Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria

Detalhes bibliográficos
Autor(a) principal: Pacheco, Rodrigo D. L. [UNESP]
Data de Publicação: 2023
Outros Autores: Souza, Johnny M. [UNESP], Marino, Carolina T., Bastos, João Paulo S. T. [UNESP], Martins, Cyntia L. [UNESP], Rodrigues, Paulo H. M., Arrigoni, Mario D. B. [UNESP], Millen, Danilo D. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fvets.2023.1090107
http://hdl.handle.net/11449/248693
Resumo: This study was designed to evaluate a spray-dried multivalent polyclonal antibody preparation (PAP) against lactate-producing bacteria as an alternative to monensin (MON) to control ruminal acidification. Holstein cows (677 ± 98 kg) fitted with ruminal cannulas were allocated in an incomplete Latin square design with two 20 days period. Cows were randomly assigned to control (CTL), PAP, or MON treatments. For each period, cows were fed a forage diet in the first 5 days (d−5 to d−1), composed of sugarcane, urea and a mineral supplement, followed by a 74% concentrate diet for 15 days (d 0 to d 14). There were no treatment main effects (P > 0.05) on dry matter intake (DMI) and microbial protein synthesis. However, there was a large peak (P < 0.01) of intake on d 0 (18.29 kg), followed by a large decline on d 1 (3.67 kg). From d2, DMI showed an increasing pattern (8.34 kg) and stabilized around d 8 (12.96 kg). Higher mean pH was measured (P < 0.01) in cattle-fed MON (6.06 vs. PAP = 5.89 and CTL = 5.91). The ruminal NH3-N concentration of CTL-fed cows was lower (P < 0.01) compared to those fed MON or PAP. The molar concentration of acetate and lactate was not affected (P > 0.23) by treatments, but feeding MON increased (P = 0.01) propionate during the first 4 days after the challenge. Feeding MON and PAP reduced (P = 0.01) the molar proportion of butyrate. MON was effective in controlling pH and improved ruminal fermentation of acidosis-induced cows. However, PAP was not effective in controlling acidosis. The acidosis induced by the challenge was caused by the accumulation of SCFAs. Therefore, the real conditions for evaluation of this feed additive were not reached in this experiment, since this PAP was proposed to work against lactate-producing bacteria.
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spelling Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteriaadditivesFusobacterium necrophorumLactobacillus ssprumenStreptococcus bovisThis study was designed to evaluate a spray-dried multivalent polyclonal antibody preparation (PAP) against lactate-producing bacteria as an alternative to monensin (MON) to control ruminal acidification. Holstein cows (677 ± 98 kg) fitted with ruminal cannulas were allocated in an incomplete Latin square design with two 20 days period. Cows were randomly assigned to control (CTL), PAP, or MON treatments. For each period, cows were fed a forage diet in the first 5 days (d−5 to d−1), composed of sugarcane, urea and a mineral supplement, followed by a 74% concentrate diet for 15 days (d 0 to d 14). There were no treatment main effects (P > 0.05) on dry matter intake (DMI) and microbial protein synthesis. However, there was a large peak (P < 0.01) of intake on d 0 (18.29 kg), followed by a large decline on d 1 (3.67 kg). From d2, DMI showed an increasing pattern (8.34 kg) and stabilized around d 8 (12.96 kg). Higher mean pH was measured (P < 0.01) in cattle-fed MON (6.06 vs. PAP = 5.89 and CTL = 5.91). The ruminal NH3-N concentration of CTL-fed cows was lower (P < 0.01) compared to those fed MON or PAP. The molar concentration of acetate and lactate was not affected (P > 0.23) by treatments, but feeding MON increased (P = 0.01) propionate during the first 4 days after the challenge. Feeding MON and PAP reduced (P = 0.01) the molar proportion of butyrate. MON was effective in controlling pH and improved ruminal fermentation of acidosis-induced cows. However, PAP was not effective in controlling acidosis. The acidosis induced by the challenge was caused by the accumulation of SCFAs. Therefore, the real conditions for evaluation of this feed additive were not reached in this experiment, since this PAP was proposed to work against lactate-producing bacteria.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Breeding and Animal Nutrition School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Department of Animal Production College of Agricultural and Technological Sciences São Paulo State University (UNESP)Embrapa Beef CattleSchool of Veterinary Medicine and Animal Science University of São PauloDepartment of Breeding and Animal Nutrition School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Department of Animal Production College of Agricultural and Technological Sciences São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade de São Paulo (USP)Pacheco, Rodrigo D. L. [UNESP]Souza, Johnny M. [UNESP]Marino, Carolina T.Bastos, João Paulo S. T. [UNESP]Martins, Cyntia L. [UNESP]Rodrigues, Paulo H. M.Arrigoni, Mario D. B. [UNESP]Millen, Danilo D. [UNESP]2023-07-29T13:50:57Z2023-07-29T13:50:57Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fvets.2023.1090107Frontiers in Veterinary Science, v. 10.2297-1769http://hdl.handle.net/11449/24869310.3389/fvets.2023.10901072-s2.0-85152583836Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Veterinary Scienceinfo:eu-repo/semantics/openAccess2024-09-06T18:55:50Zoai:repositorio.unesp.br:11449/248693Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-06T18:55:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria
title Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria
spellingShingle Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria
Pacheco, Rodrigo D. L. [UNESP]
additives
Fusobacterium necrophorum
Lactobacillus ssp
rumen
Streptococcus bovis
title_short Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria
title_full Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria
title_fullStr Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria
title_full_unstemmed Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria
title_sort Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria
author Pacheco, Rodrigo D. L. [UNESP]
author_facet Pacheco, Rodrigo D. L. [UNESP]
Souza, Johnny M. [UNESP]
Marino, Carolina T.
