Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s13104-017-2535-7 http://hdl.handle.net/11449/175061 |
Resumo: | Background In Brazil, there have been no previous studies of Toxoplasma gondii infection in sickle cell anemia patients and carriers of severe forms of beta-thalassemia. This study evaluated T. gondii infection in patients with beta-hemoglobinopathies. Methods A total of 158 samples, 77 (48.7%) men and 81 (51.3%) women, were evaluated. Three groups were formed: G1 (85 patients with sickle cell disease); G2 (11 patients with homozygous beta-thalassemia; G3 (62 patients with heterozygous beta-thalassemia). ELISA was employed to identify anti-T. gondii IgM and IgG antibodies, and molecular analysis was performed to determine beta-hemoglobin mutations. Fisher's exact test was used to compare frequencies of anti-T. gondii IgM and IgG antibodies in respect to gender and age. Results Anti-T. gondii IgG antibodies were found in 43.5% of individuals in G1, 18.1% in G2 and 50% in G3. All samples from G1 and G2 were seronegative for anti-T. gondii IgM antibodies, but 3.2% from G3 were seropositive. Considering anti-T. gondii IgG antibodies, no statistical significant differences were found between these groups nor in seroprevalence between genders within each group. Despite this, comparisons of the mean ages between G1, G2 and G3 were statistically significant (G2 vs. G1: p value = 0.0001; G3 vs. G1: p-value <0.0001; G3 vs. G2: p-value = 0.0001). Conclusion A comparison by age of patients with sickle cell anemia showed a trend of lower risk of infection among younger individuals. Therefore, this study demonstrates that T. gondii infection occurs in patients with beta-thalassemia and sickle cell anemia in Brazil as seen by the presence of anti-T. gondii IgM and IgG antibodies. |
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Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the AmericasBeta hemoglobinopathiesBeta-thalassemiaSickle cell diseaseToxoplasma gondiiToxoplasmosisBackground In Brazil, there have been no previous studies of Toxoplasma gondii infection in sickle cell anemia patients and carriers of severe forms of beta-thalassemia. This study evaluated T. gondii infection in patients with beta-hemoglobinopathies. Methods A total of 158 samples, 77 (48.7%) men and 81 (51.3%) women, were evaluated. Three groups were formed: G1 (85 patients with sickle cell disease); G2 (11 patients with homozygous beta-thalassemia; G3 (62 patients with heterozygous beta-thalassemia). ELISA was employed to identify anti-T. gondii IgM and IgG antibodies, and molecular analysis was performed to determine beta-hemoglobin mutations. Fisher's exact test was used to compare frequencies of anti-T. gondii IgM and IgG antibodies in respect to gender and age. Results Anti-T. gondii IgG antibodies were found in 43.5% of individuals in G1, 18.1% in G2 and 50% in G3. All samples from G1 and G2 were seronegative for anti-T. gondii IgM antibodies, but 3.2% from G3 were seropositive. Considering anti-T. gondii IgG antibodies, no statistical significant differences were found between these groups nor in seroprevalence between genders within each group. Despite this, comparisons of the mean ages between G1, G2 and G3 were statistically significant (G2 vs. G1: p value = 0.0001; G3 vs. G1: p-value <0.0001; G3 vs. G2: p-value = 0.0001). Conclusion A comparison by age of patients with sickle cell anemia showed a trend of lower risk of infection among younger individuals. Therefore, this study demonstrates that T. gondii infection occurs in patients with beta-thalassemia and sickle cell anemia in Brazil as seen by the presence of anti-T. gondii IgM and IgG antibodies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biology Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Letras e Ciências Exatas-IBILCE-UNESPImmunogenetics Laboratory Department of Molecular Biology Faculdade de Medicina de São José Do Rio Preto-FAMERP, Avenida Brigadeiro Faria Lima, 5416FAMERP Toxoplasma Research Group Faculdade de Medicina de São José Do Rio PretoClinical Hematology Division Instituto de Hematologia Arthur de Siqueira Cavalcanti-HEMORIODepartment of Medicine Faculdade de Medicina de São José Do Rio Preto-FAMERPRegional Blood Center Hemocentro de São José Do Rio Preto Fundação Faculdade Regional de Medicina-FUNFARMEFaculdade de Medicina Veterinária e Zootecnia Universidade de São PauloDepartment of Biology Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Letras e Ciências Exatas-IBILCE-UNESPFAPESP: 2011/15570-1FAPESP: 2012/07716-9FAPESP: 2014/01706-7FAPESP: 2015/04677-0Universidade Estadual Paulista (Unesp)Faculdade de Medicina de São José Do Rio Preto-FAMERPFaculdade de Medicina de São José Do Rio PretoInstituto de Hematologia Arthur de Siqueira Cavalcanti-HEMORIOFundação Faculdade Regional de Medicina-FUNFARMEUniversidade de São Paulo (USP)Ferreira, Marina Neves [UNESP]Bonini-Domingos, Claudia Regina [UNESP]Fonseca Estevão, Isabeth [UNESP]De Castro Lobo, Clarice LopesSouza Carrocini, Gisele Cristina [UNESP]Silveira-Carvalho, Aparecida PerpétuoRicci, OctávioDe Mattos, Luiz CarlosBrandão De Mattos, Cinara Cássia2018-12-11T17:14:03Z2018-12-11T17:14:03Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s13104-017-2535-7BMC Research Notes, v. 1, n. 1, 2017.1756-0500http://hdl.handle.net/11449/17506110.1186/s13104-017-2535-72-s2.0-850278421802-s2.0-85027842180.pdf32794280661767190000-0002-4603-9467Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Research Notes0,691info:eu-repo/semantics/openAccess2023-11-25T06:19:05Zoai:repositorio.unesp.br:11449/175061Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:43:54.428232Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas |
