Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3109/13880209.2015.1120321 http://hdl.handle.net/11449/172529 |
Resumo: | Context Ethnopharmacological studies have demonstrated that plants of the Combretum genus presented antidiabetic activity, including Combretum lanceolatum Pohl ex Eichler (Combretaceae). Objective This study investigated the hepatic mechanisms of action of C. lanceolatum flowers ethanol extract (ClEtOH) related to its antihyperglycaemic effect in streptozotocin-diabetic rats. Materials and methods Male Wistar rats were divided into normal (N) and diabetic control (DC) rats treated with vehicle (water); diabetic rats treated with 500 mg/kg metformin (DMet) or 500 mg/kg ClEtOH (DT500). After 21 d of treatment, hepatic glucose and urea production were investigated through in situ perfused liver with l-glutamine. Changes in the phosphoenolpyruvate carboxykinase (PEPCK) levels and in the activation of adenosine monophosphate-activated protein kinase (AMPK) and insulin-signalling intermediates were also investigated. Results Similar to DMet, DT500 rats showed a reduction in the rates of hepatic production of glucose (46%) and urea (22%) in comparison with DC. This reduction was accompanied by a reduction in the PEPCK levels in liver of DT500 (28%) and DMet (43%) when compared with DC. AMPK phosphorylation levels were higher in the liver of DT500 (17%) and DMet (16%) rats. The basal AKT phosphorylation levels were increased in liver of DT500 rats, without differences in the insulin-stimulated AKT phosphorylation and in the insulin receptor levels between DC and DT500 rats. Discussion and conclusion The antidiabetic activity of ClEtOH can be attributed, at least in part, to inhibition of hepatic gluconeogenesis, probably due to the activation of both AMPK and AKT effectors and reduction in the PEPCK levels. |
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Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism studyAntidiabetic activityglucose hepatic productionphosphoenolpyruvate carboxykinaseContext Ethnopharmacological studies have demonstrated that plants of the Combretum genus presented antidiabetic activity, including Combretum lanceolatum Pohl ex Eichler (Combretaceae). Objective This study investigated the hepatic mechanisms of action of C. lanceolatum flowers ethanol extract (ClEtOH) related to its antihyperglycaemic effect in streptozotocin-diabetic rats. Materials and methods Male Wistar rats were divided into normal (N) and diabetic control (DC) rats treated with vehicle (water); diabetic rats treated with 500 mg/kg metformin (DMet) or 500 mg/kg ClEtOH (DT500). After 21 d of treatment, hepatic glucose and urea production were investigated through in situ perfused liver with l-glutamine. Changes in the phosphoenolpyruvate carboxykinase (PEPCK) levels and in the activation of adenosine monophosphate-activated protein kinase (AMPK) and insulin-signalling intermediates were also investigated. Results Similar to DMet, DT500 rats showed a reduction in the rates of hepatic production of glucose (46%) and urea (22%) in comparison with DC. This reduction was accompanied by a reduction in the PEPCK levels in liver of DT500 (28%) and DMet (43%) when compared with DC. AMPK phosphorylation levels were higher in the liver of DT500 (17%) and DMet (16%) rats. The basal AKT phosphorylation levels were increased in liver of DT500 rats, without differences in the insulin-stimulated AKT phosphorylation and in the insulin receptor levels between DC and DT500 rats. Discussion and conclusion The antidiabetic activity of ClEtOH can be attributed, at least in part, to inhibition of hepatic gluconeogenesis, probably due to the activation of both AMPK and AKT effectors and reduction in the PEPCK levels.Department of Chemistry Federal University of Mato GrossoDepartment of Physiological Sciences State University of LondrinaDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University UNESPDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University UNESPFederal University of Mato GrossoUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Siqueira, Juliany TorresBatistela, EmanuelePereira, Mayara Peronda Silva, Virginia Claudiade Sousa Junior, Paulo TeixeiraAndrade, Claudia Marlise BalbinottiKawashita, Nair HondaBertolini, Gisele LopesBaviera, Amanda Martins [UNESP]2018-12-11T17:00:49Z2018-12-11T17:00:49Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1671-1679application/pdfhttp://dx.doi.org/10.3109/13880209.2015.1120321Pharmaceutical Biology, v. 54, n. 9, p. 1671-1679, 2016.1744-51161388-0209http://hdl.handle.net/11449/17252910.3109/13880209.2015.11203212-s2.0-849580612912-s2.0-84958061291.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmaceutical Biology0,5630,563info:eu-repo/semantics/openAccess2024-06-21T15:18:56Zoai:repositorio.unesp.br:11449/172529Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:15:41.563011Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study |
