Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fvets.2023.1090107 http://hdl.handle.net/11449/248693 |
Resumo: | This study was designed to evaluate a spray-dried multivalent polyclonal antibody preparation (PAP) against lactate-producing bacteria as an alternative to monensin (MON) to control ruminal acidification. Holstein cows (677 ± 98 kg) fitted with ruminal cannulas were allocated in an incomplete Latin square design with two 20 days period. Cows were randomly assigned to control (CTL), PAP, or MON treatments. For each period, cows were fed a forage diet in the first 5 days (d−5 to d−1), composed of sugarcane, urea and a mineral supplement, followed by a 74% concentrate diet for 15 days (d 0 to d 14). There were no treatment main effects (P > 0.05) on dry matter intake (DMI) and microbial protein synthesis. However, there was a large peak (P < 0.01) of intake on d 0 (18.29 kg), followed by a large decline on d 1 (3.67 kg). From d2, DMI showed an increasing pattern (8.34 kg) and stabilized around d 8 (12.96 kg). Higher mean pH was measured (P < 0.01) in cattle-fed MON (6.06 vs. PAP = 5.89 and CTL = 5.91). The ruminal NH3-N concentration of CTL-fed cows was lower (P < 0.01) compared to those fed MON or PAP. The molar concentration of acetate and lactate was not affected (P > 0.23) by treatments, but feeding MON increased (P = 0.01) propionate during the first 4 days after the challenge. Feeding MON and PAP reduced (P = 0.01) the molar proportion of butyrate. MON was effective in controlling pH and improved ruminal fermentation of acidosis-induced cows. However, PAP was not effective in controlling acidosis. The acidosis induced by the challenge was caused by the accumulation of SCFAs. Therefore, the real conditions for evaluation of this feed additive were not reached in this experiment, since this PAP was proposed to work against lactate-producing bacteria. |
id |
UNSP_75f7edcdefecfacf044a7f04a4952b5e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/248693 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteriaadditivesFusobacterium necrophorumLactobacillus ssprumenStreptococcus bovisThis study was designed to evaluate a spray-dried multivalent polyclonal antibody preparation (PAP) against lactate-producing bacteria as an alternative to monensin (MON) to control ruminal acidification. Holstein cows (677 ± 98 kg) fitted with ruminal cannulas were allocated in an incomplete Latin square design with two 20 days period. Cows were randomly assigned to control (CTL), PAP, or MON treatments. For each period, cows were fed a forage diet in the first 5 days (d−5 to d−1), composed of sugarcane, urea and a mineral supplement, followed by a 74% concentrate diet for 15 days (d 0 to d 14). There were no treatment main effects (P > 0.05) on dry matter intake (DMI) and microbial protein synthesis. However, there was a large peak (P < 0.01) of intake on d 0 (18.29 kg), followed by a large decline on d 1 (3.67 kg). From d2, DMI showed an increasing pattern (8.34 kg) and stabilized around d 8 (12.96 kg). Higher mean pH was measured (P < 0.01) in cattle-fed MON (6.06 vs. PAP = 5.89 and CTL = 5.91). The ruminal NH3-N concentration of CTL-fed cows was lower (P < 0.01) compared to those fed MON or PAP. The molar concentration of acetate and lactate was not affected (P > 0.23) by treatments, but feeding MON increased (P = 0.01) propionate during the first 4 days after the challenge. Feeding MON and PAP reduced (P = 0.01) the molar proportion of butyrate. MON was effective in controlling pH and improved ruminal fermentation of acidosis-induced cows. However, PAP was not effective in controlling acidosis. The acidosis induced by the challenge was caused by the accumulation of SCFAs. Therefore, the real conditions for evaluation of this feed additive were not reached in this experiment, since this PAP was proposed to work against lactate-producing bacteria.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Breeding and Animal Nutrition School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Department of Animal Production College of Agricultural and Technological Sciences São Paulo State University (UNESP)Embrapa Beef CattleSchool of Veterinary Medicine and Animal Science University of São PauloDepartment of Breeding and Animal Nutrition School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Department of Animal Production College of Agricultural and Technological Sciences São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade de São Paulo (USP)Pacheco, Rodrigo D. L. [UNESP]Souza, Johnny M. [UNESP]Marino, Carolina T.Bastos, João Paulo S. T. [UNESP]Martins, Cyntia L. [UNESP]Rodrigues, Paulo H. M.Arrigoni, Mario D. B. [UNESP]Millen, Danilo D. [UNESP]2023-07-29T13:50:57Z2023-07-29T13:50:57Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fvets.2023.1090107Frontiers in Veterinary Science, v. 10.2297-1769http://hdl.handle.net/11449/24869310.3389/fvets.2023.10901072-s2.0-85152583836Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Veterinary Scienceinfo:eu-repo/semantics/openAccess2024-09-06T18:55:50Zoai:repositorio.unesp.br:11449/248693Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-06T18:55:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria |
