibodies, and molecular analysis was performed to determine beta-hemoglobin mutations. Fisher's exact test was used to compare frequencies of anti-T. gondii IgM and IgG antibodies in respect to gender and age. Results Anti-T. gondii IgG antibodies were found in 43.5% of individuals in G1, 18.1% in G2 and 50% in G3. All samples from G1 and G2 were seronegative for anti-T. gondii IgM antibodies, but 3.2% from G3 were seropositive. Considering anti-T. gondii IgG antibodies, no statistical significant differences were found between these groups nor in seroprevalence between genders within each group. Despite this, comparisons of the mean ages between G1, G2 and G3 were statistically significant (G2 vs. G1: p value = 0.0001; G3 vs. G1: p-value <0.0001; G3 vs. G2: p-value = 0.0001). Conclusion A comparison by age of patients with sickle cell anemia showed a trend of lower risk of infection among younger individuals. Therefore, this study demonstrates that T. gondii infection occurs in patients with beta-thalassemia and sickle cell anemia in Brazil as seen by the presence of anti-T. gondii IgM and IgG antibodies.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
2018-12-11T17:14:03Z
2018-12-11T17:14:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s13104-017-2535-7
BMC Research Notes, v. 1, n. 1, 2017.
1756-0500
http://hdl.handle.net/11449/175061
10.1186/s13104-017-2535-7
2-s2.0-85027842180
2-s2.0-85027842180.pdf
3279428066176719
0000-0002-4603-9467
url http://dx.doi.org/10.1186/s13104-017-2535-7
http://hdl.handle.net/11449/175061
identifier_str_mv BMC Research Notes, v. 1, n. 1, 2017.
1756-0500
10.1186/s13104-017-2535-7
2-s2.0-85027842180
2-s2.0-85027842180.pdf
3279428066176719
0000-0002-4603-9467
dc.language.iso.fl_str_mv eng
language eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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