Bastos, João Paulo S. T. [UNESP]
Martins, Cyntia L. [UNESP]
Rodrigues, Paulo H. M.
Arrigoni, Mario D. B. [UNESP]
Millen, Danilo D. [UNESP]
author_role author
author2 Souza, Johnny M. [UNESP]
Marino, Carolina T.
Bastos, João Paulo S. T. [UNESP]
Martins, Cyntia L. [UNESP]
Rodrigues, Paulo H. M.
Arrigoni, Mario D. B. [UNESP]
Millen, Danilo D. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Pacheco, Rodrigo D. L. [UNESP]
Souza, Johnny M. [UNESP]
Marino, Carolina T.
Bastos, João Paulo S. T. [UNESP]
Martins, Cyntia L. [UNESP]
Rodrigues, Paulo H. M.
Arrigoni, Mario D. B. [UNESP]
Millen, Danilo D. [UNESP]
dc.subject.por.fl_str_mv additives
Fusobacterium necrophorum
Lactobacillus ssp
rumen
Streptococcus bovis
topic additives
Fusobacterium necrophorum
Lactobacillus ssp
rumen
Streptococcus bovis
description This study was designed to evaluate a spray-dried multivalent polyclonal antibody preparation (PAP) against lactate-producing bacteria as an alternative to monensin (MON) to control ruminal acidification. Holstein cows (677 ± 98 kg) fitted with ruminal cannulas were allocated in an incomplete Latin square design with two 20 days period. Cows were randomly assigned to control (CTL), PAP, or MON treatments. For each period, cows were fed a forage diet in the first 5 days (d−5 to d−1), composed of sugarcane, urea and a mineral supplement, followed by a 74% concentrate diet for 15 days (d 0 to d 14). There were no treatment main effects (P > 0.05) on dry matter intake (DMI) and microbial protein synthesis. However, there was a large peak (P < 0.01) of intake on d 0 (18.29 kg), followed by a large decline on d 1 (3.67 kg). From d2, DMI showed an increasing pattern (8.34 kg) and stabilized around d 8 (12.96 kg). Higher mean pH was measured (P < 0.01) in cattle-fed MON (6.06 vs. PAP = 5.89 and CTL = 5.91). The ruminal NH3-N concentration of CTL-fed cows was lower (P < 0.01) compared to those fed MON or PAP. The molar concentration of acetate and lactate was not affected (P > 0.23) by treatments, but feeding MON increased (P = 0.01) propionate during the first 4 days after the challenge. Feeding MON and PAP reduced (P = 0.01) the molar proportion of butyrate. MON was effective in controlling pH and improved ruminal fermentation of acidosis-induced cows. However, PAP was not effective in controlling acidosis. The acidosis induced by the challenge was caused by the accumulation of SCFAs. Therefore, the real conditions for evaluation of this feed additive were not reached in this experiment, since this PAP was proposed to work against lactate-producing bacteria.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:50:57Z
2023-07-29T13:50:57Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fvets.2023.1090107
Frontiers in Veterinary Science, v. 10.
2297-1769
http://hdl.handle.net/11449/248693
10.3389/fvets.2023.1090107
2-s2.0-85152583836
url http://dx.doi.org/10.3389/fvets.2023.1090107
http://hdl.handle.net/11449/248693
identifier_str_mv Frontiers in Veterinary Science, v. 10.
2297-1769
10.3389/fvets.2023.1090107
2-s2.0-85152583836
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Veterinary Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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