title |
Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria |
spellingShingle |
Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria Pacheco, Rodrigo D. L. [UNESP] additives Fusobacterium necrophorum Lactobacillus ssp rumen Streptococcus bovis |
title_short |
Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria |
title_full |
Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria |
title_fullStr |
Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria |
title_full_unstemmed |
Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria |
title_sort |
Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria |
author |
Pacheco, Rodrigo D. L. [UNESP] |
author_facet |
Pacheco, Rodrigo D. L. [UNESP] Souza, Johnny M. [UNESP] Marino, Carolina T. Bastos, João Paulo S. T. [UNESP] Martins, Cyntia L. [UNESP] Rodrigues, Paulo H. M. Arrigoni, Mario D. B. [UNESP] Millen, Danilo D. [UNESP] |
author_role |
author |
author2 |
Souza, Johnny M. [UNESP] Marino, Carolina T. Bastos, João Paulo S. T. [UNESP] Martins, Cyntia L. [UNESP] Rodrigues, Paulo H. M. Arrigoni, Mario D. B. [UNESP] Millen, Danilo D. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Pacheco, Rodrigo D. L. [UNESP] Souza, Johnny M. [UNESP] Marino, Carolina T. Bastos, João Paulo S. T. [UNESP] Martins, Cyntia L. [UNESP] Rodrigues, Paulo H. M. Arrigoni, Mario D. B. [UNESP] Millen, Danilo D. [UNESP] |
dc.subject.por.fl_str_mv |
additives Fusobacterium necrophorum Lactobacillus ssp rumen Streptococcus bovis |
topic |
additives Fusobacterium necrophorum Lactobacillus ssp rumen Streptococcus bovis |
description |
This study was designed to evaluate a spray-dried multivalent polyclonal antibody preparation (PAP) against lactate-producing bacteria as an alternative to monensin (MON) to control ruminal acidification. Holstein cows (677 ± 98 kg) fitted with ruminal cannulas were allocated in an incomplete Latin square design with two 20 days period. Cows were randomly assigned to control (CTL), PAP, or MON treatments. For each period, cows were fed a forage diet in the first 5 days (d−5 to d−1), composed of sugarcane, urea and a mineral supplement, followed by a 74% concentrate diet for 15 days (d 0 to d 14). There were no treatment main effects (P > 0.05) on dry matter intake (DMI) and microbial protein synthesis. However, there was a large peak (P < 0.01) of intake on d 0 (18.29 kg), followed by a large decline on d 1 (3.67 kg). From d2, DMI showed an increasing pattern (8.34 kg) and stabilized around d 8 (12.96 kg). Higher mean pH was measured (P < 0.01) in cattle-fed MON (6.06 vs. PAP = 5.89 and CTL = 5.91). The ruminal NH3-N concentration of CTL-fed cows was lower (P < 0.01) compared to those fed MON or PAP. The molar concentration of acetate and lactate was not affected (P > 0.23) by treatments, but feeding MON increased (P = 0.01) propionate during the first 4 days after the challenge. Feeding MON and PAP reduced (P = 0.01) the molar proportion of butyrate. MON was effective in controlling pH and improved ruminal fermentation of acidosis-induced cows. However, PAP was not effective in controlling acidosis. The acidosis induced by the challenge was caused by the accumulation of SCFAs. Therefore, the real conditions for evaluation of this feed additive were not reached in this experiment, since this PAP was proposed to work against lactate-producing bacteria. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:50:57Z 2023-07-29T13:50:57Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fvets.2023.1090107 Frontiers in Veterinary Science, v. 10. 2297-1769 http://hdl.handle.net/11449/248693 10.3389/fvets.2023.1090107 2-s2.0-85152583836 |
url |
http://dx.doi.org/10.3389/fvets.2023.1090107 http://hdl.handle.net/11449/248693 |
identifier_str_mv |
Frontiers in Veterinary Science, v. 10. 2297-1769 10.3389/fvets.2023.1090107 2-s2.0-85152583836 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546615044046848 |