title |
Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study |
spellingShingle |
Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study Siqueira, Juliany Torres Antidiabetic activity glucose hepatic production phosphoenolpyruvate carboxykinase |
title_short |
Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study |
title_full |
Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study |
title_fullStr |
Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study |
title_full_unstemmed |
Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study |
title_sort |
Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study |
author |
Siqueira, Juliany Torres |
author_facet |
Siqueira, Juliany Torres Batistela, Emanuele Pereira, Mayara Peron da Silva, Virginia Claudia de Sousa Junior, Paulo Teixeira Andrade, Claudia Marlise Balbinotti Kawashita, Nair Honda Bertolini, Gisele Lopes Baviera, Amanda Martins [UNESP] |
author_role |
author |
author2 |
Batistela, Emanuele Pereira, Mayara Peron da Silva, Virginia Claudia de Sousa Junior, Paulo Teixeira Andrade, Claudia Marlise Balbinotti Kawashita, Nair Honda Bertolini, Gisele Lopes Baviera, Amanda Martins [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Mato Grosso Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Siqueira, Juliany Torres Batistela, Emanuele Pereira, Mayara Peron da Silva, Virginia Claudia de Sousa Junior, Paulo Teixeira Andrade, Claudia Marlise Balbinotti Kawashita, Nair Honda Bertolini, Gisele Lopes Baviera, Amanda Martins [UNESP] |
dc.subject.por.fl_str_mv |
Antidiabetic activity glucose hepatic production phosphoenolpyruvate carboxykinase |
topic |
Antidiabetic activity glucose hepatic production phosphoenolpyruvate carboxykinase |
description |
Context Ethnopharmacological studies have demonstrated that plants of the Combretum genus presented antidiabetic activity, including Combretum lanceolatum Pohl ex Eichler (Combretaceae). Objective This study investigated the hepatic mechanisms of action of C. lanceolatum flowers ethanol extract (ClEtOH) related to its antihyperglycaemic effect in streptozotocin-diabetic rats. Materials and methods Male Wistar rats were divided into normal (N) and diabetic control (DC) rats treated with vehicle (water); diabetic rats treated with 500 mg/kg metformin (DMet) or 500 mg/kg ClEtOH (DT500). After 21 d of treatment, hepatic glucose and urea production were investigated through in situ perfused liver with l-glutamine. Changes in the phosphoenolpyruvate carboxykinase (PEPCK) levels and in the activation of adenosine monophosphate-activated protein kinase (AMPK) and insulin-signalling intermediates were also investigated. Results Similar to DMet, DT500 rats showed a reduction in the rates of hepatic production of glucose (46%) and urea (22%) in comparison with DC. This reduction was accompanied by a reduction in the PEPCK levels in liver of DT500 (28%) and DMet (43%) when compared with DC. AMPK phosphorylation levels were higher in the liver of DT500 (17%) and DMet (16%) rats. The basal AKT phosphorylation levels were increased in liver of DT500 rats, without differences in the insulin-stimulated AKT phosphorylation and in the insulin receptor levels between DC and DT500 rats. Discussion and conclusion The antidiabetic activity of ClEtOH can be attributed, at least in part, to inhibition of hepatic gluconeogenesis, probably due to the activation of both AMPK and AKT effectors and reduction in the PEPCK levels. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-12-11T17:00:49Z 2018-12-11T17:00:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3109/13880209.2015.1120321 Pharmaceutical Biology, v. 54, n. 9, p. 1671-1679, 2016. 1744-5116 1388-0209 http://hdl.handle.net/11449/172529 10.3109/13880209.2015.1120321 2-s2.0-84958061291 2-s2.0-84958061291.pdf |
url |
http://dx.doi.org/10.3109/13880209.2015.1120321 http://hdl.handle.net/11449/172529 |
identifier_str_mv |
Pharmaceutical Biology, v. 54, n. 9, p. 1671-1679, 2016. 1744-5116 1388-0209 10.3109/13880209.2015.1120321 2-s2.0-84958061291 2-s2.0-84958061291.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmaceutical Biology 0,563 0,563 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1671-1679 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128911894118400 |