Identification of hepatic progenitor cells in the canine fetal liver
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2020.09.012 http://hdl.handle.net/11449/209806 |
Resumo: | The liver plays essential roles in human and animal organisms, such as the storage, release, metabolism, and elimination of various endogenous or exogenous substances. Although its vital importance, few treatments are yet available when a hepatic failure occurs, and hence, the use of stem cells has arisen as a possible solution for both human and veterinary medicines. Previous studies have shown the existence of hepatic progenitor cells in human fetuses that were positive for EpCAM and NCAM. There is limited evidence, however, further identification and characterization of these cells in other species. Considering the similarity between dogs and humans regarding physiology, and also the increasing importance of developing new treatments for both veterinary and translational medicine, this study attempted to identify hepatic progenitor cells in canine fetal liver. For that, livers from canine fetuses were collected, cells were isolated by enzymatic digestion and cultured. Cells were characterized regarding morphology and expression of EpCAM, NCAM, Nestin, and Thy-1/CD90 markers. Our results suggest that it is possible to identify hepatic progenitor cells in the canine fetal liver; however, for therapeutic use, further techniques for cellular isolation and culture are necessary to obtain enriched populations of hepatic progenitors from the canine fetal liver. |
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Identification of hepatic progenitor cells in the canine fetal liverLiverEpCAMNCAMDogStem cellsThe liver plays essential roles in human and animal organisms, such as the storage, release, metabolism, and elimination of various endogenous or exogenous substances. Although its vital importance, few treatments are yet available when a hepatic failure occurs, and hence, the use of stem cells has arisen as a possible solution for both human and veterinary medicines. Previous studies have shown the existence of hepatic progenitor cells in human fetuses that were positive for EpCAM and NCAM. There is limited evidence, however, further identification and characterization of these cells in other species. Considering the similarity between dogs and humans regarding physiology, and also the increasing importance of developing new treatments for both veterinary and translational medicine, this study attempted to identify hepatic progenitor cells in canine fetal liver. For that, livers from canine fetuses were collected, cells were isolated by enzymatic digestion and cultured. Cells were characterized regarding morphology and expression of EpCAM, NCAM, Nestin, and Thy-1/CD90 markers. Our results suggest that it is possible to identify hepatic progenitor cells in the canine fetal liver; however, for therapeutic use, further techniques for cellular isolation and culture are necessary to obtain enriched populations of hepatic progenitors from the canine fetal liver.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)State Univ Sao Paulo, Fac Agr & Anim Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, BrazilUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP, BrazilState Univ Sao Paulo, Fac Agr & Anim Sci, Dept Vet Clin & Surg, Jaboticabal, SP, BrazilUniv Sao Paulo, Fac Vet Med & Anim Sci, Postgrad Program Anat Domest & Wild Anim, Sao Paulo, SP, BrazilState Univ Sao Paulo, Fac Agr & Anim Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, BrazilState Univ Sao Paulo, Fac Agr & Anim Sci, Dept Vet Clin & Surg, Jaboticabal, SP, BrazilFAPESP: 2017/21266-0Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Tavares, M. R. [UNESP]Castro, R. V. G. de [UNESP]Pieri, N. C. G.Cruz, N. R. N. [UNESP]Martins, D. S.Ambrosio, C. E.Garcia, J. M. [UNESP]Camplesi, A. C. [UNESP]Bressan, F. F.Toniollo, G. H. [UNESP]2021-06-25T12:29:53Z2021-06-25T12:29:53Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article239-245http://dx.doi.org/10.1016/j.rvsc.2020.09.012Research In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 133, p. 239-245, 2020.0034-5288http://hdl.handle.net/11449/20980610.1016/j.rvsc.2020.09.012WOS:000600568400006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch In Veterinary Scienceinfo:eu-repo/semantics/openAccess2021-10-23T19:50:02Zoai:repositorio.unesp.br:11449/209806Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:50:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of hepatic progenitor cells in the canine fetal liver |
title |
Identification of hepatic progenitor cells in the canine fetal liver |
spellingShingle |
Identification of hepatic progenitor cells in the canine fetal liver Tavares, M. R. [UNESP] Liver EpCAM NCAM Dog Stem cells |
title_short |
Identification of hepatic progenitor cells in the canine fetal liver |
title_full |
Identification of hepatic progenitor cells in the canine fetal liver |
title_fullStr |
Identification of hepatic progenitor cells in the canine fetal liver |
title_full_unstemmed |
Identification of hepatic progenitor cells in the canine fetal liver |
title_sort |
Identification of hepatic progenitor cells in the canine fetal liver |
author |
Tavares, M. R. [UNESP] |
author_facet |
Tavares, M. R. [UNESP] Castro, R. V. G. de [UNESP] Pieri, N. C. G. Cruz, N. R. N. [UNESP] Martins, D. S. Ambrosio, C. E. Garcia, J. M. [UNESP] Camplesi, A. C. [UNESP] Bressan, F. F. Toniollo, G. H. [UNESP] |
author_role |
author |
author2 |
Castro, R. V. G. de [UNESP] Pieri, N. C. G. Cruz, N. R. N. [UNESP] Martins, D. S. Ambrosio, C. E. Garcia, J. M. [UNESP] Camplesi, A. C. [UNESP] Bressan, F. F. Toniollo, G. H. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Tavares, M. R. [UNESP] Castro, R. V. G. de [UNESP] Pieri, N. C. G. Cruz, N. R. N. [UNESP] Martins, D. S. Ambrosio, C. E. Garcia, J. M. [UNESP] Camplesi, A. C. [UNESP] Bressan, F. F. Toniollo, G. H. [UNESP] |
dc.subject.por.fl_str_mv |
Liver EpCAM NCAM Dog Stem cells |
topic |
Liver EpCAM NCAM Dog Stem cells |
description |
The liver plays essential roles in human and animal organisms, such as the storage, release, metabolism, and elimination of various endogenous or exogenous substances. Although its vital importance, few treatments are yet available when a hepatic failure occurs, and hence, the use of stem cells has arisen as a possible solution for both human and veterinary medicines. Previous studies have shown the existence of hepatic progenitor cells in human fetuses that were positive for EpCAM and NCAM. There is limited evidence, however, further identification and characterization of these cells in other species. Considering the similarity between dogs and humans regarding physiology, and also the increasing importance of developing new treatments for both veterinary and translational medicine, this study attempted to identify hepatic progenitor cells in canine fetal liver. For that, livers from canine fetuses were collected, cells were isolated by enzymatic digestion and cultured. Cells were characterized regarding morphology and expression of EpCAM, NCAM, Nestin, and Thy-1/CD90 markers. Our results suggest that it is possible to identify hepatic progenitor cells in the canine fetal liver; however, for therapeutic use, further techniques for cellular isolation and culture are necessary to obtain enriched populations of hepatic progenitors from the canine fetal liver. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2021-06-25T12:29:53Z 2021-06-25T12:29:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2020.09.012 Research In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 133, p. 239-245, 2020. 0034-5288 http://hdl.handle.net/11449/209806 10.1016/j.rvsc.2020.09.012 WOS:000600568400006 |
url |
http://dx.doi.org/10.1016/j.rvsc.2020.09.012 http://hdl.handle.net/11449/209806 |
identifier_str_mv |
Research In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 133, p. 239-245, 2020. 0034-5288 10.1016/j.rvsc.2020.09.012 WOS:000600568400006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research In Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
239-245 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965128426258432 |