Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1051/analusis:1998122 http://hdl.handle.net/11449/31290 |
Resumo: | Enzyme-Linked Immunosorbent Assay (ELISA) has been evaluated by analyzing rich-humic water samples from tropical rivers. The samples were spiked with atrazine at ppb level Different pHs (4 to 9) and humic concentrations (2.5 to 40 mg L-1) were investigated. The assay performance showed a strong dependence on the pH values and amount of humic matter at low atrazine concentration. From all the conditions studied the low pH (pH 4) and high humic substances concentrations (40 mg L-1) showed the greatest influence. The IC50 value to control sample (no humic) diminished from 0.28 nmol L-1 to 0.64 nmol L-1 to humic acid solution. This effect is specially noted for the humic acid fractions, since fulvic acid fractions showed no significant change on the immunoassay results. Additionally, it has been demonstrated that at basic pH the matrix effect produced by the natural Brazilian water sample containing humic substances even at 40 mg L-1 disappears. Therefore, the ELISA method used to determine atrazine, can be employed to determine this pesticide in water samples containing humic substances without prior preparation. |
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Repositório Institucional da UNESP |
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Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assayatrazineaquatic humic substancesimmunoassaysEnzyme-Linked Immunosorbent Assay (ELISA) has been evaluated by analyzing rich-humic water samples from tropical rivers. The samples were spiked with atrazine at ppb level Different pHs (4 to 9) and humic concentrations (2.5 to 40 mg L-1) were investigated. The assay performance showed a strong dependence on the pH values and amount of humic matter at low atrazine concentration. From all the conditions studied the low pH (pH 4) and high humic substances concentrations (40 mg L-1) showed the greatest influence. The IC50 value to control sample (no humic) diminished from 0.28 nmol L-1 to 0.64 nmol L-1 to humic acid solution. This effect is specially noted for the humic acid fractions, since fulvic acid fractions showed no significant change on the immunoassay results. Additionally, it has been demonstrated that at basic pH the matrix effect produced by the natural Brazilian water sample containing humic substances even at 40 mg L-1 disappears. Therefore, the ELISA method used to determine atrazine, can be employed to determine this pesticide in water samples containing humic substances without prior preparation.CSIC, Cid, Dept Environm Chem, ES-08034 Barcelona, SpainCSIC, Cid, Dept Organ & Biol Chem, ES-08034 Barcelona, SpainUNESP, Dept Analyt Chem, BR-14800900 Araraquara, BrazilUNESP, Dept Analyt Chem, BR-14800900 Araraquara, BrazilE D P SciencesCSICUniversidade Estadual Paulista (Unesp)Toscano, IGascon, J.Marco, M. P.Rocha, Julio Cesar [UNESP]Barcelo, D.2014-05-20T15:19:54Z2014-05-20T15:19:54Z1998-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article130-134application/pdfhttp://dx.doi.org/10.1051/analusis:1998122Analusis. Les Ulis Cedexa: E D P Sciences, v. 26, n. 3, p. 130-134, 1998.0365-4877http://hdl.handle.net/11449/3129010.1051/analusis:1998122WOS:000073612000015WOS000073612000015.pdf4148837403618207Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnalusisinfo:eu-repo/semantics/openAccess2023-10-27T06:10:39Zoai:repositorio.unesp.br:11449/31290Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-27T06:10:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay |
title |
Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay |
spellingShingle |
Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay Toscano, I atrazine aquatic humic substances immunoassays |
title_short |
Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay |
title_full |
Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay |
title_fullStr |
Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay |
title_full_unstemmed |
Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay |
title_sort |
Atrazine interaction with tropical humic substances by Enzyme Linked Immunosorbent Assay |
author |
Toscano, I |
author_facet |
Toscano, I Gascon, J. Marco, M. P. Rocha, Julio Cesar [UNESP] Barcelo, D. |
author_role |
author |
author2 |
Gascon, J. Marco, M. P. Rocha, Julio Cesar [UNESP] Barcelo, D. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
CSIC Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Toscano, I Gascon, J. Marco, M. P. Rocha, Julio Cesar [UNESP] Barcelo, D. |
dc.subject.por.fl_str_mv |
atrazine aquatic humic substances immunoassays |
topic |
atrazine aquatic humic substances immunoassays |
description |
Enzyme-Linked Immunosorbent Assay (ELISA) has been evaluated by analyzing rich-humic water samples from tropical rivers. The samples were spiked with atrazine at ppb level Different pHs (4 to 9) and humic concentrations (2.5 to 40 mg L-1) were investigated. The assay performance showed a strong dependence on the pH values and amount of humic matter at low atrazine concentration. From all the conditions studied the low pH (pH 4) and high humic substances concentrations (40 mg L-1) showed the greatest influence. The IC50 value to control sample (no humic) diminished from 0.28 nmol L-1 to 0.64 nmol L-1 to humic acid solution. This effect is specially noted for the humic acid fractions, since fulvic acid fractions showed no significant change on the immunoassay results. Additionally, it has been demonstrated that at basic pH the matrix effect produced by the natural Brazilian water sample containing humic substances even at 40 mg L-1 disappears. Therefore, the ELISA method used to determine atrazine, can be employed to determine this pesticide in water samples containing humic substances without prior preparation. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-04-01 2014-05-20T15:19:54Z 2014-05-20T15:19:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1051/analusis:1998122 Analusis. Les Ulis Cedexa: E D P Sciences, v. 26, n. 3, p. 130-134, 1998. 0365-4877 http://hdl.handle.net/11449/31290 10.1051/analusis:1998122 WOS:000073612000015 WOS000073612000015.pdf 4148837403618207 |
url |
http://dx.doi.org/10.1051/analusis:1998122 http://hdl.handle.net/11449/31290 |
identifier_str_mv |
Analusis. Les Ulis Cedexa: E D P Sciences, v. 26, n. 3, p. 130-134, 1998. 0365-4877 10.1051/analusis:1998122 WOS:000073612000015 WOS000073612000015.pdf 4148837403618207 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Analusis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
130-134 application/pdf |
dc.publisher.none.fl_str_mv |
E D P Sciences |
publisher.none.fl_str_mv |
E D P Sciences |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964722667192320 |