Effect of sodium bicarbonate on Candida albicans adherence to thermally activated acrylic resin

• Autor(a) principal: de Sousa, Fernando Augusto Cervantes Garcia [UNESP]
• Data de Publicação: 2009
• Outros Autores: Paradella, Thaís Cachuté [UNESP], Koga-Ito, Cristiane Yumi [UNESP], Jorge, Antonio Olavo Cardoso [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S1806-83242009000400006; http://hdl.handle.net/11449/71182
• topic: Acrylic resins; Candida albicans; Cell adhesion; Sodium bicarbonate; acrylic acid resin; bicarbonate; disinfectant agent; toothpaste; bacterial count; chemistry; denture; drug effect; heat; isolation and purification; materials testing; microbiology; nonparametric test; stomatitis; surface property; time; Acrylic Resins; Colony Count, Microbial; Dental Disinfectants; Denture Cleansers; Denture, Complete; Hot Temperature; Materials Testing; Sodium Bicarbonate; Statistics, Nonparametric; Stomatitis, Denture; Surface Properties; Time Factors

The purpose of this study was to evaluate the effect of 5% sodium bicarbonate on the adherence of Candida albicans to thermally activated acrylic resin. Fifty 4 mm specimens of acrylic resin were obtained using a metallic matrix. The specimens received chemical polishing, were sterilized and then immersed in Sabouraud broth, inoculated with Candida albicans standardized suspension. After 24 hours of incubation at 37°C, the specimens were divided into four groups according to the substance used for disinfection (5% sodium bicarbonate, 0.12% digluconate chlorhexidine, vinegar and Corega Tabs). A control group was included, in which distilled water was used. The adhered microorganisms were dispersed, diluted and plated onto culture media to determine the number of colony-forming units (cfu/mL). The results were analyzed through the Mann-Whitney statistical test at the 5% level of significance. Only 0.12% digluconate chlorhexidine and 5% sodium bicarbonate presented a statistically significant difference (p = 0.0010 and p = 0.0156, respectively) compared to the control group, decreasing the number of cfu/mL. However, when the different disinfecting solutions were compared with each other, only 0.12% digluconate chlorhexidine presented a statistically significant difference in the reduction of cfu/mL. It was concluded that although 0.12% digluconate chlorhexidine was more effective in the reduction of Candida albicans adherence values to thermally activated acrylic resin, 5% sodium bicarbonate also proved to be a viable alternative.