title |
Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas |
spellingShingle |
Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas Ferreira, Marina Neves [UNESP] Beta hemoglobinopathies Beta-thalassemia Sickle cell disease Toxoplasma gondii Toxoplasmosis |
title_short |
Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas |
title_full |
Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas |
title_fullStr |
Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas |
title_full_unstemmed |
Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas |
title_sort |
Anti- Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: The first report in the Americas |
author |
Ferreira, Marina Neves [UNESP] |
author_facet |
Ferreira, Marina Neves [UNESP] Bonini-Domingos, Claudia Regina [UNESP] Fonseca Estevão, Isabeth [UNESP] De Castro Lobo, Clarice Lopes Souza Carrocini, Gisele Cristina [UNESP] Silveira-Carvalho, Aparecida Perpétuo Ricci, Octávio De Mattos, Luiz Carlos Brandão De Mattos, Cinara Cássia |
author_role |
author |
author2 |
Bonini-Domingos, Claudia Regina [UNESP] Fonseca Estevão, Isabeth [UNESP] De Castro Lobo, Clarice Lopes Souza Carrocini, Gisele Cristina [UNESP] Silveira-Carvalho, Aparecida Perpétuo Ricci, Octávio De Mattos, Luiz Carlos Brandão De Mattos, Cinara Cássia |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Faculdade de Medicina de São José Do Rio Preto-FAMERP Faculdade de Medicina de São José Do Rio Preto Instituto de Hematologia Arthur de Siqueira Cavalcanti-HEMORIO Fundação Faculdade Regional de Medicina-FUNFARME Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Ferreira, Marina Neves [UNESP] Bonini-Domingos, Claudia Regina [UNESP] Fonseca Estevão, Isabeth [UNESP] De Castro Lobo, Clarice Lopes Souza Carrocini, Gisele Cristina [UNESP] Silveira-Carvalho, Aparecida Perpétuo Ricci, Octávio De Mattos, Luiz Carlos Brandão De Mattos, Cinara Cássia |
dc.subject.por.fl_str_mv |
Beta hemoglobinopathies Beta-thalassemia Sickle cell disease Toxoplasma gondii Toxoplasmosis |
topic |
Beta hemoglobinopathies Beta-thalassemia Sickle cell disease Toxoplasma gondii Toxoplasmosis |
description |
Background In Brazil, there have been no previous studies of Toxoplasma gondii infection in sickle cell anemia patients and carriers of severe forms of beta-thalassemia. This study evaluated T. gondii infection in patients with beta-hemoglobinopathies. Methods A total of 158 samples, 77 (48.7%) men and 81 (51.3%) women, were evaluated. Three groups were formed: G1 (85 patients with sickle cell disease); G2 (11 patients with homozygous beta-thalassemia; G3 (62 patients with heterozygous beta-thalassemia). ELISA was employed to identify anti-T. gondii IgM and IgG antibodies, and molecular analysis was performed to determine beta-hemoglobin mutations. Fisher's exact test was used to compare frequencies of anti-T. gondii IgM and IgG antibodies in respect to gender and age. Results Anti-T. gondii IgG antibodies were found in 43.5% of individuals in G1, 18.1% in G2 and 50% in G3. All samples from G1 and G2 were seronegative for anti-T. gondii IgM antibodies, but 3.2% from G3 were seropositive. Considering anti-T. gondii IgG antibodies, no statistical significant differences were found between these groups nor in seroprevalence between genders within each group. Despite this, comparisons of the mean ages between G1, G2 and G3 were statistically significant (G2 vs. G1: p value = 0.0001; G3 vs. G1: p-value <0.0001; G3 vs. G2: p-value = 0.0001). Conclusion A comparison by age of patients with sickle cell anemia showed a trend of lower risk of infection among younger individuals. Therefore, this study demonstrates that T. gondii infection occurs in patients with beta-thalassemia and sickle cell anemia in Brazil as seen by the presence of anti-T. gondii IgM and IgG antibodies. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:14:03Z 2018-12-11T17:14:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s13104-017-2535-7 BMC Research Notes, v. 1, n. 1, 2017. 1756-0500 http://hdl.handle.net/11449/175061 10.1186/s13104-017-2535-7 2-s2.0-85027842180 2-s2.0-85027842180.pdf 3279428066176719 0000-0002-4603-9467 |
url |
http://dx.doi.org/10.1186/s13104-017-2535-7 http://hdl.handle.net/11449/175061 |
identifier_str_mv |
BMC Research Notes, v. 1, n. 1, 2017. 1756-0500 10.1186/s13104-017-2535-7 2-s2.0-85027842180 2-s2.0-85027842180.pdf 3279428066176719 0000-0002-4603-9467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Research Notes 0,691 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808128971200528384 |